How long to PC pint BRF jars?

Rooster · 09-29-09, 20:03

So I have all kinds of LC around here and havnt had any luck with WBS spawn(I know my problem, I've been leaving it too wet). I'd rather not use it anyways and just use BRF jars instead. I wanna try using pint jars instead of the usual half pint and see how things work out. I'm hoping things work out well since I will be using LC instead of spores. I have the jars in the PC right now, how long would you guys recommend I have them in there?

hyphaenation · 09-29-09, 20:08

Between 45 minutes and 60 minutes at 15 PSI will do it.

Rooster · 09-29-09, 20:09

Thats exactly what I was thinking, I just didnt say anything for some reason. Thank you.

GadgetGuy · 09-29-09, 20:13

I've gotten away with 30 but I usually go 45 minutes for full pints. I like full pints

shroom57 · 09-29-09, 20:45

I like to go 60 minutes on pints...

Also, keep in mind that you need to strive to get enough LC in each hole to run down the side toward the bottom of the jar. The closer to the bottom you can initiate growth the faster the pint will colonize and help prevent any problems with stalled growth or contaminants.

GadgetGuy · 09-29-09, 21:06

Quote:

Originally Posted by shroom57

I like to go 60 minutes on pints...

Also, keep in mind that you need to strive to get enough LC in each hole to run down the side toward the bottom of the jar. The closer to the bottom you can initiate growth the faster the pint will colonize and help prevent any problems with stalled growth or contaminants.

Agreed. I douse my full pints with LC. I never check really but I do 4 holes with 1/4cc or more each hole and let it run to the bottom of the jars. Full pints colonize really fast that way.

BuckarooBanzai · 09-29-09, 21:44

30 minutes can work with cakes. 60 is safer and what I do.

The only problem with pint cakes can be how long they take to colonize. As mentioned above, use plenty of LC. You could easily get away with a full 1mL per inoculation hole - probably 1.5mL.

What temp will you be using for incubation?

Rooster · 11-04-09, 04:39

Just went and picked me up another 60 pint jars. Got em filled with BRF and PC'ing them now. What temp should the jars be incubated at? And what temp should I incubate my monotubs at during colonization? I havnt really had to worry about incubating things until recently since the weather is chilly now.

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09-29-09, 20:03	#1 (permalink)
Rooster Mycophiliac Join Date: Jul 2009 Posts: 86	How long to PC pint BRF jars? So I have all kinds of LC around here and havnt had any luck with WBS spawn(I know my problem, I've been leaving it too wet). I'd rather not use it anyways and just use BRF jars instead. I wanna try using pint jars instead of the usual half pint and see how things work out. I'm hoping things work out well since I will be using LC instead of spores. I have the jars in the PC right now, how long would you guys recommend I have them in there?

09-29-09, 20:08	#2 (permalink)
hyphaenation Puck Teknician Join Date: Feb 2007 Posts: 5,646	Between 45 minutes and 60 minutes at 15 PSI will do it. __________________ We're just flying by the seed of our plants.

09-29-09, 20:13	#4 (permalink)
GadgetGuy Stupid Snowboarder... Join Date: Feb 2009 Posts: 1,307	I've gotten away with 30 but I usually go 45 minutes for full pints. I like full pints __________________ I have to hold on to the grass or I'll fall off the world!

09-29-09, 20:45	#5 (permalink)
shroom57 Papa Smurf Join Date: Jul 2007 Posts: 293	I like to go 60 minutes on pints... Also, keep in mind that you need to strive to get enough LC in each hole to run down the side toward the bottom of the jar. The closer to the bottom you can initiate growth the faster the pint will colonize and help prevent any problems with stalled growth or contaminants. __________________ If the doors of perception were cleansed, every thing would appear to man as it is, infinite - William Blake

09-29-09, 20:09	#3 (permalink)
Rooster Mycophiliac Join Date: Jul 2009 Posts: 86	Thats exactly what I was thinking, I just didnt say anything for some reason. Thank you.

09-29-09, 21:44	#7 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	30 minutes can work with cakes. 60 is safer and what I do. The only problem with pint cakes can be how long they take to colonize. As mentioned above, use plenty of LC. You could easily get away with a full 1mL per inoculation hole - probably 1.5mL. What temp will you be using for incubation? __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

11-04-09, 04:39	#8 (permalink)
Rooster Mycophiliac Join Date: Jul 2009 Posts: 86	Just went and picked me up another 60 pint jars. Got em filled with BRF and PC'ing them now. What temp should the jars be incubated at? And what temp should I incubate my monotubs at during colonization? I havnt really had to worry about incubating things until recently since the weather is chilly now.

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