First ever spore print... kinda light

[bozefonce]

FOAF tried his first ever spore print, used glovebox and Hippie's oven technique to sterilize foil squares. Point is, he thinks he picked the cap a bit too early, and after more than 12 hours the print is only visible through the baggie if you hold it at an angle. Is this still something that can be used to make a syringe? There are definitely spores there, it just doesn't seem like it would be enough to do multiple syringes.

[Sideshow]

Exactly. It will be enough for 1 syringe at least, depending on how light.
Try to get another print with the same cap.

[bozefonce]

Originally Posted by Sideshow

Try to get another print with the same cap.

Yeah, the cap's on the second square now, gonna let it sit longer this time. Probably still won't get a very dark print, but it should be good for one syringe. Thanks for confirming what I had hoped!

[mydarling]

i like to let my prints go 48 hours. they get SUPER dark.

[bozefonce]

Originally Posted by mydarling

i like to let my prints go 48 hours. they get SUPER dark.

Wow, and that looks like it was a huge cap! Very nice The Z strain being used here is producing caps about the size of a quarter, thereabouts.

[masterplain88]

yea once the veil splits it two places i break away the rest of it and they sit on foil for 8-12 hours some time 24 hours to get those real dark nice ones. but normaly 8-12 hours gives a good print.
peace,
masterplain88

[MLBjammer]

I throw out the first print in case it carried any contams with it, and the second one is the keeper for me. Sometimes I can get two.

It doesn't matter if it's dark or not--if you can see spores there are plenty there for your use.

[masterplain88]

agreed. as long as they are visable there is enought and the second one normaly is cleaner.

[P4ulada]

hey mydarling, it´s pretty dark hehe
nice print, I love them.
I do make about 8 spores syringes with it ^^

[BuckarooBanzai]

I generally wait until I see the first dusting of spores on the stem before cutting the cap for printing.

The cap should be completely opened and flattened out across the top.

[Hippie3]

there is no hippie oven tek-
to what are you referring ?

[MLBjammer]

I believe he is referring to your practice of heating foil pieces in an oven to sterilize them. I have read that tip a few times while researching.

[masterplain88]

instead of starting a new thread i am just curious what everybodys procedures are for there cleaning of all the equipment used for making spore prints. (ie) foil,razor blade,glovebox, or jars,plates, and your storing process folded foil, open foil, and bags there stored in fresh 2-1/2 inch by 4 inch bagies ect. and rubbing everything down with iso.
masterplain88

[Shamsu]

Originally Posted by mydarling

i like to let my prints go 48 hours. they get SUPER dark.

Nice Print. Do you consider it better to print onto tinfoil or paper if it isnt going to be used right away?

[BuckarooBanzai]

I sterilize everything that will fit into the pressure cooker and not melt.

My cloning kit is a stainless steel scissors and tweezers as well as a stainless scalpel handle and a stainless probe. I wrap them up in aluminum foil and run them through the PC. Same thing with syringes. When the aluminum foil packets come out, I toss them into a gallon Ziploc to keep dust off the exterior.

The packets are only opened in front of the flow hood and the aluminum foil "cover" is opened up to make a sterile work surface (if needed).

[sandman]

Originally Posted by BuckarooBanzai

I sterilize everything that will fit into the pressure cooker and not melt.

My cloning kit is a stainless steel scissors and tweezers as well as a stainless scalpel handle and a stainless probe. I wrap them up in aluminum foil and run them through the PC. Same thing with syringes. When the aluminum foil packets come out, I toss them into a gallon Ziploc to keep dust off the exterior.

The packets are only opened in front of the flow hood and the aluminum foil "cover" is opened up to make a sterile work surface (if needed).

Nice thats the way to go. Any more info on this "probe" you have? I love gadgets!

[bozefonce]

Originally Posted by Hippie3

there is no hippie oven tek-
to what are you referring ?

Yeah, I know there's a spurious oven tek for flowhood type applications that you have roundly criticized, not referring to that one.

I saw in another thread where you describe wrapping foil squares in another layer of foil, bake at... 400? something like that, for an hour, then place the packet in the glovebox, to be opened inside, where the now sterile squares can be safely used.

[BuckarooBanzai]

It sounds cooler than it is! Just a stainless steel dissecting probe with a triangular head. Good for digging out stem tissue and easy to sterilize with a mini-blowtorch lighter.

Lots of dissecting tools and dentist tools are good for cloning work.