Invitro questions [merged]

Beshroomed · 07-23-06, 21:28

Hey,
Do PF jars that are exposed to indirect sunlight from innoculation on colonize slower that jars that are incubated in total darkness? A FOAF is trying the Invitro method for the first time and does not see any growth at 82 hours after innoculation. The jars were innoculated with PF classic spores with a spore syringe from Ralphs. The jars,(which were ordered from Mycrotopia), have been kept at 77 to 80 degrees except for 2 hours a day out of the incubation chamber for light exposure but the room temp is about 74 or 75 so that shouldn't matter I don't think.

robman1 · 07-23-06, 22:02

Fastest colonization is done at 86 degrees (at least so says most of the teks) and signs of growth are seen between 3-5 days.

You still have about another day or two until you can start worrying. Also the temps are a little low, so that might be slowing it down.

prismaticEffects · 07-23-06, 22:04

my friends temps werent in the ideal range,
growth was slow w/ no signs of mycelium until about day 10 i think.
i wouldn't worry yet anyway.

nomoreusmc · 07-23-06, 22:58

82 Hours? I wouldn't even look at them for two weeks!!

nomoreusmc · 07-23-06, 23:01

I say this expecting that you did muti-spore noc, not LC. IMO light doesn't make that big of diffrence. Look at it this way, when you plant a seed, you don't walk over to the pot three days later and expect a sprout.

~Hippie3 · 07-23-06, 23:13

light does not affect growth
other than to trigger pinning

Beshroomed · 08-09-06, 21:02

Hey,
A FOAF is trying the Hippie's Invitro tek for her first grow. It is day 20 now and the 1/2 pt PF style jars look 100% colonized. The jars have been turned upside down to rest on their lids for a few days now and she told me that there seems to be alot of water droplets forming on the inside of the jars. Is this normal?

Also, shouldn't pins be starting to show up pretty soon now?

Many thanks to everyone for all the great advice so far!

boots420 · 08-09-06, 21:20

never done invitro. but water droplets forming down the sides of jar is normal. if they are at day 20 and just finished looking like a 100%. then they are probably close to 100 an more like 95. give it sum time and pin they will.

chimp · 08-09-06, 21:25

Loosen the lids and caps a little if they are 100% colonized. Fresh air is one of the things that trigger pinning

~Hippie3 · 08-09-06, 21:43

once colonized you can initiate air exchanges.
just give bright light
and soon you'll see pinning

Beshroomed · 08-10-06, 17:44

Hi,
Does anyone think you should dunk before first flush when growing Invitro? Seems like I've read that some do and some don't. My FOAF is on day 21, opened jars for air exchange and shook off exceess verm, put back into jars with lids loose and sat jars on their lids. Everything looks healthy so far!
Hippie's jars are Great!!!
Thanks Hip, and everybody.

dial8 · 08-11-06, 08:00

Personally I would not. But people differ.

Dank Side Of The Shroom · 08-11-06, 08:16

i never have unless for some reason it takes a long ass time to colonize and im using it for spawn. other wise for cakes i do a 12 hr dunk after the first flush and a 24 hr dunk for 3rd flush on.

~Hippie3 · 08-11-06, 08:30

invitro cakes generally don't need a dunk
until after 1st flush

Invitro · 08-11-06, 08:59

Yes what Hip said, but you can remove the dry verm layer
once 100% colonized to make more headroom and better
air exchange. You can wait til you see the first pins before
removing the dry verm layer.

eternalfrost · 08-11-06, 09:10

my foaf has done it both ways with no problems.

deffinatly dump out the dry verm for space though. dont worry about picking off the verm that the mycelium has semi grown over. it ends up acting somewhat as a 'caseing' and he noticed alot more fruits growing out of the verm then elsewhere on the cake

Dank Side Of The Shroom · 08-11-06, 09:27

my foaf lets the first flush go in the 1/2 pint jars they started in and after the dunk she switches them over to 1 pint jars for the extra room. she said she dunked a couple befor the first flush and she got contams, but that is most likley her fault. not saying yuo will get them. just dunk 1 or 2 and compare them to the others for a little experment.

MLBjammer · 08-11-06, 10:09

Unless the cakes take like five weeks to colonize, I really would not dunk until after your first harvest. Like the wise ones above said, shake off the loose vermiculite and fan them every few days and get rid of any standing water in the jars. It is that simple. Peace.

Beshroomed · 08-13-06, 22:37

Can someone give some approximate time frames from innoculation until pinning starts? A FOAF say's cakes are fully colonized and have been kept at around 75 degrees, look real healthy, rhizo growth, water condensation on inside of jars looks good and air exchanges given every couple of days. She said that she thought she saw the beginnings of some pins a week ago when jars were first opened for air exchange and excess verm was shook off but nothing since.
Thanks all

Beshroomed · 08-13-06, 22:50

Oops, meant to post that in the fungi forum. Hope some nice moderator will move it for me.
Thanks

nomoreusmc · 08-14-06, 00:06

Quote:

Originally Posted by beeshrm

Can someone give some approximate time frames from innoculation until pinning starts? A FOAF say's cakes are fully colonized and have been kept at around 75 degrees, look real healthy, rhizo growth, water condensation on inside of jars looks good and air exchanges given every couple of days. She said that she thought she saw the beginnings of some pins a week ago when jars were first opened for air exchange and excess verm was shook off but nothing since.
Thanks all

Mushies have their own time table, trying to reliable predict their movements are infuriating, They will pin when they are darn good and ready! I have tried to predict all sorts of myco projects and it's all a crap shoot!!!

SnakeMan · 10-11-06, 16:57

if jars are placed in a closet and the temp is fluxn from like 68-77 is it going to hurt colonization. jars were innoc yesterday. if so then how to control temp if closet goes from 68 to 77 throughout the day

explorerowid · 10-11-06, 17:00

You should be just fine with those temps.
It may slow colonozation down a bit ,
but nothing to worry about .
good luck
E.E.

SnakeMan · 10-11-06, 17:14

Thats what i was thinking, but if the opt temp is 70 then wouldnt 77 speed things up?

sandman · 10-11-06, 17:24

a little bit

SnakeMan · 10-11-06, 19:11

thanks I cant wait for the myc to start growing

BuckarooBanzai · 10-11-06, 22:18

The best temp for incubation is not 70F. More like 80F-83F.

SnakeMan · 10-12-06, 11:12

what is a quick way to get the temp up? I was thinking about putting a blanket around the jars. So at 70 the colonization will be slower right?

dial8 · 10-12-06, 11:34

At 70 they will grow but slowly. Germination will be stalled a bit too. You can make a ver effective incubator for cheap. Try the TiT method. I'll try and find a link.

dial8 · 10-12-06, 11:39

Here is a pretty simple tek -http://mycotopia.net/discus/messages/5/3672.html?1004859042

~Hippie3 · 10-12-06, 11:59

another -
http://archives.mycotopia.net/discus...tml?1004778624

SnakeMan · 10-12-06, 16:23

so if i go with the pickle jar heater i can just place that in my closet? I know that a better way would be to put all 12 in a cont but the closet is pretty small and right now it is 77 degrees.

after doing some quick research i was thinking about getting a submersable heater place it in a pickle jar and place the jar in a rubbermaid cont with the jars. My question is, is that going to prodcue enough heat? I guess i can try it for a couple of days and if it dont work then ill the alien tek. or i could get a reptile heat pad and place it on top of the container. Thanks for all the help.

~Hippie3 · 10-12-06, 17:31

bigger jar, bigger heater = more heat

SnakeMan · 10-12-06, 20:00

so if i get a BIG jar and place a heater in there then it will in turn heat the closet enough to reach that 80-83 range?

~Hippie3 · 10-13-06, 07:13

only one way to find out...

SnakeMan · 10-17-06, 16:33

i ditched that idea and just built one...

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07-23-06, 21:28	#1 (permalink)
Beshroomed Mycophage Join Date: Jul 2006 Posts: 180	Invitro questions [merged] Hey, Do PF jars that are exposed to indirect sunlight from innoculation on colonize slower that jars that are incubated in total darkness? A FOAF is trying the Invitro method for the first time and does not see any growth at 82 hours after innoculation. The jars were innoculated with PF classic spores with a spore syringe from Ralphs. The jars,(which were ordered from Mycrotopia), have been kept at 77 to 80 degrees except for 2 hours a day out of the incubation chamber for light exposure but the room temp is about 74 or 75 so that shouldn't matter I don't think. __________________ Question Reality.

07-23-06, 22:04	#3 (permalink)
prismaticEffects Mycotopiate Join Date: May 2006 Posts: 434	my friends temps werent in the ideal range, growth was slow w/ no signs of mycelium until about day 10 i think. i wouldn't worry yet anyway. __________________ _____________________ _____________________ make my funk the p-funk. _____________________

07-23-06, 22:58	#4 (permalink)
nomoreusmc Mycotopiate Join Date: Sep 1971 Posts: 936	82 Hours? I wouldn't even look at them for two weeks!! __________________ Peace, Love and Shrooms

07-23-06, 23:01	#5 (permalink)
nomoreusmc Mycotopiate Join Date: Sep 1971 Posts: 936	I say this expecting that you did muti-spore noc, not LC. IMO light doesn't make that big of diffrence. Look at it this way, when you plant a seed, you don't walk over to the pot three days later and expect a sprout. __________________ Peace, Love and Shrooms

07-23-06, 22:02	#2 (permalink)
robman1 Mycophage Join Date: Jul 2006 Posts: 186	Fastest colonization is done at 86 degrees (at least so says most of the teks) and signs of growth are seen between 3-5 days. You still have about another day or two until you can start worrying. Also the temps are a little low, so that might be slowing it down.

07-23-06, 23:13	#6 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	light does not affect growth other than to trigger pinning

08-09-06, 21:02	#7 (permalink)
Beshroomed Mycophage Join Date: Jul 2006 Posts: 180	Invitro Question Hey, A FOAF is trying the Hippie's Invitro tek for her first grow. It is day 20 now and the 1/2 pt PF style jars look 100% colonized. The jars have been turned upside down to rest on their lids for a few days now and she told me that there seems to be alot of water droplets forming on the inside of the jars. Is this normal? Also, shouldn't pins be starting to show up pretty soon now? Many thanks to everyone for all the great advice so far! __________________ Question Reality.

08-09-06, 21:20	#8 (permalink)
boots420 We Get Lifted Join Date: Apr 2006 Posts: 585	never done invitro. but water droplets forming down the sides of jar is normal. if they are at day 20 and just finished looking like a 100%. then they are probably close to 100 an more like 95. give it sum time and pin they will.

08-09-06, 21:25	#9 (permalink)
chimp Space Lord Modulator Join Date: May 2006 Posts: 6,420	Loosen the lids and caps a little if they are 100% colonized. Fresh air is one of the things that trigger pinning __________________ Respond, Vibrate, Feedback, Resonate

08-09-06, 21:43	#10 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	once colonized you can initiate air exchanges. just give bright light and soon you'll see pinning

08-10-06, 17:44	#11 (permalink)
Beshroomed Mycophage Join Date: Jul 2006 Posts: 180	Invitro Dunking Question Hi, Does anyone think you should dunk before first flush when growing Invitro? Seems like I've read that some do and some don't. My FOAF is on day 21, opened jars for air exchange and shook off exceess verm, put back into jars with lids loose and sat jars on their lids. Everything looks healthy so far! Hippie's jars are Great!!! Thanks Hip, and everybody. __________________ Question Reality.

08-11-06, 08:00	#12 (permalink)
dial8 3 Join Date: Jun 1976 Posts: 4,420	Personally I would not. But people differ. __________________ For enlightenment please dial8.

08-11-06, 08:16	#13 (permalink)
Dank Side Of The Shroom Mycotopiate Join Date: Oct 2005 Posts: 3,515	i never have unless for some reason it takes a long ass time to colonize and im using it for spawn. other wise for cakes i do a 12 hr dunk after the first flush and a 24 hr dunk for 3rd flush on. __________________ [SIGPIC][/SIGPIC]

08-11-06, 08:30	#14 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	invitro cakes generally don't need a dunk until after 1st flush

10-11-06, 17:24	#25 (permalink)
sandman l̈́͂̏̔ͯ̒͒͊͒̃ͮ̍̍̚ȏ̾̂̉̍́ ͒͛́ Join Date: Apr 1972 Posts: 2,088	a little bit __________________ Dreams are real. But they are made of viewpoints, of images, of memories and puns and lost hopes

10-12-06, 11:39	#30 (permalink)
dial8 3 Join Date: Jun 1976 Posts: 4,420	Here is a pretty simple tek -http://mycotopia.net/discus/messages/5/3672.html?1004859042 Last edited by reverend trips; 10-22-06 at 19:37. Reason: fixed broken link

08-11-06, 08:59	#15 (permalink)
Invitro Don Vitro Join Date: May 2005 Posts: 314	Yes what Hip said, but you can remove the dry verm layer once 100% colonized to make more headroom and better air exchange. You can wait til you see the first pins before removing the dry verm layer.

08-11-06, 09:10	#16 (permalink)
eternalfrost Mycotopiate Join Date: May 2006 Posts: 812	my foaf has done it both ways with no problems. deffinatly dump out the dry verm for space though. dont worry about picking off the verm that the mycelium has semi grown over. it ends up acting somewhat as a 'caseing' and he noticed alot more fruits growing out of the verm then elsewhere on the cake __________________ No one knows what’s next, but everybody does it. Translucent Art - Exploring Beauty Through Computation

08-11-06, 09:27	#17 (permalink)
Dank Side Of The Shroom Mycotopiate Join Date: Oct 2005 Posts: 3,515	my foaf lets the first flush go in the 1/2 pint jars they started in and after the dunk she switches them over to 1 pint jars for the extra room. she said she dunked a couple befor the first flush and she got contams, but that is most likley her fault. not saying yuo will get them. just dunk 1 or 2 and compare them to the others for a little experment. __________________ [SIGPIC][/SIGPIC]

08-11-06, 10:09	#18 (permalink)
MLBjammer Addicted to Invitro Join Date: Mar 2006 Posts: 2,890	Unless the cakes take like five weeks to colonize, I really would not dunk until after your first harvest. Like the wise ones above said, shake off the loose vermiculite and fan them every few days and get rid of any standing water in the jars. It is that simple. Peace.

08-13-06, 22:37	#19 (permalink)
Beshroomed Mycophage Join Date: Jul 2006 Posts: 180	Invitro Question Can someone give some approximate time frames from innoculation until pinning starts? A FOAF say's cakes are fully colonized and have been kept at around 75 degrees, look real healthy, rhizo growth, water condensation on inside of jars looks good and air exchanges given every couple of days. She said that she thought she saw the beginnings of some pins a week ago when jars were first opened for air exchange and excess verm was shook off but nothing since. Thanks all __________________ Question Reality.

08-13-06, 22:50	#20 (permalink)
Beshroomed Mycophage Join Date: Jul 2006 Posts: 180	Oops, meant to post that in the fungi forum. Hope some nice moderator will move it for me. Thanks __________________ Question Reality.

10-11-06, 16:57	#22 (permalink)
SnakeMan Mycophiliac Join Date: Mar 2006 Posts: 76	Invitro questions [merged] if jars are placed in a closet and the temp is fluxn from like 68-77 is it going to hurt colonization. jars were innoc yesterday. if so then how to control temp if closet goes from 68 to 77 throughout the day

10-11-06, 17:00	#23 (permalink)
explorerowid Mycophage Join Date: Jun 2006 Posts: 102	You should be just fine with those temps. It may slow colonozation down a bit , but nothing to worry about . good luck E.E.

10-11-06, 17:14	#24 (permalink)
SnakeMan Mycophiliac Join Date: Mar 2006 Posts: 76	Thats what i was thinking, but if the opt temp is 70 then wouldnt 77 speed things up?

10-11-06, 19:11	#26 (permalink)
SnakeMan Mycophiliac Join Date: Mar 2006 Posts: 76	thanks I cant wait for the myc to start growing

10-11-06, 22:18	#27 (permalink)
BuckarooBanzai Prone to ranting... Join Date: Oct 2005 Posts: 6,053	The best temp for incubation is not 70F. More like 80F-83F. __________________ Banzai Institute for Higher Education (a collection of growing Teks & threads)

10-12-06, 11:12	#28 (permalink)
SnakeMan Mycophiliac Join Date: Mar 2006 Posts: 76	what is a quick way to get the temp up? I was thinking about putting a blanket around the jars. So at 70 the colonization will be slower right?

10-12-06, 11:34	#29 (permalink)
dial8 3 Join Date: Jun 1976 Posts: 4,420	At 70 they will grow but slowly. Germination will be stalled a bit too. You can make a ver effective incubator for cheap. Try the TiT method. I'll try and find a link.

10-12-06, 11:59	#31 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	another - http://archives.mycotopia.net/discus...tml?1004778624 Last edited by reverend trips; 10-22-06 at 19:35. Reason: fixed broken link

10-12-06, 16:23	#32 (permalink)
SnakeMan Mycophiliac Join Date: Mar 2006 Posts: 76	so if i go with the pickle jar heater i can just place that in my closet? I know that a better way would be to put all 12 in a cont but the closet is pretty small and right now it is 77 degrees. after doing some quick research i was thinking about getting a submersable heater place it in a pickle jar and place the jar in a rubbermaid cont with the jars. My question is, is that going to prodcue enough heat? I guess i can try it for a couple of days and if it dont work then ill the alien tek. or i could get a reptile heat pad and place it on top of the container. Thanks for all the help.

10-12-06, 17:31	#33 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	bigger jar, bigger heater = more heat

10-12-06, 20:00	#34 (permalink)
SnakeMan Mycophiliac Join Date: Mar 2006 Posts: 76	so if i get a BIG jar and place a heater in there then it will in turn heat the closet enough to reach that 80-83 range?

10-13-06, 07:13	#35 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,760	only one way to find out...

10-17-06, 16:33	#36 (permalink)
SnakeMan Mycophiliac Join Date: Mar 2006 Posts: 76	i ditched that idea and just built one...

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