Cloning S.A.from fruit to LC

RedOctober · 09-27-09, 22:50

I've been trying to clone a fruit of the South American strain. I have had tremendous difficulties doing liquid culture with this strain. It just doesn’t want to grow. Someone finally suggested the 1tp karo, 1tsp honey, 500mL water recipe, which was the only thing that worked for germinating the S.A. spores. Unfortunately this hasn’t worked for cloning. The tissue seems to just die when I put it in to the liquid and it never grows. Anyone out there who has cloned S.A. tissue with a liquid culture, what was your recipe for success? This is really making me nuts!

TheFreak · 09-28-09, 00:30

what type of lids u using?do

do you give it fresh air exchange? every culture needs one at least once every 2 weeks in my experience.

apokalypse · 09-28-09, 03:49

A couple questions..

1) Are you PCing your LCs?
2) Do other strains germinate in them?
3) Are you using a sterile knife to extract a piece of the mushroom to clone?
4) Where are you extracting said piece from, the stalk?

BGB · 09-28-09, 10:37

I would just follow 9er cloning tek and shoot up jars directly with that. It works great imo.

RedOctober · 09-28-09, 16:37

The lids I am using are regular lids with silicone airports but no gas exchange. In the past with other strains I've found that they don't really need it and swirling the LC around once a day is sufficient, but I could be mistaken. Perhaps you are right Freak. I made this mistake with logs and giving them fresh air made all the difference in the world.

I do PC my LC's and other strains seem to do fine, I've tried GT and cambodian. Don't know what S.A.'s problem is!

My blade is sterile as I don't unpackage it until it's in the glove box, and I am extracting from the stem.

Moose · 09-28-09, 23:53

How long has the cloned tissue LC's been incubating? What is the temp of the incubator? I've cloned tissue and waited upwards of 3 weeks to see growth. Thought the tissue was dead, but eventually it did grow and made a real nice LC, just took forever.

Moose

RedOctober · 09-29-09, 16:23

Interesting Moose, maybe I'm too impatient. The spores were first germinated in a LC for aprox 2-2.5 weeks, then inoculated quart jars which were done in about 10 days, then spawned logs which again colonized and birthed in about 10 days and have been fruiting for the last 3 or so weeks. So all in all I'd say 70+ days or so. Incubated at 81 and fruited at 79.

P.S. I will try the 9-er tek. And I just read about the shmuvbox, that thing looks awesome! The whole glovebox thing is just annoying and building or obtaining a flowhood is expensive and difficult.

Moose · 09-29-09, 20:59

Your incubating temp is good but IMO your fruiting temp is a little high, I usually fruit at 74-75F and incubate at about 83F. I don't really swirl my LC's though, just put a marble into the jar and rock it for 10 seconds once a week. My guess is that S.A. may perhaps just be a stubborn strain to clone.

Moose

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09-27-09, 22:50	#1 (permalink)
RedOctober Mycophage Join Date: Oct 2005 Posts: 193	Cloning S.A.from fruit to LC I've been trying to clone a fruit of the South American strain. I have had tremendous difficulties doing liquid culture with this strain. It just doesn’t want to grow. Someone finally suggested the 1tp karo, 1tsp honey, 500mL water recipe, which was the only thing that worked for germinating the S.A. spores. Unfortunately this hasn’t worked for cloning. The tissue seems to just die when I put it in to the liquid and it never grows. Anyone out there who has cloned S.A. tissue with a liquid culture, what was your recipe for success? This is really making me nuts!

09-28-09, 03:49	#3 (permalink)
apokalypse Dr. Durgs Join Date: Apr 2005 Posts: 1,456	A couple questions.. 1) Are you PCing your LCs? 2) Do other strains germinate in them? 3) Are you using a sterile knife to extract a piece of the mushroom to clone? 4) Where are you extracting said piece from, the stalk? __________________ "Soo, babies don't come out the butt?"

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09-28-09, 00:30	#2 (permalink)
TheFreak Mycophage Join Date: Aug 2009 Posts: 139	what type of lids u using?do do you give it fresh air exchange? every culture needs one at least once every 2 weeks in my experience.

09-28-09, 10:37	#4 (permalink)
BGB the one who ate too many. Join Date: Sep 2009 Posts: 250	I would just follow 9er cloning tek and shoot up jars directly with that. It works great imo.

09-28-09, 16:37	#5 (permalink)
RedOctober Mycophage Join Date: Oct 2005 Posts: 193	The lids I am using are regular lids with silicone airports but no gas exchange. In the past with other strains I've found that they don't really need it and swirling the LC around once a day is sufficient, but I could be mistaken. Perhaps you are right Freak. I made this mistake with logs and giving them fresh air made all the difference in the world. I do PC my LC's and other strains seem to do fine, I've tried GT and cambodian. Don't know what S.A.'s problem is! My blade is sterile as I don't unpackage it until it's in the glove box, and I am extracting from the stem.

09-28-09, 23:53	#6 (permalink)
Moose Big Game Shroomer Join Date: Jun 2009 Posts: 140	How long has the cloned tissue LC's been incubating? What is the temp of the incubator? I've cloned tissue and waited upwards of 3 weeks to see growth. Thought the tissue was dead, but eventually it did grow and made a real nice LC, just took forever. Moose

09-29-09, 16:23	#7 (permalink)
RedOctober Mycophage Join Date: Oct 2005 Posts: 193	Interesting Moose, maybe I'm too impatient. The spores were first germinated in a LC for aprox 2-2.5 weeks, then inoculated quart jars which were done in about 10 days, then spawned logs which again colonized and birthed in about 10 days and have been fruiting for the last 3 or so weeks. So all in all I'd say 70+ days or so. Incubated at 81 and fruited at 79. P.S. I will try the 9-er tek. And I just read about the shmuvbox, that thing looks awesome! The whole glovebox thing is just annoying and building or obtaining a flowhood is expensive and difficult.

09-29-09, 20:59	#8 (permalink)
Moose Big Game Shroomer Join Date: Jun 2009 Posts: 140	Your incubating temp is good but IMO your fruiting temp is a little high, I usually fruit at 74-75F and incubate at about 83F. I don't really swirl my LC's though, just put a marble into the jar and rock it for 10 seconds once a week. My guess is that S.A. may perhaps just be a stubborn strain to clone. Moose