how long can I use my airport syringe

dascylus · 09-02-09, 02:03

I have been reading a lot about liquid cultures and building airport jar lids. The one question I have is, after you pc the airport syringe with the polyfill inside can it be used over and over? I understand that a flame sterilization of the needle is a must, but how long is that good for? Can I seal the end of it with a small circle of tyvek? Would that keep it viable longer?

Thanks in advance.

GadgetGuy · 09-02-09, 02:08

I have used my airport up to 4 times in a week after sterilizaion but after that I get paranoid an re-pc it.

masterplain88 · 09-02-09, 02:47

i kinda eliminated airports. i use silicone ports and pollyfill in my lc's.
gadgetguy what do you use your airports for?
i just use silicone and pollyfill and use polly fill for grain jars then spawn them. i find lc's with the pollyfill in the lid grow in thicker than lc's with just silicone ports.
masterplain88

apokalypse · 09-02-09, 03:00

I used an airport syringe without PCing it with success. Just put in fresh polyfill from the bag and you're good to go. You could also probably soak it in alcohol and let it dry out in the syringe if you wish.

meatwad · 09-02-09, 13:01

Quote:

Originally Posted by masterplain88

i kinda eliminated airports. i use silicone ports and pollyfill in my lc's.
gadgetguy what do you use your airports for?
i just use silicone and pollyfill and use polly fill for grain jars then spawn them. i find lc's with the pollyfill in the lid grow in thicker than lc's with just silicone ports.
masterplain88

how do you shake your LC's without getting your poly wet?

And to the OP, I didn't even know you had to re-starilized the airport lol.. I sterilized mine once and have used it for about a month so far lol

ez_goin · 09-02-09, 14:49

Yeah, I'm not a big fan of sterilization of airport syringes either.

Some people are more willing to risk contamination more than others. It makes perfect sense to be as sterile as possible, and once you have a bad outbreak that stretches out to multiple projects you may not see it necessary to be as clean as some suggest.

With that being said, I don't practice overly sterile procedures still. I am careful to flame needles before ever putting them into a "mother culture", but that is about it. I have pc'd airport syringes just because I had the pc going for other things, but unless that is the case, they don't get pc'd. If I haven't used a syringe in a while and there is nothing in it, I will draw in boiling water and let it sit for a little bit and then eject it. If you do this though, make sure you are not putting much pressure on the syringe as it will deform de to the hot water inside. Also, from my experience, if you tough where the numbers and lines for the cc marks are while there is hot water in a syringe, you will end up wiping off the ink. Try to avoid that as well.

Sorry for the long winded response.

BuckarooBanzai · 09-02-09, 15:00

I have a number of airport syringes. I sterilize them after they are used and come out of the laminar flow station.

I fold them up into a square of aluminum foil and run them through the PC when doing other things. After they are sterilized, I toss the aluminum foil packets into a gallon Ziploc until I need one again.

I sterilize everything I can that touches grain/substrate. I even sterilize the spoon I use to mix up a case.

masterplain88 · 09-02-09, 15:10

well so far i have used a 1pt jar and 2 1 qt jars. only one 1qt had all silicone ports wich you can shake easyer. but my lc with one silicone port and a pollyfill for fae seem to grow in thicker and a lil quiker.for shaking those i dont really shake them like you normally would. i set them on a flat surface and spinn the jar in a circle a bunch of time and it swirls it around and breaks it up a lilttle. for the pollyfill/silicone ports that were a stir plate can come in handy but my do fine just by twisting them on the table.
peace,
mastreplain88

Quote:

Originally Posted by meatwad

how do you shake your LC's without getting your poly wet?

And to the OP, I didn't even know you had to re-starilized the airport lol.. I sterilized mine once and have used it for about a month so far lol

masterplain88 · 09-02-09, 15:18

now i havent mastered the lc tek i just got it down pretty good. but one thing i am kinda woundering about is after i used my bpk lc wich i think i used like 3-4 syringes out of it. from tipping the jar so my needle reaches the lc for the last two i have notice the lc contamed and as far as i can tell the source of it is from the metal(the exposed part around the silicone) that got touched my the lc wen i tipped the jar. so if that was even the source wick it does look like it is should i have spread the silicone around the hole more so it covers the metal on the back side. i just kinda beaded it in ther and the bottom of the lid would have a lil bb of silicone throught it but not smeared over the top of it like you do on the top of the lid. sorry for the runn on.
peace,
masterplain88

GadgetGuy · 09-02-09, 15:52

Quote:

Originally Posted by masterplain88

i kinda eliminated airports. i use silicone ports and pollyfill in my lc's.
gadgetguy what do you use your airports for?
i just use silicone and pollyfill and use polly fill for grain jars then spawn them. i find lc's with the pollyfill in the lid grow in thicker than lc's with just silicone ports.
masterplain88

My LC's just have 2 silicone ports so I can shake the living crap out of them and really get the myc broken up. I use my airport to relieve pressure before innoculating and when I'm drawing up myc into a clean syringe. Keeping airports sterile is no big deal because I just throw them in my PC when I do a batch of jars. I've never ran the PC just to sterilize a syringe.

MLBjammer · 09-02-09, 16:50

Quote:

Originally Posted by masterplain88

now i havent mastered the lc tek i just got it down pretty good. but one thing i am kinda woundering about is after i used my bpk lc wich i think i used like 3-4 syringes out of it. from tipping the jar so my needle reaches the lc for the last two i have notice the lc contamed and as far as i can tell the source of it is from the metal(the exposed part around the silicone) that got touched my the lc wen i tipped the jar. so if that was even the source wick it does look like it is should i have spread the silicone around the hole more so it covers the metal on the back side. i just kinda beaded it in ther and the bottom of the lid would have a lil bb of silicone throught it but not smeared over the top of it like you do on the top of the lid. sorry for the runn on.
peace,
masterplain88

It's not the silicone ports that are causing problems. It is the polyfill filter, I would bet. LCs don't need much air at all.

BuckarooBanzai · 09-02-09, 17:57

A 3/4 filled LC jar will finish quite nicely with just the air in the container. I have seen this happen many, many times.

I don't actively vent LC jars at all, I just never fill them past 3/4 full.

I DO use an airport syringe to bleed off the sterilization vacuum in an LC jar.

masterplain88 · 09-02-09, 18:09

yea i was kinda leaning towards that but when i emptyed the jar to clean it i checked it out. around the silicone port there was like a blackish type mold on the metal that was not covered by silicone and lil starting bits of blk mold in the lc. it could maybe be the pollyfill because like i said i dont have it mastered yet. but im kinda leaning towards the exposed metal because the other 2 polly fill lc are fine. but time will tell.
peace,
masterplain88

Brakiska · 09-02-09, 19:30

been using built-in airport syringe

with the RTV, I put a syringe filled with polyfill
there's ought to be a minimal gas exchange

so, everytime I sterilize my LC, the airport gets a PC rise too
just did a 800 ML LC with 6 TBS of Karo and 3 TBS of Dextrose
its SNOWING in there, with 10 CC of TX within 2 days

built-in takes a bit more space depending on your incubation room, though
let you shake it roughly, too

dascylus · 09-02-09, 23:43

Quote:

Originally Posted by MLBjammer

It's not the silicone ports that are causing problems. It is the polyfill filter, I would bet. LCs don't need much air at all.

aye, not worried about anything other than the vacuum in the jar sucking the whole syringe out. I as others have stated here do not use "max" sterile procedure. I just use common sense, but I like the idea of sterilization ahead of time. I never really thought of storing something after I PC it and taking it out when its time. I usually do everything at once and "make a day of it" Thanks everyone for weighing in.

Phx · 09-02-09, 23:55

Quote:

Originally Posted by MLBjammer

It's not the silicone ports that are causing problems. It is the polyfill filter, I would bet. LCs don't need much air at all.

Quote:

Originally Posted by masterplain88

from tipping the jar so my needle reaches the lc for the last two i have notice the lc contamed and as far as i can tell the source of it is from the metal

I agree with MLB here....also you should rtv both sides of your lids to prevent rust. Some good advice so far. And i only fill my lc's up about a half a jar....although I have only made one batch of 4 lc's so far, I would rather be safe then sorry with my projects....good luck.

Hippie3 · 09-03-09, 23:23

Quote:

Originally Posted by dascylus

...The one question I have is, after you pc the airport syringe with the polyfill inside can it be used over and over?...

yes, you can re-use many times,
i recently tossed one that i'd used proly 50 times
because i could see the polyfil getting dirty.

masterplain88 · 09-04-09, 03:57

yea i use the 1qt they work good the only one that contamed was the 1 pt one. and the polly fill never got wet the metal on the inside of the jar has a weird mold around it and there was mold starting to grow in the lc. but yes i agree both side should have a real thick blob of silicone smeared around to cover it good. i also have a lc with all silicone ports and it works good to. it deff helps if you are trying to be 100% contam resistant as you can. but between the all silicone one and the other 2 the growth is better in the pollyfill ones but they both work good for me.
peace,
masterplain88

Quote:

Originally Posted by Phx

I agree with MLB here....also you should rtv both sides of your lids to prevent rust. Some good advice so far. And i only fill my lc's up about a half a jar....although I have only made one batch of 4 lc's so far, I would rather be safe then sorry with my projects....good luck.

Quote:

Originally Posted by Phx

I agree with MLB here....also you should rtv both sides of your lids to prevent rust. Some good advice so far. And i only fill my lc's up about a half a jar....although I have only made one batch of 4 lc's so far, I would rather be safe then sorry with my projects....good luck.

Alchemist · 09-05-09, 13:13

when you do PC your airport syringes do you PC them with the poly all ready in the syringe or do you PC the syringe and stuff with poly in a glovebox, ect.

BuckarooBanzai · 09-05-09, 13:22

I wrap the airport up in an aluminum foil envelope and fold each edge over three times to keep moisture out.

I never remove the polyfill.

MLBjammer · 09-05-09, 17:11

I would not want to try to stuff polyfill in a syringe inside a GB. I would have a conniption fit.

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09-02-09, 02:03	#1 (permalink)
dascylus Zealot Join Date: Dec 2008 Posts: 22	how long can I use my airport syringe I have been reading a lot about liquid cultures and building airport jar lids. The one question I have is, after you pc the airport syringe with the polyfill inside can it be used over and over? I understand that a flame sterilization of the needle is a must, but how long is that good for? Can I seal the end of it with a small circle of tyvek? Would that keep it viable longer? Thanks in advance. __________________ "I'll climb on your ceiling fan and drop thread in your mouth. Let poison drip down it and silently take you out."

09-02-09, 02:08	#2 (permalink)
GadgetGuy Stupid Snowboarder... Join Date: Feb 2009 Posts: 1,315	I have used my airport up to 4 times in a week after sterilizaion but after that I get paranoid an re-pc it. __________________ I have to hold on to the grass or I'll fall off the world!

09-02-09, 03:00	#4 (permalink)
apokalypse Dr. Durgs Join Date: Apr 2005 Posts: 1,456	I used an airport syringe without PCing it with success. Just put in fresh polyfill from the bag and you're good to go. You could also probably soak it in alcohol and let it dry out in the syringe if you wish. __________________ "Soo, babies don't come out the butt?"

09-02-09, 15:00	#7 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	I have a number of airport syringes. I sterilize them after they are used and come out of the laminar flow station. I fold them up into a square of aluminum foil and run them through the PC when doing other things. After they are sterilized, I toss the aluminum foil packets into a gallon Ziploc until I need one again. I sterilize everything I can that touches grain/substrate. I even sterilize the spoon I use to mix up a case. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-02-09, 02:47	#3 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	i kinda eliminated airports. i use silicone ports and pollyfill in my lc's. gadgetguy what do you use your airports for? i just use silicone and pollyfill and use polly fill for grain jars then spawn them. i find lc's with the pollyfill in the lid grow in thicker than lc's with just silicone ports. masterplain88

09-02-09, 14:49	#6 (permalink)
ez_goin Eager Student Join Date: May 2008 Posts: 435	Yeah, I'm not a big fan of sterilization of airport syringes either. Some people are more willing to risk contamination more than others. It makes perfect sense to be as sterile as possible, and once you have a bad outbreak that stretches out to multiple projects you may not see it necessary to be as clean as some suggest. With that being said, I don't practice overly sterile procedures still. I am careful to flame needles before ever putting them into a "mother culture", but that is about it. I have pc'd airport syringes just because I had the pc going for other things, but unless that is the case, they don't get pc'd. If I haven't used a syringe in a while and there is nothing in it, I will draw in boiling water and let it sit for a little bit and then eject it. If you do this though, make sure you are not putting much pressure on the syringe as it will deform de to the hot water inside. Also, from my experience, if you tough where the numbers and lines for the cc marks are while there is hot water in a syringe, you will end up wiping off the ink. Try to avoid that as well. Sorry for the long winded response.

09-02-09, 15:18	#9 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	now i havent mastered the lc tek i just got it down pretty good. but one thing i am kinda woundering about is after i used my bpk lc wich i think i used like 3-4 syringes out of it. from tipping the jar so my needle reaches the lc for the last two i have notice the lc contamed and as far as i can tell the source of it is from the metal(the exposed part around the silicone) that got touched my the lc wen i tipped the jar. so if that was even the source wick it does look like it is should i have spread the silicone around the hole more so it covers the metal on the back side. i just kinda beaded it in ther and the bottom of the lid would have a lil bb of silicone throught it but not smeared over the top of it like you do on the top of the lid. sorry for the runn on. peace, masterplain88

09-02-09, 17:57	#12 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	A 3/4 filled LC jar will finish quite nicely with just the air in the container. I have seen this happen many, many times. I don't actively vent LC jars at all, I just never fill them past 3/4 full. I DO use an airport syringe to bleed off the sterilization vacuum in an LC jar. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-02-09, 18:09	#13 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	yea i was kinda leaning towards that but when i emptyed the jar to clean it i checked it out. around the silicone port there was like a blackish type mold on the metal that was not covered by silicone and lil starting bits of blk mold in the lc. it could maybe be the pollyfill because like i said i dont have it mastered yet. but im kinda leaning towards the exposed metal because the other 2 polly fill lc are fine. but time will tell. peace, masterplain88

09-02-09, 19:30	#14 (permalink)
Brakiska Mycotopiate Join Date: Mar 2008 Posts: 244	been using built-in airport syringe with the RTV, I put a syringe filled with polyfill there's ought to be a minimal gas exchange so, everytime I sterilize my LC, the airport gets a PC rise too just did a 800 ML LC with 6 TBS of Karo and 3 TBS of Dextrose its SNOWING in there, with 10 CC of TX within 2 days built-in takes a bit more space depending on your incubation room, though let you shake it roughly, too __________________ oOO ¿? OOo

09-05-09, 13:13	#19 (permalink)
Alchemist Mycophiliac Join Date: Jul 2009 Posts: 47	when you do PC your airport syringes do you PC them with the poly all ready in the syringe or do you PC the syringe and stuff with poly in a glovebox, ect.

09-05-09, 13:22	#20 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	I wrap the airport up in an aluminum foil envelope and fold each edge over three times to keep moisture out. I never remove the polyfill. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-05-09, 17:11	#21 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,877	I would not want to try to stuff polyfill in a syringe inside a GB. I would have a conniption fit.

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