Corn Syrup Agar Recipe

Kreat · 09-09-09, 08:32

I remember seeing someplace a light corn syrup agar recipe but I can't seem to find it right now. Does anyone know what this recipe entails? Or does anyone have another basic agar recipe I can use with household items?

Irishlion · 09-09-09, 08:36

http://forums.mycotopia.net/agar-str...lustrated.html (Homemade PDA+ Agar Plates Illustrated)

Bro we have the vaults for a reason, check them out, might find alot more in there.

Kreat · 09-09-09, 08:39

does the search feature search the vaults too?

I wasn't thinking about PDA stupid me, that works perfectly since I use PD for my LC's.

designlogic · 09-09-09, 14:50

Here's another thread which has a recipe for homemade agar which you may be interested in. Everything needed is available at the local grocery store.
http://forums.mycotopia.net/fungi-gr...isolation.html (help with agar substitute isolation)

BuckarooBanzai · 09-09-09, 16:31

A good rule of thumb with agar and LCs is to shoot for a concentration of 4%-5% sugar (by mass).

Kreat · 09-09-09, 17:20

Quote:

Originally Posted by designlogic

Here's another thread which has a recipe for homemade agar which you may be interested in. Everything needed is available at the local grocery store.
http://forums.mycotopia.net/fungi-gr...isolation.html (help with agar substitute isolation)

Thank you that looks like it might work perfectly!

Quote:

Originally Posted by BuckarooBanzai

A good rule of thumb with agar and LCs is to shoot for a concentration of 4%-5% sugar (by mass).

I guess this is the best piece of advice that I could use. How much agar would you want in your concentration?

masterplain88 · 09-09-09, 17:28

im getting reading to do agar and i am weight mine on scales and useing those measurments in the jar/pc isal in the store gatta look around a lil.
masetrplain88

BuckarooBanzai · 09-09-09, 17:33

If you are using lab grade agar, 15gm per liter of water should be enough to form a solid gel.

If you are using food grade agar, you might want 20gm-25gm per liter of water. Food grade should have a recipe on the package, though - just follow the "hard gel" prep instructions.

masterplain88 · 09-09-09, 18:02

i have read a lot or agar teks i am thinking of making my own. should i try more than one kind for like a experiment i think i am going to do a lot of agar work like 50-69 trays of so ones i do my first 20. sorry for the thread jack but.http://www.sc.mahidol.ac.th/scbt/aca...0Web/media.htm

masterplain88

BuckarooBanzai · 09-09-09, 20:08

Try to stick to no more than 4%-5% sugar for good fungi agar. That is the only real "rule." And that isn't really a rule, more or less will work.

Best agar recipe depends on what you are doing with it.

Long term storage agar (in a slant or a petri) should be jacked up full of nutrition. An Amaranth/oatmeal/bee pollen/dog food provides a nice spectrum of nutes for storage.

If you are just growing out cultures, testing samples or isolating - a basic LME or dextrose agar is your best choice.

Agar for quick temporary growth should not have a lot of "extra" nutrients - those nutes could feed contams.

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09-09-09, 08:32	#1 (permalink)
Kreat Mycotopiate Join Date: Mar 2006 Posts: 249	Corn Syrup Agar Recipe I remember seeing someplace a light corn syrup agar recipe but I can't seem to find it right now. Does anyone know what this recipe entails? Or does anyone have another basic agar recipe I can use with household items? __________________ Favorite LC Tek Critter Country Tek

09-09-09, 08:36	#2 (permalink)
Irishlion KEY MASTER Join Date: May 2008 Posts: 3,343	http://forums.mycotopia.net/agar-str...lustrated.html (Homemade PDA+ Agar Plates Illustrated) Bro we have the vaults for a reason, check them out, might find alot more in there. __________________ a man without honor isn't a man at all but, a soul trapped in a flesh body that is lost in a void of its own making

09-09-09, 08:39	#3 (permalink)
Kreat Mycotopiate Join Date: Mar 2006 Posts: 249	does the search feature search the vaults too? I wasn't thinking about PDA stupid me, that works perfectly since I use PD for my LC's. __________________ Favorite LC Tek Critter Country Tek

09-09-09, 16:31	#5 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	A good rule of thumb with agar and LCs is to shoot for a concentration of 4%-5% sugar (by mass). __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-09-09, 14:50	#4 (permalink)
designlogic Researcher Join Date: May 2009 Posts: 267	Here's another thread which has a recipe for homemade agar which you may be interested in. Everything needed is available at the local grocery store. http://forums.mycotopia.net/fungi-gr...isolation.html (help with agar substitute isolation)

09-09-09, 17:28	#7 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	im getting reading to do agar and i am weight mine on scales and useing those measurments in the jar/pc isal in the store gatta look around a lil. masetrplain88

09-09-09, 17:33	#8 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	If you are using lab grade agar, 15gm per liter of water should be enough to form a solid gel. If you are using food grade agar, you might want 20gm-25gm per liter of water. Food grade should have a recipe on the package, though - just follow the "hard gel" prep instructions. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

09-09-09, 18:02	#9 (permalink)
masterplain88 Mycotopiate Join Date: Jan 2009 Posts: 683	i have read a lot or agar teks i am thinking of making my own. should i try more than one kind for like a experiment i think i am going to do a lot of agar work like 50-69 trays of so ones i do my first 20. sorry for the thread jack but.http://www.sc.mahidol.ac.th/scbt/aca...0Web/media.htm masterplain88

09-09-09, 20:08	#10 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	Try to stick to no more than 4%-5% sugar for good fungi agar. That is the only real "rule." And that isn't really a rule, more or less will work. Best agar recipe depends on what you are doing with it. Long term storage agar (in a slant or a petri) should be jacked up full of nutrition. An Amaranth/oatmeal/bee pollen/dog food provides a nice spectrum of nutes for storage. If you are just growing out cultures, testing samples or isolating - a basic LME or dextrose agar is your best choice. Agar for quick temporary growth should not have a lot of "extra" nutrients - those nutes could feed contams. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

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