Double your spore syringe

[p2k1]

Just a quick tip that might be helpful to some if you've got a spore syringe and and are injecting jars, bags, etc... I always recomend you atleast make a liquid culture for later use and incase something happens to what you inoculated etc...
Anyways, if you save a little bit of of spore water in your syringe (always be sure to shake up before sterilizing and injecting to distribute spores), you can then heat up a bit of honey water and suck it up into the syringe once its cooled and shake it up and in a few days you'll have a syringe full of liquid culture that you can use to inject other jars, liquid cultures etc... this will work faster than injecting with spores.
To make the liquid for your syringe you can put a jar on a scale and zero it, then add 4 grams of honey. You could add 100 grams of water but it might not be great for your scale or you could just measure 100mls of water. To heat it up Im pretty sure you can just microwave although many say you need to pressure cook or steam sterilize. One thing I am curious about is if you can "overcook" the honey rendering it useleess for the mycellium.
Im sure many of you have heard of this before but it might be helpful to someone

[Irishlion]

Thanks

[Soliver]

Originally Posted by p2k1

One thing I am curious about is if you can "overcook" the honey rendering it useleess for the mycellium.
Im sure many of you have heard of this before but it might be helpful to someone

If the liquid is cooked to the extent that the sugars crystalize, then it will
be useless to the mycelium. A safer alternative is potato water, but it's
also cloudy, therefore difficult to tell if contams are present.

My best luck has come from real vanilla extract (I think it was Irish's tek?)
Compared to pure karo, it kicks buttocks.



soliver

[coyoteyogi]

Soliver,
What % vanilla to water? 1:20?

[shortbed454]

i second the question about the vanilla. how do you make this?

[Shmoo]

Thirded the vanilla q, I would definitely like to know.

[hyphaenation]

One that's really easy and is super fast is one with real vanilla extract. Use about a tablespoon per 150mills of water. It's a little dark in color, but is crystal clear and is easy to spot bacteria in. You can PC the cultures for as long as you like as well. 20 minutes @ 15psi is the minimum. No carmelization threat to these cultures.

http://forums.mycotopia.net/fungi-gr...tml#post655667 (Easiest / Best LC Method?)

http://forums.mycotopia.net/liquid-c...es-merged.html (Vanilla Extract and Vented lid tek for liquid cultures! [merged])

Just thought i'd chime in with those.

[GadgetGuy]

Originally Posted by Irishlion

Thanks

Good to see ya man.

[Mr. Kush]

can someone explaine what is meant by real vanilla extract?
are the little bottles sold in grocery stores not the real?
if not where do you purchase?

[trips6928]

Originally Posted by Mr. Kush

can someone explaine what is meant by real vanilla extract?
are the little bottles sold in grocery stores not the real?
if not where do you purchase?

The vanilla is usually sold as vanilla flavoring...Pure vanilla is real, i get Watkins Madagascar Bourbon Pure vanilla extract (35% alcohol) at the grocery store, its a little more expensive but its worth it IMO.

[bumber]

I dont see how it differs that much between karo and vanilla extract...

Vanilla extract-
VANILLA BEAN EXTRACTIVES IN WATER, ALCOHOL (35%), AND CORN SYRUP

Karo light corn syrup-
Ingredients: Light Corn Syrup, Salt, Vanilla

you see? Not saying that you are lying if you say you get better results with vanilla extract, im just saying I dont know why. The alcohol would evaporate during cooking so it has nothing to do with that in the extract, maybe the salt in Karo slows it down? But there is really no salt after you mix with water. I still think your best bet would be pure dextrose mixed with yeast extract and water...

You could, in most cases, take 1cc spore solution where you can see spore clumps and suck up 9cc sterile water and have another full syringe. It only takes 1/4cc to make an LC but the more you use the faster it goes, if your LC is sterile(should be) you can let it sit for a few weeks without contaminating. If you use 1cc in an LC you will only have to leave it 1week, and not let it go more than 10days at incubation temps if growing well.

I think Soliver means not to cook untill the sugars caramalize not crystalize, one means burned sugar the other only means too much sugar in the solution(which shouldn't hurt)

[MLBjammer]

Mycelium seems to really like vanilla. It's kind of like coir, which myc. also has a "taste" for. No one really knows exactly why, but it works quite well. If you don't believe us, just try it.