The Little Culture That Could

Myc · 10-04-09, 14:41

I've seen a few threads relative to this research.

I don't recommend it as a course of study. You never know what you might breed and release into the lab if you're not careful.

The cultivar I've been working with was a rescue effort started about a year ago or more.

MS innoculated to agar.
Agar transfer to rye berries.
During the course of colonization, the jar became extremely contaminated with multiple species of mold.
I wanted to see if the living culture could hold its own.

After several months of observation, a single 'island' of myc survived. This culture was surrounded mind you by mold - not a single un-colonized kernel in that jar.
As if in a last act of defiance, that 'island' produced a pin.

This little island was rescued and returned to agar for cleanup.
The original plate is on the Left in the photos.
Second generation transfer plate on the Right.

As you can see, gent. agar is not always the magic bullet.
Contaminants persisted.......but so did I.

And the little culture that could has rewarded my efforts today!

All of this was made possible through study here at 'Topia and the Bonzai Institute of Micropropagation.

kcmoxtractor · 10-04-09, 14:51

This is the semperviva?

buteo · 10-04-09, 14:55

nice inspiration of hope there. when the world gets rough and your down to your last breath....just remember the culture that could that made it through the "impossible".

BuckarooBanzai · 10-04-09, 15:29

There ya go, man! Life finds a way.

With a little agar and a little careful technique, you can clean up any culture.

I have successfully grown out dried bag shrooms and 3 year old dried stem tissue AFTER cleaning them up.

Great threadage, man.

door#6 · 10-04-09, 22:44

nice... contam resistant strains anyone?

Dr.Hallucination. · 10-04-09, 23:24

Very nice work Myc,I can't wait to see these when there full grown.

Myc · 10-05-09, 09:30

The culture is maturing nicely.
I'll be watching these closely.

Even though it's in a monotub setup, fanning is necessary.
The culture is infected again - and is giving off detectable levels of ammonia (as if it were in the beginning stages of composting).

The primordia/hyphael knots look EXACTLY like trichoderma.
Brilliant white - dusty and crumbly looking at first.

I have several of these projects going on various experimental substrates.

I've been picking the large patches of primordia off of some of the other projects - thinking it was the early onset of trich!!!

As the projects mature, they develop a web-like formation on the surface. Very reminiscent of Cobweb. In fact, I increased FAE as a result of this 'cobweb' formation.
Come to find out.....
This is just a surface mycelial network from which the hyphael knotting begins!!

I'll need to watch these from start to finish.
Due to the possibility that somthing other than what is expected may be maturing.

Om shanti · 10-05-09, 10:01

This is exciting.

MLBjammer · 10-05-09, 11:40

I would bet that the mycelium will conquer. This thread is fascinating. Thanks, Myc.

mushmind · 10-05-09, 13:27

i've seen myc win out before.

Myc · 10-06-09, 09:45

Looks like I have an invader of a higher order in my tub.

I'm guessing from the genre coprinaceae

Maybe I'll get lucky and it will be coprinus comatus!!

Although a little let down, this is still a win!
I get to study and observe a fungus unfamiliar to me.

BuckarooBanzai · 10-06-09, 11:10

That's not an infection, it's full on invasion and occupation!

Pretty cool!

Myc · 10-06-09, 22:40

I must be lucky or something....
Even my failures seem to result in a win sometimes.

I'm having a ball watching these.
Can't wait for sporulation. Microscopy to follow.

kcmoxtractor · 10-06-09, 22:42

Wow, I don't think I have seen this happen before.

Do you think the invader was there upon spawning or came around during the spawn run?

BuckarooBanzai · 10-06-09, 23:09

Myc, you are a friend, but I must be honest with you about something.

You are, I am afraid to say, afflicted by the blue thumb.

I'm sorry, man...

Myc · 10-07-09, 10:30

This has been among my most exciting projects.
I should be able to do microscopy right away with these since the spore deposit is pretty 'moist' or wet for lack of a better description.

I'm still sticking with my tentative ID of
Coprinus comatus
Any other guesses?

Myc · 10-07-09, 11:28

I've changed my guess to
Coprinopsis lagopus

Om shanti · 10-07-09, 11:36

LOL, very cool!!!
Did you also do microscopy of any molds, by the way?

Myc · 10-07-09, 12:02

No mold microscopy as of yet. LOL

I'm still scratching my head wondering where this came from.
I'm reminded of a Beatles song "...she came in through the bathroom window..."

These were raised from MS most definately
The print came from a lab specimen from a known - solid mycologist

I guess I must be getting addled. Maybe I confused this project with a semilanceata or hispanica project. Both of those projects are from wild prints and it is conceivable that I've been fostering a competitor spore for all this time.
The cultivar appears consistently in all experiments
Which tells me that I have this species on a plate as a presumed "master culture" - and mislabled.

I'm laughing my ass off at myself
This is a freakin' hoot!!!

I LOVE mycology!

Fully colonized spawnbags available for the asking

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10-04-09, 14:41	#1 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 2,041	The Little Culture That Could I've seen a few threads relative to this research. I don't recommend it as a course of study. You never know what you might breed and release into the lab if you're not careful. The cultivar I've been working with was a rescue effort started about a year ago or more. MS innoculated to agar. Agar transfer to rye berries. During the course of colonization, the jar became extremely contaminated with multiple species of mold. I wanted to see if the living culture could hold its own. After several months of observation, a single 'island' of myc survived. This culture was surrounded mind you by mold - not a single un-colonized kernel in that jar. As if in a last act of defiance, that 'island' produced a pin. This little island was rescued and returned to agar for cleanup. The original plate is on the Left in the photos. Second generation transfer plate on the Right. As you can see, gent. agar is not always the magic bullet. Contaminants persisted.......but so did I. And the little culture that could has rewarded my efforts today! All of this was made possible through study here at 'Topia and the Bonzai Institute of Micropropagation. __________________ "...we'd like to help you learn to help yourself..."

10-04-09, 14:51	#2 (permalink)
kcmoxtractor Satan's Helper Join Date: Jun 2008 Posts: 1,349	This is the semperviva? __________________ "It was the straying that found the path direct" - Austin Osman Spare

10-04-09, 14:55	#3 (permalink)
buteo Imbicilzzzz Join Date: Oct 2008 Posts: 1,355	nice inspiration of hope there. when the world gets rough and your down to your last breath....just remember the culture that could that made it through the "impossible". __________________ Im only what i think i am

10-04-09, 15:29	#4 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	There ya go, man! Life finds a way. With a little agar and a little careful technique, you can clean up any culture. I have successfully grown out dried bag shrooms and 3 year old dried stem tissue AFTER cleaning them up. Great threadage, man. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

10-04-09, 22:44	#5 (permalink)
door#6 one billion served Join Date: Feb 2008 Posts: 62	nice... contam resistant strains anyone?

10-04-09, 23:24	#6 (permalink)
Dr.Hallucination. Chasing the Rabbit. Join Date: Jan 2007 Posts: 1,820	Very nice work Myc,I can't wait to see these when there full grown. __________________ The man who walk's through the door on the wall,Will never be quite the same,As the man who went out.

10-05-09, 09:30	#7 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 2,041	The culture is maturing nicely. I'll be watching these closely. Even though it's in a monotub setup, fanning is necessary. The culture is infected again - and is giving off detectable levels of ammonia (as if it were in the beginning stages of composting). The primordia/hyphael knots look EXACTLY like trichoderma. Brilliant white - dusty and crumbly looking at first. I have several of these projects going on various experimental substrates. I've been picking the large patches of primordia off of some of the other projects - thinking it was the early onset of trich!!! As the projects mature, they develop a web-like formation on the surface. Very reminiscent of Cobweb. In fact, I increased FAE as a result of this 'cobweb' formation. Come to find out..... This is just a surface mycelial network from which the hyphael knotting begins!! I'll need to watch these from start to finish. Due to the possibility that somthing other than what is expected may be maturing. Attached Thumbnails __________________ "...we'd like to help you learn to help yourself..."

10-05-09, 10:01	#8 (permalink)
Om shanti S.W.I.M. in H.POO Join Date: Jan 2008 Posts: 1,297	This is exciting. __________________ The most important thing is to find out what is the most important thing.-S. Suzuki

10-05-09, 11:40	#9 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 1,864	I would bet that the mycelium will conquer. This thread is fascinating. Thanks, Myc.

10-05-09, 13:27	#10 (permalink)
mushmind campus radical Join Date: Sep 2008 Posts: 299	i've seen myc win out before. __________________ democracy is not a spectator sport! so get out there and be heard!

10-06-09, 09:45	#11 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 2,041	I'm glad I didn't ID these yet Looks like I have an invader of a higher order in my tub. I'm guessing from the genre coprinaceae Maybe I'll get lucky and it will be coprinus comatus!! Although a little let down, this is still a win! I get to study and observe a fungus unfamiliar to me. Attached Thumbnails __________________ "...we'd like to help you learn to help yourself..."

10-06-09, 11:10	#12 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	That's not an infection, it's full on invasion and occupation! Pretty cool! __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

10-06-09, 22:40	#13 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 2,041	I must be lucky or something.... Even my failures seem to result in a win sometimes. I'm having a ball watching these. Can't wait for sporulation. Microscopy to follow. Attached Thumbnails __________________ "...we'd like to help you learn to help yourself..."

10-06-09, 22:42	#14 (permalink)
kcmoxtractor Satan's Helper Join Date: Jun 2008 Posts: 1,349	Wow, I don't think I have seen this happen before. Do you think the invader was there upon spawning or came around during the spawn run? __________________ "It was the straying that found the path direct" - Austin Osman Spare

10-06-09, 23:09	#15 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,050	Myc, you are a friend, but I must be honest with you about something. You are, I am afraid to say, afflicted by the blue thumb. I'm sorry, man... __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

10-07-09, 10:30	#16 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 2,041	This has been among my most exciting projects. I should be able to do microscopy right away with these since the spore deposit is pretty 'moist' or wet for lack of a better description. I'm still sticking with my tentative ID of Coprinus comatus Any other guesses? Attached Thumbnails __________________ "...we'd like to help you learn to help yourself..."

10-07-09, 11:28	#17 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 2,041	I've changed my guess to Coprinopsis lagopus Attached Thumbnails __________________ "...we'd like to help you learn to help yourself..."

10-07-09, 11:36	#18 (permalink)
Om shanti S.W.I.M. in H.POO Join Date: Jan 2008 Posts: 1,297	LOL, very cool!!! Did you also do microscopy of any molds, by the way? __________________ The most important thing is to find out what is the most important thing.-S. Suzuki

10-07-09, 12:02	#19 (permalink)
Myc El Jardinero Join Date: Apr 2007 Posts: 2,041	No mold microscopy as of yet. LOL I'm still scratching my head wondering where this came from. I'm reminded of a Beatles song "...she came in through the bathroom window..." These were raised from MS most definately The print came from a lab specimen from a known - solid mycologist I guess I must be getting addled. Maybe I confused this project with a semilanceata or hispanica project. Both of those projects are from wild prints and it is conceivable that I've been fostering a competitor spore for all this time. The cultivar appears consistently in all experiments Which tells me that I have this species on a plate as a presumed "master culture" - and mislabled. I'm laughing my ass off at myself This is a freakin' hoot!!! I LOVE mycology! Fully colonized spawnbags available for the asking __________________ "...we'd like to help you learn to help yourself..."

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