invitro palenque on coir

[limeade]

what i'm working with at the moment,

i was suprised to see this much primordia, got some new cfls that have really improved my pinsets.

There seems to be some more work done, but wouldn't be hard, mostly the substrate is colonized with the same rhizomorphic mycelium, very few spots of a different tomentose mycelium present, going to be taking a print to agar on this one. Love the pinset forming!

Palenque on coir/verm/malt/dex/calc carb:

[livewire420]

looking good may the shroom gods be with you

[limeade]

Originally Posted by livewire420

looking good may the shroom gods be with you

Thanx, I was hoping this would do well, as i have a lot going on at one time, I'm also trying to get some albino penis envy going, and some penis envy, im retiring PESH, and starting Tasmanian, and PuertoRican, and going to open my slant of atlantis :P

So this was a good suprise to find something to work with that will preform well (fingers crossed)

[Hippie3]

hmm, looks a bit like trich, hope that stays white

[limeade]

Originally Posted by Hippie3

hmm, looks a bit like trich, hope that stays white

it's definitely not trichoderma, it's identifiably tomentose mycelium, the isolation wasn't quit finished, only 3 transfers. None of those plates have gone green. No grains have gone green.

But yeah, i thought it looked like trich for a minute when i noticed the different types of mycelium instead,

newb agar transer stuff.

[limeade]

getting ready to upload a few "macro" shots

[limeade]

Each bag looks like this , this evening, There is one close up, where u can see the two different types of mycelium, the second fluffier tomentose mycelium is starting to hyphally knot as of today, so yeah it's not trich! YAY.

I also scaled down the resolution, sorry about the large files.

My next question, is when will the pinset quit setting?
(never thought i'd complain about that!)

****as of sunday, i'll cut off light completely, there is no need in more pinning to be initiated, I have been killing lights after pinsets have been set for a while now, they tend to mature faster as far as i can tell, so LIGHTS OFF!

oh, and i would clone from a cluster, but it's all a cluster,

i assume we'll be seeing 2 distinct types of mushroom from the two different types of mycelium?

[limeade]

after 1 day of no light, maturation has begun. Again with no light today. (it was going to start anyhow though, im hoping no light will accelerate the process.

[livewire420]

man them pins are looking good. wish my pe would start pinning soon. think ill be trying the recipe out soon.

[limeade]

Originally Posted by livewire420

man them pins are looking good. wish my pe would start pinning soon. think ill be trying the recipe out soon.

hahah


I JUST posted my penis envy grow going right now, "Invitro Penis envy on Compost"

mine are out on 1st day to start pinning, just had to move them, as i'll not be using light in this area of the basement..

[Looky]

Originally Posted by limeade

****as of sunday, i'll cut off light completely, there is no need in more pinning to be initiated, I have been killing lights after pinsets have been set for a while now, they tend to mature faster as far as i can tell, so LIGHTS OFF!

That's a good observation. I thought that I noticed that too, but dismissed it. I thought that during my lights off part, there was a lot more growth than with the lights on.

[limeade]

Originally Posted by Lookyhere

That's a good observation. I thought that I noticed that too, but dismissed it. I thought that during my lights off part, there was a lot more growth than with the lights on.

the very first picture, in my last post of pictures, was the exact same bag, 23 hours ago, it sat in the dark 23 hours, and that popped up, the other pictures are of the same bag, all 14 look just like that though.

I cannot believe that's from a spore print, FROM a fruit, FROM an isolation.
and only 2 types of mycelium. That's strong genetics!

depending on potency, i may dedicate 5 plates to it.

about the lights off, i noticed it once when we had such terrible storms for 2 days, we had no electricity, I kept ice bombing by driving a county over and buying ice, to keep everything cool, with no light, i was harvesting and hanging drying baskets outside every 2 hours. It was fun!

When the electricity came back on, the lights came on, the fruits took longer on the bags that were behind, but coming up to maturity, they took 3 times as long. So i've been sticking with it.

Once a pinset is set, the dna is hardcoded with the information it needs from the light, at that point, no more light is needed, instead of giving it more light and forcing it to continually pin, reserve that energy for maturing what you've got!

[eatyualive]

looking good. you can definitely see the mycelial network differences when your using coir alone...

it kinda seems to make more pins but the strands spread out more. interesting looking. from those first pictures i was about to say they almost don't look completely colonized but yeah man looking good.


this happens sometimes in coir casing layers. looks just like that...

i did an experiment with tex strain using wild bird seed spawn and plain coir.

as you asked...the pinset never stopped setting. it continually flushes just randomely. it was like a never ending flush. i liked this result to a degree but the mushrooms didn't seem as solid or large as usually. it seemed the spawn ratio needed to be higher to get the results needed. definitely could tell the mycelia
was reaching and looked more whispy than rhizomorphic and strandy..

i agree on your lighting. i get away with initially setting the pinset and then sticking the bins in the dark. only giving light a little bit every few days. once they pin...thats all the light they need when its talking about cubensis.

[limeade]

couldn't wait any longer to flip light on and check

these all are way larger.

and abundant, the clusters clusters have clusters.

[limeade]

took a break from work, think we're going to finish building at night the rest of the summer, it's about 106*F without even factoring in the UV index of 9 and the heat index at 114*F, not one bit of cloud cover, 5*rH, and 22mph of "blow dryer" hot wind from the south." 25 days straight of this so far!


but i added some pics.

[limeade]

added some more pics :P

like, still pinning.

[limeade]

is it possible this bag wont quit making pins? and just do this forever? or until it goes dead.

[eatyualive]

palenque is one of those strains that will shoot out tons of pins but very small. very consistently as well. it may just do this till it dies. if you start seeing many aborts. you may need to harvest that. then dunk and the smaller primordia may mature.

[livewire420]

man cant wait to see the final results

[limeade]

Well, these aren't final results, but what im dealing with now.

pretty sad, worst strain ever in my book, solely due to fruit size. (for invitro)
im guessing this isn't a good full invitro bag strain, from all the posts here, i've seen nothing but tiny palenque fruits.

The upside is is BOY do they bruise few pics i bruised a few.

[limeade]

So far I have harvested and dryed 3 oz's. I have a dehydrator FULL now, and 5 more bags to harvest...

My guess is, I've got a little over a half pound dry. When all are harvested.

here is a pic of the blued dry shrooms wet, and dry in the bag, they held the blue

While a half lb of dry shrooms is nothing to be depressed about, i would have expected more from other strains, i am betting this strain would preform VERY well in a "Martha" or in monotubs.

The bioassay says: THIS SHITS POTENT!! I think they're more potent then any PE i've ever eaten, but then again, im just doing my first personal PE grow.

I kept having aztec pattern type (imagine aztec art that swims around!) visuals though... weird,

[Hippie3]

you know,
you always get better results, esp. invitro,
is first you get a good isolate of whatever strain,
going the multispore route is a crapshoot
and the odds aren't real good.

[limeade]

Originally Posted by Hippie3

you know,
you always get better results, esp. invitro,
is first you get a good isolate of whatever strain,
going the multispore route is a crapshoot
and the odds aren't real good.

this is what i hope to be doing with either PE or APE1.0.

im not complaining though! got tons of prints, i'll be video taping my "destruction" of the dry fruits.

I never keep a half lb of dry fruits that's ridiculous.

SOOO, i'll be bbq'ing tonight!

I have long lost my favorite preforming strain, and probably will never be able to recover it, i miss it, it did best invitro for me. Florida wild collection, it LOVED invitro, i think it craved co2.

[limeade]

Total dry now 4.5 oz, another dehydrator full, first flush still. They blue very nicely. aND i realize now it was only a week ago they started knotting, and now i have maybe 6oz dry. They may be small, but the # makes up for it, and they are potent, i could NOT have expected more from bags of straight coir, now i have to say im very satisfied and will do them again, but on manure from a couple of vendors.

[limeade]

Originally Posted by limeade

Well, these aren't final results, but what im dealing with now.

pretty sad, worst strain ever in my book, solely due to fruit size. (for invitro)
im guessing this isn't a good full invitro bag strain, from all the posts here, i've seen nothing but tiny palenque fruits.

The upside is is BOY do they bruise few pics i bruised a few.

lolz, impatience makes u look like an ass sometimes yeah?

retract this statement i've quoted of myself

[lost_onabbey_rd]

lol.. mex pal are one of my favorites.. they reminde me alot of tex, if you can get a good isolate of them going they will make that last bag look like a joke, our old buddy pj used to love the mex pal, actually i think that's were i got my first print of them, one of the few strains i can almost always tell just by looks b/c of the white "socks" they get around the base, every grow of them i've ever seen always has that white fluff at the bottom, which if you care to peals right off and makes for very nice clean looking mushies

[limeade]

i didn't tear it off because it blued so deep... the coir comes off easily after dried, makes good kindling though, BBQ TIME SOON!

[limeade]

I have harvested a total of 6.5 oz's dry, and have 2 bags that need harvested later.
So i think i'll get close to the half pound dry mark off these, more then i expected!

[livewire420]

nice

[limeade]

Originally Posted by livewire420

nice

thanx, about to update my PENIS LOG

[limeade]

I have harvested a total of 8 oz dry on the nose!
AHAHa!

wow, a half pound of dry shrooms, i don't think i can bring my self to burn these up on the grille, they are WAY TOO POTENT...

I have never, ever, NEVER in my life have a cubensis BRUISE WHEN YOU BREATHE ON THEM!!!

as soon as i pulled the blocks out of the bags to harvest, the MYCELIUM bed turned blue, so blue, so pretty.

I bioassayd 1.5g dry last night, had a level 4 experience. These are too good to let go of, the potency is beyond PE... I find it close to a copelandia.

I suggest Palenque for a headstash FOR SURE!

[limeade]

This grow is coming into it's second flush, lots of larger pins yay!

first flush dry harvest off of 14 straight coir bags, 8.25oz dry.
2nd flush isn't looking that great, maybe 5 oz dry i guess so far.

I just mixed up a bunch of wormcastings/coir/verm etc, to start another grow of palenque at my other location. Ill grab a few pics while i'm there tonight.

[limeade]

here is 4 bags

[livewire420]

any prints available?

[limeade]

sure!


check out this mexican fire toe that was in the parking lot the other night:

[livewire420]

that thing is huge

[limeade]

my wife got pissed and dropped our sack of taco's and ran... screamed real loud, it was funny.

[limeade]

just wanted to update, all 14 bags flushed very heavily, more so in fact then 1st flush, total so far has been 1.75lbs dry from 14 coir bags, with just coir/verm/malt/dex/calcium carb, I cannot wait to see the yield from my palenque on compost, soon to come, palenque on 50/50 straw/hpoo, and palenque monotubs. I will probably do one giant tub, and 5 smaller 20gallon tubs.

anyhow here is some recent pics: (check out how blue the base of the stipes are! this is without any physical damage, it did this when it came out of the bag and air got to it.)

[livewire420]

1.75lbs dry from 14 coir bags

[BuckarooBanzai]

That is a nice cluster, man.

Perfect harvest time, IMO. Another 24 hours and they'd have been blowing spores...

[limeade]

Originally Posted by livewire420

1.75lbs dry from 14 coir bags

I was totally shocked too man, I am not really counting on a 3rd flush, but if it does, wow! Thanks for following along with me man. I've enjoyed yours as well!

Originally Posted by BuckarooBanzai

That is a nice cluster, man.

Perfect harvest time, IMO. Another 24 hours and they'd have been blowing spores...

Thanks BuckarooBanzai, I was letting them open up to size, and was quite suprised they weren't sporing, I should have taken a pic of the gills, most specimens were sterile. I noticed this throughout all the bags, there were several that did sporulate don't get me wrong, but sterile gills were abundant also. Which in a way is a plus, opening larger without spooging down the place!


I appreciate you following along as well! This one has been a success!

[limeade]

WELLL I didn't discard all of this spent substrate yet, I dump mine outdoors at a buddy's house that has a house, and a yard, that's fenced in with privacy fence, tree covered, and stays mostly humid and 20*F cooler then ambient temp, So, I am kind of glad, out of 14 bags, this is the 4th or 5th flush, 4 popped these up, the other 10 aren't far behind!
I was shocked!
they look great and still bruising near indigo!

[limeade]

Originally Posted by limeade

This move doesn't affect this thread, as this grow is at my other property,
but the other grows are affected.

I moved the penis envy into the foyer for the 2nd bathroom, on it's own shelf, the closet is now empty that we do the spawn run in, we are going to spawn 15+ more invitro hpoo/wheatstraw bags tonight and fill the closet back up.
We moved the invitro hpoo/wheatstraw onto the dining table in the labroom, and we moved the palenque on hmCompost over to the cloudy blue girly shelf.
So just simply re arranged to make room for more spawn run on the poop/straw, and allow more to come out of the closet.

here are the pics, and i think we'll be making more hash in a day or two off some headies (midgrade) if dude comes through for us.









This update will be repeated per each invitro grow log.

in a nutshell, moved pal poop onto table, pal compost onto shelf, and penis into foyer.

[livewire420]

man your a master at these bags

[limeade]

Originally Posted by livewire420

man your a master at these bags

hahah i try, my wife isn't bad either!

starting ANOTHER new bagthread!