super easy way to make lots of spawn

[losfreddy]

Take some left over spawn from a colonized jar; dont need much. Pasturalize some horse poo, Stuff it in a lemonade bottle (with maybe 2 small polyfilled holes), mix with spawn and set on window sill.. Once fully colonized, cut open bottle and break up as if it came out of PF jar and use to case.. Save some and repeat with new bottle.

This has worked very well for me and you can just keep repeating. Awsome with aggressive strains.. Just thought i'd share

[Kreat]

Nice idea, how do you get it out of the bottle?

[MLBjammer]

Cut the bottle open, he says.

Question for you, Freddy: Why do you put the bottle in a window?

[weeeeeee]

Originally Posted by losfreddy

.................
This has worked very well for me and you can just keep repeating. Awsome with aggressive strains.. Just thought i'd share

just curious how many generations old is your dicaryotic myc i usually try to restart every few generations to keep the myc healthy though i do admit the older gens seem to show improved contam resistance they just dont seem to fruit as well i get alot more aborts. i noticed this from doing g2g's a little too far back without using a master jar...

not sure if this all true just my observations id like to hear some feedback on it though as my lazy ass has never tried to hit up chat.....

[TVCasualty]

Originally Posted by weeeeeee

not sure if this all true just my observations id like to hear some feedback on it though as my lazy ass has never tried to hit up chat.....

Yup, it's true. It's called senescence and other members have reported it becoming a problem after too many generations of taking clones from clones. There are a finite number of cell divisions possible for a given phenotype before it will be seen to weaken and ultimately won't fruit.

[TVCasualty]

Originally Posted by losfreddy

Take some left over spawn from a colonized jar; dont need much. Pasturalize some horse poo, Stuff it in a lemonade bottle (with maybe 2 small polyfilled holes), mix with spawn and set on window sill.. Once fully colonized, cut open bottle and break up as if it came out of PF jar and use to case.. Save some and repeat with new bottle.

Cool, that's a lot like "super spawning." In fact, I guess it is super spawning on a smaller scale, which is sweet! Just renew your mycelial DNA every once in a while and it should keep working great.

[BuckarooBanzai]

I have found it VERY effective to mix a small amount (1/10 by volume) of coir:castings bulk sub to my popcorn spawn bags. I believe the myc gets a leg up on producing the enzymes it will need to consume the same coir:castings bulk sub later when it is spawned. And the bags run almost as quickly.

I forgot about that term super-spawning, TV. Good call from the memory cells, dude! I never would have remembered that...

A cup of coir:castings in a popcorn spawn bag also allows you to use more inoculant without over-wetting and getting pooling water. I like a LOT of inoculant. Those big 'ol spawn bags finish real quick like when you shoot 'em with 120mL of slurry and incubate at 85F!

All cultures will eventually slow down, even if you keep refrigerated masters. But a refrigerated grain master (that doesn't contaminate) can stay strong for many months and give rise to literally hundreds of pounds of mycelia.

[losfreddy]

Originally Posted by Kreat

Nice idea, how do you get it out of the bottle?

you have to cut it out. Not a biggie to me, cause i drink these all the time

Originally Posted by MLBjammer

Cut the bottle open, he says.

Question for you, Freddy: Why do you put the bottle in a window?

I put behind curtain on window sill so its out of direct sunlight and so people dont see it when they walk in my room

[losfreddy]

Originally Posted by weeeeeee

just curious how many generations old is your dicaryotic myc ...

It is my 2nd time to repeat, but strain is 2nd generation from wild .. Menace strain

[MLBjammer]

Okay, you are putting it in the window sill as a stealth move. Kewl!

[P4ulada]

Nice idea

[weeeeeee]

Originally Posted by TVCasualty

Yup, it's true. It's called senescence and other members have reported it becoming a problem after too many generations of taking clones from clones. There are a finite number of cell divisions possible for a given phenotype before it will be seen to weaken and ultimately won't fruit.

i like to think of it as like super inbreeding after about four gens it seems to get so dicaryatic that its sort of retarded

[BuckarooBanzai]

Cubensis maintains a biological clock and that clock is programmed to eventually run down and stop.

Ya have to hit the reset button and start from spores every so often.

Mycelia growth is much faster and more robust within the first few months of germination anyway.