cloning mushrooms

p88prw · 09-06-09, 15:42

i have just started the fraction sterilisation process today and the jars will be ready to inoculated in a couple of days time

i have a good idea about what im doing now but am still abit unsure about how to clone the mycelium and mushrooms so that i can isolate them so they can be used in my next grow thanks, ps i am using the mazatapec strain

Dr-Octagon · 09-06-09, 16:16

I dropped mine to agar, let it grow out and made a Karo LC using a recipe from here.

Cut tissue and dropped it in the Karo solution inside a glovebox.

The recipe for the agar i used was agar flakes from a natural food store, potato flakes and some karo.

2 tbl pot flakes, "not reefer" lol
1/4 cu agar flakes
1 tsp corn syrup
1 cu water.

This recipe will make more than you really need. I sorta 1/4'rd it. I dont have petri's, i used small jars with airport in the lids.

For the Lc, i used 1 tsp Karo per 100 mls H2o

BuckarooBanzai · 09-06-09, 16:44

Cloning means taking a small amount of sterile tissue from an adult mushroom and growing it out on agar, in LC or on grain.

masterplain88 · 09-06-09, 19:48

so you can clone and or isolate a strain and put the agar wedg to an lc and your islate can be used that way of does it have to go from the agar to your substrate.?
masterplain88

BuckarooBanzai · 09-06-09, 22:27

Once myc is growing on agar, you can move it to any media you desire - agar, grain or LC.

You can use a syringe and create a slurry of water/myc to inoculate agar, grain or LC.

masterplain88 · 09-06-09, 22:42

what would you say is the best agar to get from spore works.?
mastreplain88

BuckarooBanzai · 09-06-09, 22:54

I like the classic Potato Dextrose Agar (PDA) from sporeworks.

I've used it many, many times.

masterplain88 · 09-07-09, 00:06

does anybody know the diffrence between the jars of agar and the presteralize mason jars are they both the same why the diff price.?
thanks,
masterplain88

BuckarooBanzai · 09-07-09, 09:57

The Mason jar is pre-mixed and pre-sterilized agar. You put in hot water until it melts and then pour your plates. A Mason jar will make about 20-30 plates.

The jars of agar are just powder. You add water, heat and sterilize. Then you pour plates. The jar will make around 7 liters of agar (about 14 Mason jars).

The powder is more expensive because it makes a lot more agar.

pihop · 09-07-09, 14:42

I would recommend cardboard to clone on.

Easy to work with and doesn't contaminate as easily as agar. I've had a bunch of agar plates go green in a couple days. I've only done 3 tries with cardboard but it has never contaminated for me. I had one piece of wet cardboard lying around for almost a month and no contamination.

I have to add that I have never sterilized the cardboard.

This is my most serious try yet, works like a charm.
http://forums.mycotopia.net/fungi-gr...cture-log.html (Cloning Z ---> 1st flush, your help needed [Picture Log])

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09-06-09, 15:42	#1 (permalink)
p88prw Mycophiliac Join Date: Aug 2009 Posts: 43	cloning mushrooms i have just started the fraction sterilisation process today and the jars will be ready to inoculated in a couple of days time i have a good idea about what im doing now but am still abit unsure about how to clone the mycelium and mushrooms so that i can isolate them so they can be used in my next grow thanks, ps i am using the mazatapec strain __________________ i hate to advocate drugs, alcohol, violance or insanity to anyone, but they've always worked for me!

09-06-09, 16:44	#3 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	Cloning means taking a small amount of sterile tissue from an adult mushroom and growing it out on agar, in LC or on grain. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-06-09, 22:27	#5 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	Once myc is growing on agar, you can move it to any media you desire - agar, grain or LC. You can use a syringe and create a slurry of water/myc to inoculate agar, grain or LC. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-06-09, 22:54	#7 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	I like the classic Potato Dextrose Agar (PDA) from sporeworks. I've used it many, many times. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-06-09, 16:16	#2 (permalink)
Dr-Octagon Mycophiliac Join Date: Jul 2008 Posts: 38	I dropped mine to agar, let it grow out and made a Karo LC using a recipe from here. Cut tissue and dropped it in the Karo solution inside a glovebox. The recipe for the agar i used was agar flakes from a natural food store, potato flakes and some karo. 2 tbl pot flakes, "not reefer" lol 1/4 cu agar flakes 1 tsp corn syrup 1 cu water. This recipe will make more than you really need. I sorta 1/4'rd it. I dont have petri's, i used small jars with airport in the lids. For the Lc, i used 1 tsp Karo per 100 mls H2o

09-06-09, 19:48	#4 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	so you can clone and or isolate a strain and put the agar wedg to an lc and your islate can be used that way of does it have to go from the agar to your substrate.? masterplain88

09-06-09, 22:42	#6 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	what would you say is the best agar to get from spore works.? mastreplain88

09-07-09, 00:06	#8 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	does anybody know the diffrence between the jars of agar and the presteralize mason jars are they both the same why the diff price.? thanks, masterplain88

09-07-09, 09:57	#9 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	The Mason jar is pre-mixed and pre-sterilized agar. You put in hot water until it melts and then pour your plates. A Mason jar will make about 20-30 plates. The jars of agar are just powder. You add water, heat and sterilize. Then you pour plates. The jar will make around 7 liters of agar (about 14 Mason jars). The powder is more expensive because it makes a lot more agar. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-07-09, 14:42	#10 (permalink)
pihop Necrophiliac Join Date: Jan 2009 Posts: 294	I would recommend cardboard to clone on. Easy to work with and doesn't contaminate as easily as agar. I've had a bunch of agar plates go green in a couple days. I've only done 3 tries with cardboard but it has never contaminated for me. I had one piece of wet cardboard lying around for almost a month and no contamination. I have to add that I have never sterilized the cardboard. This is my most serious try yet, works like a charm. http://forums.mycotopia.net/fungi-gr...cture-log.html (Cloning Z ---> 1st flush, your help needed [Picture Log]) __________________ Current grow: Orissa India

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