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| Growing Invitro Mushrooms - Classic, Chronic, Bags Fruiting inside jars/bags/etc. |
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| | #1 (permalink) |
| Former Member Join Date: Aug 2007
Posts: 2,272
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ok so this is the thing. i see some stuff here on invitro but not much really. the only reason i think i really like the idea of this is:
i think it would be good for bag growers to post their recipe that they've tried or even how they prefer to do there bags. i know we got crazy1 and limeade that likes bags maybe they can add info to this thread that will help new bag growers such as my self with how to go about doing coir/verm bags, coir/verm/casting, hpoo/straw, hpoo/verm, hpoo/casting/straw/verm/coir/etc. im sure you guys get the point. im gonna be regularly posting in here giving my experinces with the subjust cause as we all know i just failed pe. so im looking to improve what might be the problem. all info welcome, and thank for the one who do give input on this matter. |
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| | #2 (permalink) |
| HippieHugger Join Date: Jul 2008
Posts: 79
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I just nocc'ed up some BRF half pints with all intentions of having them go invitro. I've seen some pretty neat invitro bags floating around, and have been watching the sand experiment closely. Consider sand + supercake + BRF with your invitro mix, and just leaving the spawnbags alone.
__________________ Be who you are and say what you feel because those who mind don't matter and those who matter don't mind. Last edited by inkawgnyto; 08-18-08 at 22:45. Reason: more infoz |
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| | #3 (permalink) |
| Voyager Join Date: Jun 2008
Posts: 1,455
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I knocked up some of Hippie's bags and am currently awiting fruits.One bag was nocc'ed up with 6 cc of b+ and was 100 % around the 7th to 8th day.It's day 10 and am seeing lots of knots .I duct tape my bags to coat hangers and then throw a shirt over them like a normal shirt in the closet.This helps disguise it and helps block light
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| | #5 (permalink) | |
| Former Member Join Date: Jul 2008
Posts: 1,182
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | Quote:
i haven't had a monotub grow in some time because of the space, time, care, and attention they require, bags i can focus on more important things. i would consider bags a huge success because they are little microclimates, they totally take care of theirselves, tubs u have to mist/fan etc.. i started noticing monotubs benefited more from care then neglecting it, they work with neglect, but they do better with care, bags just work with neglect, getting a proper filter patch bags is vital. there are different types 3a is more for colonizing 3b is multipurpose 3c is fruiting only. 3b unicorn filterpatch bags are top notch. coir substrates do work, but aren't up to par with using manure/straw based substrates or compost. I haven't tried any of hippies mixes, maybe i will when i can find some time, I am pretty lazy i order my substrates. but that's the beauty of it, it works for all of us. I am sure I can try to do my best to answer any questions, oh, and use paperclips to close up your bags, after rolling it over a few times, tape gets tiresome, impulse sealing becomes bothersome, and unnecessary, lable and date your bags, mark the flushes. k, off to update my penis envy log. It's invitro bag with compost. | |
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| | #6 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
![]() ![]() ![]() | Well
I'll get an invitro grow going today for ya. I'll be using compost for this grow. As I have a lot that I made. hehe Now the strain will be PESH that I have in an lc right now. Here I don't really hang em up in a closet or anything like that, they'll just sit on a shelf and grow from there. It will be done in a filter patch bag noc'd up with the lc. pics to be added tomorrow am. Great thread Live
__________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience |
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| | #7 (permalink) | |
| Former Member Join Date: Aug 2007
Posts: 2,272
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so how can you tell the difference in what your working with? Quote:
Large Presealable Autoclavable Gusseted Bulk Spawn Bags w/ Self-Healing Injector Site #16250 Set of ten bags per offer (select larger sets below for bulk discount). Features microporous filter patch for contaminant free gaseous air exchange and self-healing injector site for syringe inoculation without taping! Ideal for spawning grain, sawdust, or straw. In many cases the bag can be used as the fruiting container. New model filter design allows bags to be presealed before sterilization. Measuring 18" high * 8" wide * 5" deep. Large Presealable Autoclavable Gusseted Bulk Spawn Bags #16248 Set of 25 bags per offer (select larger sets below for bulk discount). Microporous filter patch allows contaminant free gaseous air exchange. Ideal for spawning grain, sawdust, or straw. In many cases the bag can be used as a fruiting container. New model filter design allows bags to be presealed before sterilization. Measures: 18" high * 8" wide * 5" deep. because none of these say anything about what type of filters? infact none of the sponsors here or at a few other place offer nothing but spawn bags. | |
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| | #12 (permalink) |
| Former Member Join Date: Jul 2008
Posts: 1,182
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | nice! i've always assumed the ones with injector ports were for colonizing only. But i make sure mine are 3b, if you "search forum" for invitro, you'll see numerous threads by me, "Invitro (strain here) on (substrate here). |
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| | #15 (permalink) |
| Former Member Join Date: Jul 2008
Posts: 1,182
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Live, if you need some more PE spore i can get it to you bro. I have a few coming up that look RATHER printable, very dark underneath. I'd like to see your penis in a bag, I updated my invitro penis on compost, i think you'll like the genetic stock.
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| | #16 (permalink) | |
| Mycotopiate Join Date: Jul 2008
Posts: 405
![]() ![]() ![]() ![]() ![]() ![]() ![]() | Quote:
Holy crap thats one hell of a super cake formula. My FOAF nocc'ed his cakes on the 12 and now that I looked at them better I would say their only 8% done(just BRF ,verm and 1/3 cup of water per 1/2 pint jar). I am starting to think maybe he should look into that formula. I guess thats a prime example why its called a super cake... | |
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| | #17 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
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hahaha that's why it's called a supercake! I'll post the pics of the 4 bags I have going here in an hour or two. Gotta find the freakin camera lol
__________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience |
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| | #19 (permalink) |
| Former Member Join Date: Aug 2007
Posts: 2,272
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yeah id love to get a print. so when adding sent coffee how much do i add? ya i never got to finish then bags out ended up moving cause of other things going on, but my 30 tc clone jars been ready to roll. plan is casting/coir/verm 2c of carb i want to try out the coffee this time. if anyone knows the correct dosage i need to do. lime- mail coming your way |
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| | #20 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
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well here are 2 of the 4 compost invitro bags of compost spawned with 60ml of pesh LC. ![]() no colonization yet, but I'll keep this updated
__________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience |
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| | #23 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
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Didn't have spawn, just an LC, so I figured wtf and went with it. I do most of my cakes and bags out of LC. Have good luck with em too. hey do you add that coffee when mixing for the soak before pasteurizing
__________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience |
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| | #24 (permalink) | |
| Former Member Join Date: Jul 2008
Posts: 1,182
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | Quote:
You can use cakes as spawn bro, makes great spawn! I wouldn't do supercakes for spawn as those seem to be used for straight fruiting and your bulk sub will have all that it needs! | |
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| | #25 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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Cakes are fantastically useful in this hobby, even if you never fruit them. They make excellent spawn - a stand up vegetable grater will reduce a cake to a million points of inoculation in a very short time. Cakes are also a very cheap and easy way to test an LC or questionable syringe.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #26 (permalink) |
| Former Member Join Date: Jul 2008
Posts: 1,182
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cakes make great gifts for like minded friends, especially done ornately in a fishbowl on a bed of chosen marbles, i like choosing a bowl with a lid. i handed out a few for christmas last year, had it timed to where by new years 1st flush was coming up right on time for fresh doses on new years. The old hippies i gave one two thought it was just the best gift ever, especially since it kept giving, he used the prints from them to start LC's and then grains to do a lot of outdoor work on his farm.
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| | #27 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
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yup Buck cakes do make great spawn. This is the mega cake I shredded up like you said to a bulk sub. ![]() The cheese grater works like a charm
__________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience |
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| | #28 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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Gah - I know what you mean. I'm jacking his thread talking about cakes and have yet to mention my own fondness for spawn bags! Hell, I even wrote up a Tek about it. Back on topic, damnit! Total apologies Livewire! BB-Nug Tek - popcorn/coir/castings in spawn bags: http://forums.mycotopia.net/popcorn-...llon-jars.html (Buckaroo Bulk Nugget Tek (invitro, spawn bags and/or ½ gallon jars)) Invitro BB-Nug with her bag pulled off: ![]() And man-o-man do I love those presealable spawn bags. So much less hassle. I don't use the injector port types, though. A blob of silicone with some duct tape over it makes a great inoculation port. Don't forget the duct tape, 'cause the silicone blob will sometimes pop right off the spawn bag without some support. And don't put the duct tape on before the PC run. Duct tape melts...makes a sticky mess.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #30 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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Dig it. Castings and coir. I like to add poly, others like verm, some just use the castings/coir. I think the poly makes the best water reservoir - and I hate screwing with the dunk...
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #31 (permalink) |
| Former Member Join Date: Jul 2008
Posts: 1,182
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Hah that's a most excellent tek!! I just read through it! this is what this thread needed, i simply follow hippie3's repost of hydr0420's method, i like the grains colonizing within the bag though, that's interesting! it eliminates a process for sure! while we all got off topic a little with cakes, lets re assure anyone that is not wanting to step into grains, but want to go bulkish, that you can continue doing cakes, and use a food grater to grate it up, and use your cakes as spawn! There isn't a need to do any special cake formula either, since it's just being used as spawn, the most basic flour/verm cake can be used. So cakes do have a little bit o place in this thread, just as spawn though! and bonzai im just guessing because all things are possible, but have you tried a grain to grain type transfer into those bags? i bet that'd shave a day or two off the time! Last edited by Beastmaster; 08-23-08 at 16:36. Reason: Triple post, each just a couple minutes apart, give me a break |
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| | #32 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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No attempt at a G2G. I don't like to open the bag until I'm doing the first harvest. I inoculate the popcorn layer with about 90-120mL of hot/fresh growing LC. No need to worry about over wetting, all the coir/castings under the corn soak it up. I always move the needle around a lot, soaking the top layer of corn and getting plenty to drip down the sides of the bag. 60mL syringes are our friends. The popcorn runs FAST. Usually 4 days at 86F. After mixing, the nugget is usually done in another 5-6 days (depending on volume). I start giving 'em light after day 3 if they are past 80% colonized. And it takes 'em a little longer, but I don't do any squeezing for fresh air exchange. I love those friggin' filter patches. They are a thing of beauty.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #34 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
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OK here's a grow I"m doing invitro. The other bags don't seem to moving too fast. So the other day when I was breaking up the supercake with sand in it I figured I'd start another invitro bag. This was mixed at about 50/50 spawn to pasteurized compost with 20% or so verm in it. This was inoculated 5 days ago. I know it ain't the best pic but you can see it lol
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| | #36 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
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Thanx Live, I'll keep it updated as things progress. And since this is being done invitro, it has been given light from day 1. As I know some have been asking this in other threads.
__________________ Are you a human being having a spiritual experience, or a spiritual being having a human experience Last edited by crazy1; 09-01-08 at 07:43. Reason: added last part |
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| | #38 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
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Hey Live, I really don't think that not exposing it to light from day 1 would have any adverse effects on the grow. It just speeds up the time to pin.
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| | #39 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
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OK we have LIFT OFF!!!! hahahaha wOOt ! ![]() Here is a pic of the one made with the supercake with sand in the mix. ![]() I know the pic aint the best but enjoy.
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| | #41 (permalink) |
| VIP Member Join Date: Apr 2007
Posts: 874
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thanx lime, I didn't have to adjust the ph at all really. The compost it was spawned to was neutral as it was aged well. Man I've NEVER had luck with coffee at all man. Goes green on me every time i there's coffee in it.
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| | #42 (permalink) |
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| inverto is the sh*t
I use popcorn and grow invetro. I take a pressure cooker fill it up 1/3 of the way with popcorn and then the rest of the way with water an inch or two from the top of the pressure cooker. I then pressure cook it at 15 psi for 30 minutes it takes a while to get going because of all the water and popcorn. I then take it and strain it and rinse if off real good because of the starches. I then put it out on a tarp and put fans on it until it feels pretty dry to the touch . I then take my jars put a 1/4" to 1' of verm on the bottom then fill the rest of the way way with popcorn about 2/3 full so you can shake it later if you wanted and pressure cook at 15 psi for an hour. The reason I put verm on the bottom is to soak up excess moisture. This has never failed me lots of years doing this the only problem I ever have is once and a while I get wet spot bacteria only because the popcorn was a little to wet. 90-95 success rate though lately. I then pasturize straw, cow poo, and verm. I use the filter patch bags. I do some staight straw or poo and verm, or all three together. I take popcorn and straw,verm or whatever and layer it a couple of inches and push all down hard and seal the bag when colonized cut bag open put polyfill in the top part of bag and put a fan on them 24-7. I used to do tubs but if the tub goes bad the whole thing gets tossed here if one bag goes bad it's less loss then a tub . I think it's more efficient going invetro. For the area you are using you get more bang for your buck. |
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