Clone material directly to grain jars

[BigAlBlack]

just as the title says.

from everything that i've read and seen about cloning i've never seen this to be an advisable technique, and why idk because it works fuggin fantastic!

so if you want to clone and don't wanna use agar or a lc, just use a glovebox and put your cleaned clone tissue directly in your grain jar of choice, incubate and wait.

in just three days i've got more growth from this method than i ever did with an lc or agar.

[Myc]

Can you post a tek?

What size jars did you use?
I would love to see some pics
Never thought of this idea

[BigAlBlack]

im sure any jar size will work.

do work in a glovebox with sterile procedures.

take tissue from inner of fruit.

clean it in peroxide water solution (let it fizz for a few seconds)

open your grain jar and drop it in, close the jar back up.

incubate. wait.

sorry crappy pics, but it's all i've got. this is three days after transfer. and i used a thin strip of tissue about a half inch long spawned to popcorn/verm half pints. this is just the new growth from that piece of tissue. with the naked eye it's easy to see the strands coming from the original piece i used. this is the best of 4 jars after just 3 days, though all the others are showing clean new growth as well. on agar it has taken me 5-7 days to see any new growth whatsoever. this has been much quicker for me. i wouldn't do it without a glovebox though.

[BuckarooBanzai]

Popcorn is great for growing out clones and testing syringes/LCs.

Nutrition is very complete, growth is fast and the space between the kernels makes it SUPER easy to spot contams growing instead of myc.

AND after you fill a popcorn pint with myc, it is super easy to inject some sterile water, shake and extract a slurry syringe.

Rye grains are too small to beat the myc up enough to make a good slurry.

[Moose]

Just recently I took as freshly picked mushroom and as a joke tore it apart, ripped a piece of stem out and stuffed it into a pre-sterilized jar of Hpoo/Verm just using my hands and absolutely no sterile procedure. To my surprise the jar colonized in 2 weeks without any contams! It's on flush two now and still going strong. Not really a procedure I would recommend, but certainly shows just how resilient mycelium can be. Strain was B+.

Moose

[suckerfree]

Originally Posted by BigAlBlack

just as the title says.

from everything that i've read and seen about cloning i've never seen this to be an advisable technique, and why idk because it works fuggin fantastic!

so if you want to clone and don't wanna use agar or a lc, just use a glovebox and put your cleaned clone tissue directly in your grain jar of choice, incubate and wait.

in just three days i've got more growth from this method than i ever did with an lc or agar.

yup i do this, i'd recommend not using 'full' grain jars, maybe an inch or two if using quarts, or half pints would work well... (i get more contams using this cloning method, use less corn waste less corn same results.)

[rapture99]

why do u clean it in peroxide water solution is that ok for it cant u just put a piece of clean flesh in and it will work

[BigAlBlack]

Originally Posted by rapture99

why do u clean it in peroxide water solution is that ok for it cant u just put a piece of clean flesh in and it will work

to minimize the chance of contams. always been standard procedure for me, no matter what tek used.

[BigAlBlack]

Originally Posted by suckerfree

yup i do this, i'd recommend not using 'full' grain jars, maybe an inch or two if using quarts, or half pints would work well... (i get more contams using this cloning method, use less corn waste less corn same results.)

im fairly confident in my glovebox procedures is why i use a full jar. contams are scarce round here.

but yeah if you were worried that you might have contams i'd go small, or even not try at all. i myself wouldn't chance it without the use of a glovebox. but that's just me. really a glovebox is all around standard procedure for any fungi work for me. it's too simple with a proven track record without having to buy/construct a flow hood. i don't want to go back to breeding monster contams and fighting them for grows as is what i did when i started without using a glovebox. once they are introduced they are a biotch to get rid of completely.

and moose, that's amazing. got some pics of that grow?

[Zozo]

Originally Posted by BuckarooBanzai

Popcorn is great for growing out clones and testing syringes/LCs.

Nutrition is very complete, growth is fast and the space between the kernels makes it SUPER easy to spot contams growing instead of myc.

AND after you fill a popcorn pint with myc, it is super easy to inject some sterile water, shake and extract a slurry syringe.

Rye grains are too small to beat the myc up enough to make a good slurry.

I will highly recommend this method

thanks buckaroo banzai
peace
zozo

[rapture99]

Thanks for the answer BigAlBlack .Does the peroxide slow down or damage anything

[MLBjammer]

Mycelium can survive peroxide, but I would only use it if contams are an issue. A piece of interior tissue in a clean GB should be sterile, so there would be no need for a peroxide bath.