In Vitro Liquid Culture

essentrik1 · 09-27-09, 22:21

Here is something really cool. I use a stir bar and stir plate with my liquid cultures. When the bar is stirring at full speed it raises the level of liquid a little. Well I had one that I had let sit for a couple days on the counter without the stir bar going. Being on the counter, it was exposed to daily sunlight. I looked at it tonight and this is what I saw; some little shroom babies growing where the liquid goes up to while stirring. It's hard to see in the pictures through the glass, but you can kind of see the outline in the second picture. I'm thinking about cloning the one of the little buggers. If they grew there they have to be pretty strong and resilient, not? It's also just kind of cool. Any thoughts on this?

The first picture is where the cluster with the biggest shrooms is, attached to the glass. The second picture is the same cluster from the other side of the jar. Sorry for the crappy quality. It's hard to photograph something that small through a layer or two of glass.

BGB · 09-27-09, 22:23

Very fucking cool! I have never seen anything like that before man. I bet if there was a way to oxygenate the liquid it might grow!

essentrik1 · 10-01-09, 00:01

Any thoughts on cloning it?

(neo)Figment · 10-01-09, 16:51

I can't really see it but fuck yeah thats cool.
I have seen pics of mushrooms invitro on agar plates in Stamets book before.
Very cool.

GadgetGuy · 10-01-09, 17:00

Clone it

If it's a substrain that can fruit in LC it's gotta be a good one.

essentrik1 · 10-02-09, 23:47

I just wanted to add that this is dixieland. I've read great things about it being a nice strain, but this is surprising. I'll have to see if I can get more, but better quality, pics. The mycelium is growing bigger. I think I may see some caps forming in a few different places now. Very, very small, but it's a darker spot. I'm thinking I'm going to have to open this up pretty soon to clone. I might close it back up and see how much it grows before it becomes contaminated or the growth starts rotting.

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09-27-09, 22:21	#1 (permalink)
essentrik1 Mycophiliac Join Date: Jan 2009 Posts: 54	In Vitro Liquid Culture Here is something really cool. I use a stir bar and stir plate with my liquid cultures. When the bar is stirring at full speed it raises the level of liquid a little. Well I had one that I had let sit for a couple days on the counter without the stir bar going. Being on the counter, it was exposed to daily sunlight. I looked at it tonight and this is what I saw; some little shroom babies growing where the liquid goes up to while stirring. It's hard to see in the pictures through the glass, but you can kind of see the outline in the second picture. I'm thinking about cloning the one of the little buggers. If they grew there they have to be pretty strong and resilient, not? It's also just kind of cool. Any thoughts on this? The first picture is where the cluster with the biggest shrooms is, attached to the glass. The second picture is the same cluster from the other side of the jar. Sorry for the crappy quality. It's hard to photograph something that small through a layer or two of glass. Attached Thumbnails

10-01-09, 16:51	#4 (permalink)
(neo)Figment Lets rot some poop! Join Date: Mar 2007 Posts: 323	I can't really see it but fuck yeah thats cool. I have seen pics of mushrooms invitro on agar plates in Stamets book before. Very cool. __________________ "If you don't like my fire then don't come around, cause I'm gonna burn one down."

10-01-09, 17:00	#5 (permalink)
GadgetGuy Stupid Snowboarder... Join Date: Feb 2009 Posts: 1,311	Clone it If it's a substrain that can fruit in LC it's gotta be a good one. __________________ I have to hold on to the grass or I'll fall off the world!

10-02-09, 23:47	#6 (permalink)
essentrik1 Mycophiliac Join Date: Jan 2009 Posts: 54	I just wanted to add that this is dixieland. I've read great things about it being a nice strain, but this is surprising. I'll have to see if I can get more, but better quality, pics. The mycelium is growing bigger. I think I may see some caps forming in a few different places now. Very, very small, but it's a darker spot. I'm thinking I'm going to have to open this up pretty soon to clone. I might close it back up and see how much it grows before it becomes contaminated or the growth starts rotting. Last edited by essentrik1; 10-02-09 at 23:47. Reason: typo

09-27-09, 22:23	#2 (permalink)
BGB the one who ate too many. Join Date: Sep 2009 Posts: 250	Very fucking cool! I have never seen anything like that before man. I bet if there was a way to oxygenate the liquid it might grow!

10-01-09, 00:01	#3 (permalink)
essentrik1 Mycophiliac Join Date: Jan 2009 Posts: 54	Any thoughts on cloning it?

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