One Pint BRF Cake

AGAMA · 08-19-09, 22:34

I have to use one pint, large mouth canning jars instead of half pints, due to local unavailability and laziness. What differences should I expect to see? Like slower colonizing, etc... Just doubled everything, except spore solution. I used 1 cc per hole(4 cc/ jar).
Thanx.

apokalypse · 08-19-09, 22:36

I always do pint jars, double the bang for your buck (also double the loss if they contam

)

My colonizations usually take 2-3 weeks for a full colonization. But you were right to double the ingredients, that's all there is to it!

I use 1/4 cc per hole, 1cc for each jar. 4cc/jar seems like overkill...

AGAMA · 08-19-09, 22:43

Gotcha apokalypse. Will back off some on the amount of liquid goodies on next try.
Appreciate the info.

(PS thanx for moving this thread)

apokalypse · 08-19-09, 22:53

^_^ wasn't me, I'm not a mod

It does seem a little silly to only use 1cc when you double the amount of substrate, but the spores know how to do what they do, so you don't need to splurge

I usualy get a 10cc syringe to last over a dozen jars, you just gotta take it slowly the first few jars til you have the same # of cc's and jars left.

GadgetGuy · 08-20-09, 02:56

I agree with Apokalypse on the injection. I as well have been using mostly full pint jars and I'm getting em colonized in 7 days from liquid culture

Love em!

coyoteyogi · 08-20-09, 06:07

As my avatar shows I use pint jars too. More nutes = more shrooms = more time. On this latest batch, a FOAF is using both. Full report when he has something to show for it.

AGAMA · 08-20-09, 07:58

Quote:

Originally Posted by GadgetGuy

I agree with Apokalypse on the injection. I as well have been using mostly full pint jars and I'm getting em colonized in 7 days from liquid culture

Love em!

WOW. A week? Now I've got a goal! My colo rates are much slower. I probably need to up the heat and see. Projects are staying on the cool end at ~70 for fruiting and ~75 for colonizing, without any assistance from me.

AGAMA · 08-20-09, 07:59

Quote:

Originally Posted by coyoteyogi

As my avatar shows I use pint jars too. More nutes = more shrooms = more time. On this latest batch, a FOAF is using both. Full report when he has something to show for it.

That'd be cool. The more info, the better!

Outer · 08-20-09, 08:59

Quote:

Originally Posted by AGAMA

WOW. A week? Now I've got a goal! My colo rates are much slower. I probably need to up the heat and see. Projects are staying on the cool end at ~70 for fruiting and ~75 for colonizing, without any assistance from me.

A week for pint BRF cakes? or popcorn? A week for brf in a pint is pretty awsome fast.

TastyBeverage · 08-20-09, 10:09

Quote:

Originally Posted by Outer

A week for brf in a pint is pretty awsome fast.

That's the beauty of an LC !

Read all about 'em right here:

http://forums.mycotopia.net/liquid-c...ey-dextrose-q/

Ben Dover · 08-20-09, 10:11

Quote:

Originally Posted by AGAMA

WOW. A week? Now I've got a goal! My colo rates are much slower. I probably need to up the heat and see. Projects are staying on the cool end at ~70 for fruiting and ~75 for colonizing, without any assistance from me.

GG could be using Hip's supercake formula. If he is not and it is a fast isolate, a supercake could knock a couple days off that time.

Those temps are not to bad considering thats what they are without your interference. A heatbomb seems like it would work well in your situation.

MLBjammer · 08-20-09, 10:29

Yeah, those temps are okay. I don't use any incubator, and most of my BRF jars finish in less than three weeks from MS, and my popcorn jars take maybe two weeks. As long as you have healthy, steady growth, you are in good shape.

Sideshow · 08-20-09, 19:10

I can't consistently colonize a 1/2 pint in a week, much less a full 1/2 pint - and I use 10cc per jar in 6 holes.
Kudos to those who can.

toadshroom · 08-27-09, 15:09

Quote:

Originally Posted by Sideshow

I can't consistently colonize a 1/2 pint in a week, much less a full 1/2 pint - and I use 10cc per jar in 6 holes.
Kudos to those who can.

you use 10cc's per jar? that can't be right...

Sideshow · 08-27-09, 20:03

It is right, for me at least.
Heres a few examples:

These are jars of Hillbilly from LC, nocced on 8/17.
I took one pic with flash, one without.

Heres a closeup of one jar, I flipped it right before the picture was taken.

If 'm feelin lazy, I will just use a standard plastic or metal PF-type lid.
Most of the time, I use 1 layer of postal tyvek over the normal verm barrier. I think it provides better air exchange and is easy to work with. It stays completely sealed untill breached by the needle, which makes me feel better about cleanliness, as well. I doo keep the lids loosely covered with foil until about 80% colonized.

This is a full pint of PFA which I nocced up, idk, 3 weeks ago? I'll birth it, smash and case it in its owns small tray in approximately 3 days, when I will do the same with the 3 hillbilly jars above.

If anyone has any suggestions, I would be glad for the assistance.

MLBjammer · 08-27-09, 20:08

That is a ton of inoculant. The fastest I have had BRF jars colonize personally was in about two weeks--from LC at incubation temps.

Do you make your cakes a little dry to account for the extra inoculant?

Those aren't bad colonization times at all, IMO. Gotta love the Hillbilly.

Sideshow · 08-27-09, 20:43

I forgot to say before that:

I don't incubate, they are prolly around 77-83 degrees in my closet, but the temps have sung wide day/night recently.

I use the std 2:1:1 ratio -verm/brf & corn meal/h20 mix. I know its alot of water, you may be able to see the moisture from pics. These additives used, a tsp each (per doz. jars)- potato flakes, nute yeast, soy isolate.

Hillbilly is quite agressive. I know that PF albino and PE would take at least 1.5x as long to colonize the same amount of sub.

BuckarooBanzai · 08-27-09, 21:35

Icubating at a consistent 84F-85F makes a huge difference in colonization speed.

Starting from a fresh/aggressively growing LC makes a huge difference.

My spore water 1/2 pint cakes usually take 16-20 days to colonize. My LC 1/2 pint cakes usually take 11-14 days to colonize. I haven't done any full pint cakes...

Cakes are beautiful things, very much overlooked in this hobby I think. I frequently use PF cakes (supercake recipe) when I start from spores. It is so easy to isolate a single PF style cake and keep it clean. Great source for clone tissue and clean spores.

AGAMA · 08-28-09, 00:46

Quote:

Originally Posted by BuckarooBanzai

It is so easy to isolate a single PF style cake and keep it clean. Great source for clone tissue and clean spores.

Please explain your method of obtaining clone tissue this way.

What do you mean 'clean' spores?

BTW, thanx BB, SS, MLBjammer, and every one of you who is aiding in my online education.

P.S. How do I get a saying on the bottom of my posts like on almost everyone else's?

whatchamacallit · 08-28-09, 05:03

Quote:

Originally Posted by AGAMA

P.S. How do I get a saying on the bottom of my posts like on almost everyone else's?

User CP, edit signature..

MLBjammer · 08-28-09, 06:17

If you isolate a cake in its own FC, it is much cleaner than if mingling with other cakes, casings, etc., in a larger FC. Thus, tissue from fruits and spore prints would be less tainted by other variables.

As far as cloning, there are many ways. You can grow a sample in an LC, drop tissue in a jar with glass shards and water and make syringes, grow tissue out on cardboard, on and on. The cloning section in the vaults is very thorough.

Peace.

BuckarooBanzai · 08-28-09, 09:14

Are you familiar with Chronic's invitro Tek?
http://archives.mycotopia.net/discus...tml?1012857760

I do a variant with silicone injection ports and double Tyvek vents on the lids. The jar is never opened until after it has fruited (inside the jar). There is enough air exchange to fruit, but all the air is filtered by passing through two layers of Tyvek.

When the jar is finally opened, the mushroom material inside is sterile. If you work with a laminar flow station or a flow hood, cloning is painlessly easy. You just cut a little tissue with sterile scissors and drop it into the LC (or onto agar, or into a popcorn jar).

When you print an invitro fruit in front of a flow hood, you are guaranteed of having a totally clean print. I store prints in the corner of a freezer weight baggie that has been heat sealed. I make syringes by squirting a little clean water in the baggie, scraping the print with a syringe needle and sucking up the purple spore water. Since the print gets washed down, any contams on it would end up in the spore solution.

I have found that every strain (that I have worked with) which does well invitro will do very well on bulk sub. When I get ready to start a new strain run, I do 8-10 invitro jars. The best two of those are used for clone material to start the next generation.

GadgetGuy · 08-28-09, 17:21

Quote:

Originally Posted by AGAMA

WOW. A week? Now I've got a goal! My colo rates are much slower. I probably need to up the heat and see. Projects are staying on the cool end at ~70 for fruiting and ~75 for colonizing, without any assistance from me.

I make my PF mixture just slightly drier than the original tek calls for. Not much but just a tiny bit like just under the 1 cup line and put it in the jars as fluffy as I can possibly get it in there. Then I do about 1/4 cc into 4 innoculation holes and run the needle side to side as I inject the LC in to spread it out a little bit more. Usually within two days I have myc blotches that are almost touching on the sides and almost meeting on the bottom of the jar. 1 pint PF cakes kick ass for spawning to hpoo/verm too. I incubate at 80f for the most part, not sure if that helps speed em up even more.

AGAMA · 08-28-09, 19:53

Quote:

Originally Posted by BuckarooBanzai

When the jar is finally opened, the mushroom material inside is sterile. If you work with a laminar flow station or a flow hood, cloning is painlessly easy. You just cut a little tissue with sterile scissors and drop it into the LC (or onto agar, or into a popcorn jar).

When you print an invitro fruit in front of a flow hood, you are guaranteed of having a totally clean print. I store prints in the corner of a freezer weight baggie that has been heat sealed. I make syringes by squirting a little clean water in the baggie, scraping the print with a syringe needle and sucking up the purple spore water.
I have found that every strain (that I have worked with) which does well invitro will do very well on bulk sub. When I get ready to start a new strain run, I do 8-10 invitro jars. The best two of those are used for clone material to start the next generation.

I stand in amazement! Thanx for giving me a direction to go to learn more. At the moment, I have no GB or flowhood of any kind. Just spray surfaces and air with hospital disinfectant and use a lot of alcohol. It's working so far, but about time to upgrade. Again: Thanx

MLBjammer · 08-28-09, 20:09

You can take a cheap storage container and make a decent GB. Take a small-size coffee can and heat the bottom on the stove, press it through the bottom of the container for two pretty nice arm holes--you will have to heat it twice for like 30 seconds to a minute, once for each hole. Press the can slowly and steadily so that it doesn't crack the plastic. And there you have a GB for about five bucks!

Get yourself some long gloves (wipe heavy with ISO alcohol before putting them in the GB), bleach bomb the GB before use, and you are set.

Buckaroo is--as I have always said--one of the best!

AGAMA · 08-28-09, 20:17

Quote:

Originally Posted by GadgetGuy

1 pint PF cakes kick ass for spawning to hpoo/verm too. I incubate at 80f for the most part, not sure if that helps speed em up even more.

I gotta get my temps up just a hair more.

I guess I'll just have to find out for myself about the hpoo/verm thing, eh? This is wonderful news. The direction to go next is now more clear.

I appreciate the interest you've shown in what I'm doing. Best of luck in all you do!!! Thanx

MLBjammer · 08-29-09, 03:29

Hpoo is great because mycelium loves it, and it resists contams fairly well once colonized, and there are plenty of nutes. I like to mix the poo with coir and just a little vermiculite myself. If you have nice aged stuff, you don't really have to mix anything else.

toadshroom · 08-29-09, 06:41

Quote:

Originally Posted by Sideshow

I forgot to say before that:

I don't incubate, they are prolly around 77-83 degrees in my closet, but the temps have sung wide day/night recently.

I use the std 2:1:1 ratio -verm/brf & corn meal/h20 mix. I know its alot of water, you may be able to see the moisture from pics. These additives used, a tsp each (per doz. jars)- potato flakes, nute yeast, soy isolate.

Hillbilly is quite agressive. I know that PF albino and PE would take at least 1.5x as long to colonize the same amount of sub.

You colonize and fruit within the same temperature range?

Sideshow · 08-29-09, 10:23

Yes, same temp range

BuckarooBanzai · 08-29-09, 11:15

That will work. You can incubate in cooler temps - but it takes a lot longer for projects to finish.

AGAMA · 08-29-09, 16:44

Quote:

Originally Posted by BuckarooBanzai

That will work. You can incubate in cooler temps - but it takes a lot longer for projects to finish.

I'm finding that out to be very true. Will be constructing a heatbomb as soon as I can find a cheap adjustable fish tank heater.

BuckarooBanzai · 08-29-09, 17:49

Do you have a refrigerator or TV? Lots of different appliances generate heat. The computer monitor you are looking is one!

If you wrap a cardboard box in a blanket and put it on top of the fridge, it will be considerably warmer than the surrounding environment.

AGAMA · 08-30-09, 00:51

I'll get busy brainstorming my heat sources. Thanx for the ideas BB.
Also got to get better temp tracking. Am currently using two meat thermometers! Ya go with whatcha got.
Pt jars are almost colonized.
Innoculated some more pts today.
Time to learn more about LC and spore syringes!
Pics coming soon.

AGAMA · 09-01-09, 20:23

A small heater on for 15 minutes every two hours now keeps colo temp at 80 deg and the fruiting bulk sub at a level 76. This I can work with. BRF pts are colonizing at a much improved rate(see below). Will soon have to decide on what mix of hpoo, verm, and/or straw to go with. Got a bale of this year's straw to work with, so think I'll use some of it.

GadgetGuy · 09-01-09, 20:28

Quote:

Originally Posted by Outer

A week for pint BRF cakes? or popcorn? A week for brf in a pint is pretty awsome fast.

Yes a week for a 1 pint BRF cake pretty consistently for me. A little dryer substrate and a good healthy amount of fresh LC. I guess you could really call it 10 days because after they look fully colonized I wait 3 days to use them for spawn but visually yes, 1 week give or take a day depending on how that jar feels.

BuckarooBanzai · 09-01-09, 21:14

A warm incubator makes a huge difference in speed. A cake at 79F will take well over three times as long to finish as a cake at 85F.

LC makes a difference too. Spores have to germinate and that takes anywhere from 2-5 days on cakes. Perhaps up to six days before you can easily see growth. You can almost always see LC growth within 3 days on cakes (2 on grains).

And LCs are just fun to play with...

fungusfarmer · 09-01-09, 21:21

I use the styroffoam coolers Omaha steaks ship their stuff in. Heavy styrofaom, stackable, luv'em

GadgetGuy · 09-01-09, 21:29

Quote:

Originally Posted by BuckarooBanzai

A warm incubator makes a huge difference in speed. A cake at 79F will take well over three times as long to finish as a cake at 85F.

LC makes a difference too. Spores have to germinate and that takes anywhere from 2-5 days on cakes. Perhaps up to six days before you can easily see growth. You can almost always see LC growth within 3 days on cakes (2 on grains).

And LCs are just fun to play with...

Yep. Mine sits at about 80f and when it gets warm through the day it may reach about 84f and LC on BRF cakes or popcorn grow FAST at those temps. Much quicker than I saw at room temp which is about 70-75 80 at best during the day. Temp makes a HUGE difference for me.

masterplain88 · 09-01-09, 22:03

yea the 50/50 hpoo/straw worked real nice for me i spawned it with popcorn at about a 1:1 or 1:2 ratio.

Quote:

Originally Posted by AGAMA

A small heater on for 15 minutes every two hours now keeps colo temp at 80 deg and the fruiting bulk sub at a level 76. This I can work with. BRF pts are colonizing at a much improved rate(see below). Will soon have to decide on what mix of hpoo, verm, and/or straw to go with. Got a bale of this year's straw to work with, so think I'll use some of it.

GadgetGuy · 09-01-09, 22:20

Quote:

Originally Posted by Ben Dover

GG could be using Hip's supercake formula. If he is not and it is a fast isolate, a supercake could knock a couple days off that time.

Those temps are not to bad considering thats what they are without your interference. A heatbomb seems like it would work well in your situation.

No isolate. Just a little dryer than normal old PF tek and a larger amount of LC solution. Maybe I've gotten lucky lately but the PF jars have been kicking ass with my B+ LC. I have a GT LC on the way so we will see if it works as quickly. I still can't see it taking more than 14 days on a slow MS LC to get a jar colonized with a high innoc amount. Im under the impression B+ is somewhat aggressive from what I've seen, but the visual aspect leaves a little to be desired.

AGAMA · 09-01-09, 22:39

Quote:

Originally Posted by masterplain88

yea the 50/50 hpoo/straw worked real nice for me i spawned it with popcorn at about a 1:1 or 1:2 ratio.

Think I'll do the 50/50 hpoo/straw. The 50/50 hpoo/verm is kinkin' butt!

Can one crumble pts of BRF into and mix with straw alone(haven't done my homework on this one, just thought of it)?

GadgetGuy · 09-01-09, 23:33

50/50 is a kick ass ratio. The spawn used is well worth the colonization time compared to inviting the bad guys in.....

AGAMA · 09-01-09, 23:48

Quote:

Originally Posted by GadgetGuy

50/50 is a kick ass ratio. The spawn used is well worth the colonization time compared to inviting the bad guys in.....

Hey GG, have you personally spawned BRF cakes to hpoo/verm or hpoo/straw? Thought I'd glean some wisdom(haven't spent time in the vaults for a few days) from someone who's "been there".

GadgetGuy · 09-02-09, 02:50

Quote:

Originally Posted by AGAMA

Hey GG, have you personally spawned BRF cakes to hpoo/verm or hpoo/straw? Thought I'd glean some wisdom(haven't spent time in the vaults for a few days) from someone who's "been there".

I just recently retrieved my first straw bales ever but as far as spawning PF cakes to Hpoo/verm, I can say yes.

http://forums.mycotopia.net/fungi-gr...-qt-tub-b.html (Just spawned a 30 qt tub with B+)

Works like a charm for me. Seems so far with my limited experience to work better than grains as far as reliability...

masterplain88 · 09-02-09, 03:11

damn thats kinda a pricey mix. how much you pay for your verm.?
my first bulk i used straw that cost me about $3 and the hpoo was free.
im kinda unsure of what bulk to try next im thinking something like hpoo/straw/verm/coir or llike coir/castings/verm or straw or what. but i want to try coir for sure and try to leave hpoo out. kinda hard to pasturize poo with chick in the house.putin poo were your food is. im thinking maybe one of those steal old milk cans with a propain burner under it but i like the oven pasturization tek better.
masterplain88

GadgetGuy · 09-02-09, 03:35

I bought an exceedingly large bag of verm 6 years ago that hasn't gone away yet. Not sure of the size as Im sure its measured in volume but it has to be twice the size of a 50lb bag of dog food. I think it cost me 14 bucks US. Can't remember. It hasn't disappeared very quickly for me and has easily outwieghted its cost in fun for me.

masterplain88 · 09-02-09, 03:58

yea i use to get it for like 5 bucks for those 8qt bags then that place stoped carrying them so now i am paying like 8-9 for the same size bag. they did have the huge bag like what your are talking about one of those big feed sack meterial bags that says vermiculit in block letters on it. i think that is going t be my next buy.
masterplain88

GadgetGuy · 09-02-09, 04:01

I don't know what size it is but as a guess according to my tubs I would guess it as at least 300 quarts. Its a big bag. I think its actually measured in cubic meters.

AGAMA · 09-02-09, 08:45

Quote:

Originally Posted by GadgetGuy

I don't know what size it is but as a guess according to my tubs I would guess it as at least 300 quarts. Its a big bag. I think its actually measured in cubic meters.

Got a 4 cubic foot bag(looks twice the size of a 50# sack) of verm locally at a nursery for $16. It's gonna take a LONG time to go through that.
The first thing he asked was "what do you want vermiculite for anyway. I told him I was mixing up a custom potting soil for my wife from a formula she was given. He seemed satisfied.

GadgetGuy · 09-02-09, 15:55

Quote:

Originally Posted by AGAMA

Got a 4 cubic foot bag(looks twice the size of a 50# sack) of verm locally at a nursery for $16. It's gonna take a LONG time to go through that.
The first thing he asked was "what do you want vermiculite for anyway. I told him I was mixing up a custom potting soil for my wife from a formula she was given. He seemed satisfied.

Yep that would be the bag. Has lasted a LONG time and was cheaper than dirt, literally.

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08-19-09, 22:34	#1 (permalink)
AGAMA cuban jester Join Date: Jun 2009 Posts: 229	One Pint BRF Cake I have to use one pint, large mouth canning jars instead of half pints, due to local unavailability and laziness. What differences should I expect to see? Like slower colonizing, etc... Just doubled everything, except spore solution. I used 1 cc per hole(4 cc/ jar). Thanx.

08-19-09, 22:36	#2 (permalink)
apokalypse Dr. Durgs Join Date: Apr 2005 Posts: 1,456	I always do pint jars, double the bang for your buck (also double the loss if they contam ) My colonizations usually take 2-3 weeks for a full colonization. But you were right to double the ingredients, that's all there is to it! I use 1/4 cc per hole, 1cc for each jar. 4cc/jar seems like overkill... __________________ "Soo, babies don't come out the butt?"

08-19-09, 22:53	#4 (permalink)
apokalypse Dr. Durgs Join Date: Apr 2005 Posts: 1,456	^_^ wasn't me, I'm not a mod It does seem a little silly to only use 1cc when you double the amount of substrate, but the spores know how to do what they do, so you don't need to splurge I usualy get a 10cc syringe to last over a dozen jars, you just gotta take it slowly the first few jars til you have the same # of cc's and jars left. __________________ "Soo, babies don't come out the butt?"

08-20-09, 02:56	#5 (permalink)
GadgetGuy Stupid Snowboarder... Join Date: Feb 2009 Posts: 1,311	I agree with Apokalypse on the injection. I as well have been using mostly full pint jars and I'm getting em colonized in 7 days from liquid culture Love em! __________________ I have to hold on to the grass or I'll fall off the world!

08-19-09, 22:43	#3 (permalink)
AGAMA cuban jester Join Date: Jun 2009 Posts: 229	Gotcha apokalypse. Will back off some on the amount of liquid goodies on next try. Appreciate the info. (PS thanx for moving this thread)

08-20-09, 06:07	#6 (permalink)
coyoteyogi Mycophiliac Join Date: May 2009 Posts: 69	As my avatar shows I use pint jars too. More nutes = more shrooms = more time. On this latest batch, a FOAF is using both. Full report when he has something to show for it.

08-20-09, 10:29	#12 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,864	Yeah, those temps are okay. I don't use any incubator, and most of my BRF jars finish in less than three weeks from MS, and my popcorn jars take maybe two weeks. As long as you have healthy, steady growth, you are in good shape.

08-20-09, 19:10	#13 (permalink)
Sideshow Freakishly Large Join Date: Sep 2007 Posts: 353	I can't consistently colonize a 1/2 pint in a week, much less a full 1/2 pint - and I use 10cc per jar in 6 holes. Kudos to those who can. __________________ Thank you, Mr. Mason

08-27-09, 20:03	#15 (permalink)
Sideshow Freakishly Large Join Date: Sep 2007 Posts: 353	It is right, for me at least. Heres a few examples: These are jars of Hillbilly from LC, nocced on 8/17. I took one pic with flash, one without. Heres a closeup of one jar, I flipped it right before the picture was taken. If 'm feelin lazy, I will just use a standard plastic or metal PF-type lid. Most of the time, I use 1 layer of postal tyvek over the normal verm barrier. I think it provides better air exchange and is easy to work with. It stays completely sealed untill breached by the needle, which makes me feel better about cleanliness, as well. I doo keep the lids loosely covered with foil until about 80% colonized. This is a full pint of PFA which I nocced up, idk, 3 weeks ago? I'll birth it, smash and case it in its owns small tray in approximately 3 days, when I will do the same with the 3 hillbilly jars above. If anyone has any suggestions, I would be glad for the assistance. __________________ Thank you, Mr. Mason

08-27-09, 20:08	#16 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,864	That is a ton of inoculant. The fastest I have had BRF jars colonize personally was in about two weeks--from LC at incubation temps. Do you make your cakes a little dry to account for the extra inoculant? Those aren't bad colonization times at all, IMO. Gotta love the Hillbilly.

08-27-09, 20:43	#17 (permalink)
Sideshow Freakishly Large Join Date: Sep 2007 Posts: 353	I forgot to say before that: I don't incubate, they are prolly around 77-83 degrees in my closet, but the temps have sung wide day/night recently. I use the std 2:1:1 ratio -verm/brf & corn meal/h20 mix. I know its alot of water, you may be able to see the moisture from pics. These additives used, a tsp each (per doz. jars)- potato flakes, nute yeast, soy isolate. Hillbilly is quite agressive. I know that PF albino and PE would take at least 1.5x as long to colonize the same amount of sub. __________________ Thank you, Mr. Mason

08-27-09, 21:35	#18 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	Icubating at a consistent 84F-85F makes a huge difference in colonization speed. Starting from a fresh/aggressively growing LC makes a huge difference. My spore water 1/2 pint cakes usually take 16-20 days to colonize. My LC 1/2 pint cakes usually take 11-14 days to colonize. I haven't done any full pint cakes... Cakes are beautiful things, very much overlooked in this hobby I think. I frequently use PF cakes (supercake recipe) when I start from spores. It is so easy to isolate a single PF style cake and keep it clean. Great source for clone tissue and clean spores. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

08-28-09, 06:17	#21 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,864	If you isolate a cake in its own FC, it is much cleaner than if mingling with other cakes, casings, etc., in a larger FC. Thus, tissue from fruits and spore prints would be less tainted by other variables. As far as cloning, there are many ways. You can grow a sample in an LC, drop tissue in a jar with glass shards and water and make syringes, grow tissue out on cardboard, on and on. The cloning section in the vaults is very thorough. Peace.

08-28-09, 09:14	#22 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	Are you familiar with Chronic's invitro Tek? http://archives.mycotopia.net/discus...tml?1012857760 I do a variant with silicone injection ports and double Tyvek vents on the lids. The jar is never opened until after it has fruited (inside the jar). There is enough air exchange to fruit, but all the air is filtered by passing through two layers of Tyvek. When the jar is finally opened, the mushroom material inside is sterile. If you work with a laminar flow station or a flow hood, cloning is painlessly easy. You just cut a little tissue with sterile scissors and drop it into the LC (or onto agar, or into a popcorn jar). When you print an invitro fruit in front of a flow hood, you are guaranteed of having a totally clean print. I store prints in the corner of a freezer weight baggie that has been heat sealed. I make syringes by squirting a little clean water in the baggie, scraping the print with a syringe needle and sucking up the purple spore water. Since the print gets washed down, any contams on it would end up in the spore solution. I have found that every strain (that I have worked with) which does well invitro will do very well on bulk sub. When I get ready to start a new strain run, I do 8-10 invitro jars. The best two of those are used for clone material to start the next generation. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

08-28-09, 20:09	#25 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,864	You can take a cheap storage container and make a decent GB. Take a small-size coffee can and heat the bottom on the stove, press it through the bottom of the container for two pretty nice arm holes--you will have to heat it twice for like 30 seconds to a minute, once for each hole. Press the can slowly and steadily so that it doesn't crack the plastic. And there you have a GB for about five bucks! Get yourself some long gloves (wipe heavy with ISO alcohol before putting them in the GB), bleach bomb the GB before use, and you are set. Buckaroo is--as I have always said--one of the best!

08-29-09, 03:29	#27 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,864	Hpoo is great because mycelium loves it, and it resists contams fairly well once colonized, and there are plenty of nutes. I like to mix the poo with coir and just a little vermiculite myself. If you have nice aged stuff, you don't really have to mix anything else.

08-29-09, 10:23	#29 (permalink)
Sideshow Freakishly Large Join Date: Sep 2007 Posts: 353	Yes, same temp range __________________ Thank you, Mr. Mason

08-29-09, 11:15	#30 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	That will work. You can incubate in cooler temps - but it takes a lot longer for projects to finish. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

08-29-09, 17:49	#32 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	Do you have a refrigerator or TV? Lots of different appliances generate heat. The computer monitor you are looking is one! If you wrap a cardboard box in a blanket and put it on top of the fridge, it will be considerably warmer than the surrounding environment. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

08-30-09, 00:51	#33 (permalink)
AGAMA cuban jester Join Date: Jun 2009 Posts: 229	Cool I'll get busy brainstorming my heat sources. Thanx for the ideas BB. Also got to get better temp tracking. Am currently using two meat thermometers! Ya go with whatcha got. Pt jars are almost colonized. Innoculated some more pts today. Time to learn more about LC and spore syringes! Pics coming soon. __________________ it is what it is

09-01-09, 20:23	#34 (permalink)
AGAMA cuban jester Join Date: Jun 2009 Posts: 229	A small heater on for 15 minutes every two hours now keeps colo temp at 80 deg and the fruiting bulk sub at a level 76. This I can work with. BRF pts are colonizing at a much improved rate(see below). Will soon have to decide on what mix of hpoo, verm, and/or straw to go with. Got a bale of this year's straw to work with, so think I'll use some of it. Attached Thumbnails __________________ it is what it is

09-01-09, 21:14	#36 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	A warm incubator makes a huge difference in speed. A cake at 79F will take well over three times as long to finish as a cake at 85F. LC makes a difference too. Spores have to germinate and that takes anywhere from 2-5 days on cakes. Perhaps up to six days before you can easily see growth. You can almost always see LC growth within 3 days on cakes (2 on grains). And LCs are just fun to play with... __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-01-09, 21:21	#37 (permalink)
fungusfarmer Mycophage Join Date: Jan 2008 Posts: 103	I use the styroffoam coolers Omaha steaks ship their stuff in. Heavy styrofaom, stackable, luv'em

09-01-09, 23:33	#42 (permalink)
GadgetGuy Stupid Snowboarder... Join Date: Feb 2009 Posts: 1,311	50/50 is a kick ass ratio. The spawn used is well worth the colonization time compared to inviting the bad guys in..... __________________ I have to hold on to the grass or I'll fall off the world!

09-02-09, 03:11	#45 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	damn thats kinda a pricey mix. how much you pay for your verm.? my first bulk i used straw that cost me about $3 and the hpoo was free. im kinda unsure of what bulk to try next im thinking something like hpoo/straw/verm/coir or llike coir/castings/verm or straw or what. but i want to try coir for sure and try to leave hpoo out. kinda hard to pasturize poo with chick in the house.putin poo were your food is. im thinking maybe one of those steal old milk cans with a propain burner under it but i like the oven pasturization tek better. masterplain88

09-02-09, 03:35	#46 (permalink)
GadgetGuy Stupid Snowboarder... Join Date: Feb 2009 Posts: 1,311	I bought an exceedingly large bag of verm 6 years ago that hasn't gone away yet. Not sure of the size as Im sure its measured in volume but it has to be twice the size of a 50lb bag of dog food. I think it cost me 14 bucks US. Can't remember. It hasn't disappeared very quickly for me and has easily outwieghted its cost in fun for me. __________________ I have to hold on to the grass or I'll fall off the world!

09-02-09, 03:58	#47 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	yea i use to get it for like 5 bucks for those 8qt bags then that place stoped carrying them so now i am paying like 8-9 for the same size bag. they did have the huge bag like what your are talking about one of those big feed sack meterial bags that says vermiculit in block letters on it. i think that is going t be my next buy. masterplain88

09-02-09, 04:01	#48 (permalink)
GadgetGuy Stupid Snowboarder... Join Date: Feb 2009 Posts: 1,311	I don't know what size it is but as a guess according to my tubs I would guess it as at least 300 quarts. Its a big bag. I think its actually measured in cubic meters. __________________ I have to hold on to the grass or I'll fall off the world!

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