invitro questions??

mjfrost22 · 08-22-09, 21:47

hello guys i got a question about the invitro i have 2 fully colonized jars of pe now do i just set them on the counter with the light on and loosen the cap or should i remove the alum cover and replace it with tyvek and when do i give them fae? also do they need more h2o?

Dank Side Of The Shroom · 08-22-09, 23:49

yeah just let them get light. as for fae the 4 holes is enough so long as u didnt tape them. dont need loosen covers or anything. gl.

MLBjammer · 08-23-09, 04:40

You can remove the dry vermiculite layer, so there is more head space for growth. Once you see pins, you can fan briefly to speed up growth. But you don't have to.

Gotta love invitro.

mjfrost22 · 08-24-09, 00:33

so once i remove the cap i can fan the top or do i place the jar upside down??

MLBjammer · 08-24-09, 05:05

If you are going to flip it, you want a spacer to keep the cake from blocking the air holes. A small piece of 2" PVC pipe works quite well.

mjfrost22 · 08-27-09, 00:39

just read a thread and saw a pic of an invitro jar with a ziplock bag on it is that good for taking the cap off and growing from the top

apokalypse · 08-27-09, 02:08

So, someone took the lid off of a jar and while it was right-side up they put a ziploc bag over it? Or while it was upside-down? Or are the mushrooms growing invitro supposed to fruit into the bag?

mjfrost22 · 08-27-09, 03:56

ill find the thread actually looks like there friuting from the top of the jar rightside up had a zip lock bag on the top i would assume to get the humidity going and then to take off for fae

MLBjammer · 08-27-09, 04:08

You might as well put them in an FC if you are going to do that, unless you are pressed for space. I would at least poke some small holes in the baggie to allow passive air flow.

mjfrost22 · 08-27-09, 04:13

yeah thats what i thought to i dont know i had a problem with wet bubble on my cakes from the last time i put them in a fc so im looking for another way to fruit im using a pe strain i got from earths tongue this is my second attempt and im failing some how this time they colonized but then had the wet bubble problem and i have 2 jars that where left i decided not to friut the other six got the wet bubble and four more that just finished colonizing so im dont know what to do im under the assumption i have a moldy apt from flooding no carpet laminate floors,(garden apt) just lookin to not have a reaccurring prob

MLBjammer · 08-27-09, 04:24

Oh, yeah, I remember the wet bubble.

I don't know anything about that spore vendor--you should check out TGS or Ralphster's IMO.

Dude, turn a closet into a clean space with some plastic sheeting and a small air purifier. I have grown successfully in some dumps.

If you do use the jars with a ziplock, just fan them a couple times a day.

mjfrost22 · 08-27-09, 04:34

i ordered from sporeworks the 4 pack on sunday as well 10 myco wbs from hippies site so im thinking i can go with a bulk grow and use sandbags once the wbs is colonized so ill just do what i can with what i got now and then start fresh with the new tek and supplies ill try and get my closet workin maybe that will work out

MLBjammer · 08-27-09, 04:45

I had forgotten Sporeworks--excellent as well.

And you are in good hands with Hippie3's bags!

I believe yours will be a much happier story once you have started working with what you have bought.

The sandbags are very contam-resistant for fruiting, BTW. And lots of boomers.

mjfrost22 · 08-27-09, 04:53

yeah ive done the reearch with the old vaults and stuff i also whent with easier strains b+ and GT so im very optamistic about my new project does PE have alot of issue and could the strain have been bad?

MLBjammer · 08-27-09, 05:20

PE is one of the more difficult strains for a beginner to grow. It does not necessarily fruit well from multispore, often producing only a few mature mushrooms per flush. It is also hard to get spore prints from, as the caps don't open as fully as most cubensis strains.

It is possible that you got a tainted syringe; however, it is much more likely that the wet bubble came from something you did. I always blame myself before blaming a syringe made by someone who makes them for a living. One can always be a bit cleaner and more fluid in technique. It just takes practice--quite simply.

GT and B+ are excellent strains. You have chosen well. And you have a treasure trove of information here. Use it well.

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08-22-09, 21:47	#1 (permalink)
mjfrost22 Mycophiliac Join Date: Aug 2009 Posts: 57	invitro questions?? hello guys i got a question about the invitro i have 2 fully colonized jars of pe now do i just set them on the counter with the light on and loosen the cap or should i remove the alum cover and replace it with tyvek and when do i give them fae? also do they need more h2o?

08-22-09, 23:49	#2 (permalink)
Dank Side Of The Shroom Mycotopiate Join Date: Oct 2005 Posts: 3,520	yeah just let them get light. as for fae the 4 holes is enough so long as u didnt tape them. dont need loosen covers or anything. gl. __________________ [SIGPIC][/SIGPIC]

08-27-09, 02:08	#7 (permalink)
apokalypse Dr. Durgs Join Date: Apr 2005 Posts: 1,456	So, someone took the lid off of a jar and while it was right-side up they put a ziploc bag over it? Or while it was upside-down? Or are the mushrooms growing invitro supposed to fruit into the bag? __________________ "Soo, babies don't come out the butt?"

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08-23-09, 04:40	#3 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,877	You can remove the dry vermiculite layer, so there is more head space for growth. Once you see pins, you can fan briefly to speed up growth. But you don't have to. Gotta love invitro.

08-24-09, 00:33	#4 (permalink)
mjfrost22 Mycophiliac Join Date: Aug 2009 Posts: 57	so once i remove the cap i can fan the top or do i place the jar upside down??

08-24-09, 05:05	#5 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,877	If you are going to flip it, you want a spacer to keep the cake from blocking the air holes. A small piece of 2" PVC pipe works quite well.

08-27-09, 00:39	#6 (permalink)
mjfrost22 Mycophiliac Join Date: Aug 2009 Posts: 57	just read a thread and saw a pic of an invitro jar with a ziplock bag on it is that good for taking the cap off and growing from the top

08-27-09, 03:56	#8 (permalink)
mjfrost22 Mycophiliac Join Date: Aug 2009 Posts: 57	ill find the thread actually looks like there friuting from the top of the jar rightside up had a zip lock bag on the top i would assume to get the humidity going and then to take off for fae

08-27-09, 04:08	#9 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,877	You might as well put them in an FC if you are going to do that, unless you are pressed for space. I would at least poke some small holes in the baggie to allow passive air flow.

08-27-09, 04:13	#10 (permalink)
mjfrost22 Mycophiliac Join Date: Aug 2009 Posts: 57	yeah thats what i thought to i dont know i had a problem with wet bubble on my cakes from the last time i put them in a fc so im looking for another way to fruit im using a pe strain i got from earths tongue this is my second attempt and im failing some how this time they colonized but then had the wet bubble problem and i have 2 jars that where left i decided not to friut the other six got the wet bubble and four more that just finished colonizing so im dont know what to do im under the assumption i have a moldy apt from flooding no carpet laminate floors,(garden apt) just lookin to not have a reaccurring prob

08-27-09, 04:24	#11 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,877	Oh, yeah, I remember the wet bubble. I don't know anything about that spore vendor--you should check out TGS or Ralphster's IMO. Dude, turn a closet into a clean space with some plastic sheeting and a small air purifier. I have grown successfully in some dumps. If you do use the jars with a ziplock, just fan them a couple times a day.

08-27-09, 04:34	#12 (permalink)
mjfrost22 Mycophiliac Join Date: Aug 2009 Posts: 57	i ordered from sporeworks the 4 pack on sunday as well 10 myco wbs from hippies site so im thinking i can go with a bulk grow and use sandbags once the wbs is colonized so ill just do what i can with what i got now and then start fresh with the new tek and supplies ill try and get my closet workin maybe that will work out

08-27-09, 04:45	#13 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,877	I had forgotten Sporeworks--excellent as well. And you are in good hands with Hippie3's bags! I believe yours will be a much happier story once you have started working with what you have bought. The sandbags are very contam-resistant for fruiting, BTW. And lots of boomers.

08-27-09, 04:53	#14 (permalink)
mjfrost22 Mycophiliac Join Date: Aug 2009 Posts: 57	yeah ive done the reearch with the old vaults and stuff i also whent with easier strains b+ and GT so im very optamistic about my new project does PE have alot of issue and could the strain have been bad?

08-27-09, 05:20	#15 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,877	PE is one of the more difficult strains for a beginner to grow. It does not necessarily fruit well from multispore, often producing only a few mature mushrooms per flush. It is also hard to get spore prints from, as the caps don't open as fully as most cubensis strains. It is possible that you got a tainted syringe; however, it is much more likely that the wet bubble came from something you did. I always blame myself before blaming a syringe made by someone who makes them for a living. One can always be a bit cleaner and more fluid in technique. It just takes practice--quite simply. GT and B+ are excellent strains. You have chosen well. And you have a treasure trove of information here. Use it well.