Hip's invitro tek [merged]

Would it allow for better growth if som of the Vermiculite layer were let off to make more room for the fruits to fill?


Needs of the many outweigh the needs of the few

It's common for invitro growers to open the jar up after full colonization to dump the verm barrier out. Don't leave it out from the start as that's your filter to protect from contaminants.

there really is no need to make more room,
invitro will still form fruits
in a full jar.
i just shake off the loose verm after first harvest

[cutty]

there really is no need to make more room,
invitro will still form fruits
in a full jar.
i just shake off the loose verm after first harvest

Its amazing how the fruits just force thier way against the glass and make room for themselves.

[Lazlo]

The cake will shrink a little bit because it's loosing moisture.

I want to do a bulk invitro grow. Ive read something about popcorn invitro and amazing results, can anyone give me a link to a popcorn invitro tek? I haven't found one that explains it yet.. if there is one. But anyways, what tek is the way to go for best yeilds from invitro?

[Lazlo]

I would think BRF in 1/2 gallon widemouths for bulk invitro. Damn, i just thought of that! Sounds pretty cool huh! Damn, i might have to give that a shot. I'm just throwin you an idea.

(Message edited by looper on February 16, 2005)

well ususally grains do fairly poorly invitro from what i've heard.. i'd be interested in this too if anything turns up...
LOST

[cutty]

I do many invitro grows,and I find that the BRF tek works the best IMO,I have tried grains and popcorn and I find the yields arent as Plentiful.

[Lazlo]

Whole grains alone don't hold water like the BRF. I just ordered some 1/2 gallon tapered wide mouths and i'm going to give it a shot. With ground up bird seed though.

(Message edited by looper on February 16, 2005)

[cutty]

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

I would think BRF in 1/2 gallon widemouths for bulk invitro. Damn, i just thought of that! Sounds pretty cool huh! Damn, i might have to give that a shot. I'm just throwin you an idea. <!-/quote-!><hr size=0></blockquote>A foaf has 2 (1.5 Litre) Mason jars half way colo'd right now with BRF + Verm.I'll get him to let one go invitro and post pics.These jars do take a while to colo though.It will probably take atleast another month to finish colo.

[Lazlo]

I would think with karo i can get them colonized in 2 weeks. If the formula works with the WBS. I don't see why it won't. How long did your buddy pc the jars?

[cutty]

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

How long did your buddy pc the jars?<!-/quote-!><hr size=0></blockquote>90 minutes...He nocc'd em up with multispore 10cc in each jar.I know 10cc is alot but hes tryin to get the jars to colo a lil faster.Its already been a month and there about half colo now.

[Lazlo]

Yeah, with the karo i should be able to coat most of the cake with it, speeding up the colonization considerably. Awesome! Thanks Cutty.

[cutty]

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

Yeah, with the karo i should be able to coat most of the cake with it, speeding up the colonization considerably. Awesome! Thanks Cutty.<!-/quote-!><hr size=0></blockquote>Np bro, Yeah I would agree with ya, Karo or any liquid culture would definetely speed up colo.

I've had some success with rye berries and invitro growing. If you use quart jars of rye, they'll fully colonize in two weeks, and within a month to six weeks you'll have good results

[pix lost so deleted]

[Lazlo]

Very nice. What strain is that? And how do you get your fruit out of there?

Just reach in and dig em out with a fork.
PR's on top and BR on the bottom.

Curry had some nice results with a substrate of 50/50 millet/verm.

Check out the tek here:
http://mycotopia.net/discus/messages/5/175850.html ?1098884276

If you want results like Rodgers jars above,
strain isolation on agar is the way to go.
Isolate a strain that easily fruits on agar and it should do great invitro.

[Lazlo]

I'll skip the agar. For clones i like karo. I do want to try out Stephen Peele's hot agar tek cleaning up prints.

[cutty]

Wow Awesome pics RR,Ive never had much success with Rye with Invitro but then again RR you always amaze us here at Topia.

[candykid420]

You should do what I'm going to do for my bulk Invitro grow:

200 full-pint jars

i sure wouldn't go popcorn invitro
at least not without
first isolating a proven substrain.

[Lazlo]

200 jars! That's all i need is more jars. I bought 6 of these 1/2 gallon bastards last night and don't have the room for them. My girlfriend surely wasn't happy either. I had been drinkin and felt like experimenting!

Regarding Invitro-

I think I remember seeing a pic posted awhile back of a light fixed to the underside of a rubbermaid lid to initiate pinning/fruiting and provide an automated setup since the light was on a timer. I've searched High and Low but have been unable to find that pic... Does anyone use this setup or know of a cheap light supplier. I'm already familiar with tap lights, I'm interested in automation. Thanks!

[Lazlo]

Check out Insight's rig in the thread here in fungi called, Lighting My Fruiting Chambers.

thread here in fungi called, Lighting My Fruiting Chambers.
that would be http://mycotopia.net/discus/messages/2/190742.html ?1108446638

that thread was exactly what i was looking for... awesome guys.

200 full-pint jars
holy shit-you the man!!
...I guess you just got a lot of really hungry friends hhuh?

[morthos]

Nice numbers Candykid420. Does the brf mix do as well in 500ml jars as it does for 250ml? Are there any drawbacks to that size? I would think you'd fruit them upside-down like Chronic tek, with a few holes in the lid for air exchange?

I think the fastest way would be to spawn with existing brf cakes...I'm thinking if you sterilize a bunch of 500ml jars of brf mix, you could spawn from 250ml jars, ending up with a bunch of 500ml invitros.

What's your method with 200 500ml jars Candykid420? How long have you had that setup running?

Are there any drawbacks to that size?

colonization time.
if you really wanted true bulk invitro
in a reasonable time-frame
my approach would be
brf/verm mix in mycobags
so you can knead/squeeze them to speed up colonization
then once fully colonized
one could cut away bag
and pack colonized substrate into
whatever rigid container one chooses,
even half gallon jars.

Wow! those look awesome RR, so you just used rye berries and some coffee grindings and that's what you got?! What was the ratio of coffee grindings to rye berries? Very nice, I love the bottom picture!

[Lazlo]

It looks like he's got a good clone going on those puppies!

[candykid420]

Morthos, there is no secret to the setup I have, just lots and lots and lots of rubbermaid bins, filled to the brim with jars. I use seperate lamps overlooking the tops of the bins to give them light. Nothing to it

And the mushrooms fruit just as well in 500ml jars than in 250ml, if anything you get more out of the 500ml because they have room to grow longer and fatter. I get at LEAST 3 grams dry off of one 500ml (as opposed to 1-2 grams with 250ml), plus it will flush again.

It just takes a LONG time to make and cook 200 jars (but the process becomes much shorter with the help of four pressure cookers).

I know there is a way for better yields (straw logs, casings) but if you want bulk INIVTRO, then my method is the best way. There is only so much one can harvest from a single invitro jar, you might as well make a ton of them if you want bulk.

[morthos]

(So you are making cakes twice the normal size, right?)

Nice. I once was up around 40 250ml jars, regular PF cakes; some of them I fruited inside 500ml's, but making the cake that size in the first place makes more sense. I had a smaller PC back then, mind you...wasn't possible.

I used standing metal racks with white sheets draped over them, using ambient light from windows. Of course, I dunk between flushes and bleach dip starting after the second flush.

Four pressure cookers! I love it! I'm tempted to ask what your weekly dry harvest is, but that might be the wrong Q to ask here.

How are you inoculating your 500ml brf jars? I would think the quickest method for colonization would be spawning...what works most quickly for you? Is using spores on large jars very slow? If spores, are you using only 4 inoc points, or more?

May I copy your brain? I am playing with popcorn res effect and soon wbs too but invitro is the most reliable method I've done. Plus I don't want to be scheduled into misting, etc. I would like to see what potential your method yields.

I'm curious as to the subtleties of your operation; 1. Do you label the lids of jars to have an idea later when one should be thrown out - you can't keep tabs on if a jar has fruited in the last 2 wks.

Thx!

[candykid420]

wrote you an email

[morthos]

email replied to, thx!

Thanks for the reply Hippie3, I'll read up on mycobags.

I suppose a working method I already have would be to use colonized 500ml brf jars as spawn with sterilized 500ml jars of substrate, load into 1.5L jars inside a glovebox...probably much more work though!

At the moment I'm trying invitro with res-effected popcorn. I've got a 2-jar popcorn & verm cake upsidedown in a transparent tupperware container with holes in the lid. I also have four coir-popcorn res cakes with some verm on top and underneath in rectangular tupperware w holes in the lids. That's two 500ml jars of popcorn mixed with verm or coir in a 1:1 ratio, with about an inch of airspace above them in the containers, and a quarter-inch on the sides. Think they'll work?

Neglect teks feel time effiecient to me...

RR, Very nice invitro Pics!

I know this isn't orthodox, but it worked somewhat. I made some jars from castings, coir, and verm, although I didn't exactly measure it, and I used dry layers top and bottom pf style. I do know that castings were the largest slice of the pie here. Well, I shot em up with pan spores, and after reading Roger's comment about nobody shoots up poo with spores and waiting like two weeks with no growth I took em out thinking I wasted my time. Then there was growth. So I put em back and waited another week and a half. My original idea was to get a cake that would fruit in a terrarium, like an easy pan tek, so I birthed one I put in pinned and I actually got a few tiny pans. Not worth trying again - for one the mycelia was sparse and doesn't really hold the cake together like with brf. So I decided to use the rest as spawn. I spawned some of them to pasteurized straw mixed with buffalo poo and man that colonized fast. My other thread shows the casing I fucked up. Well, I saved a few to do another casing, but one had an invitro fruit growing, and knowing how poorly they performed in a terrarium and not wanting to use that one for spawn, I let it go to see what I would get. I know these aren't regular size pans or anything, but the jar has already produced more invitro than the one I birthed. This jar might end up producing enough for one person to enjoy, but I doubt it. Anyway, there are like twenty something little pan pins in there. Probably not worth the effort, but with some tweeking could this be a way for someone to grow pans stealthily? So I guess there still hasn't been produced a worthwhile pancake, not that I have seen anyway, but I did get more than what was shown on the one in the archives.

oops forgot the pics

Very cool. Dig them out and clone one, eat the rest.

[Hippie3]

so cute.
i'd say you may be on the right track,
just need a better formulation,
maybe a bit less poo ?

Maybe. I will try again sooner or later, but this is def not giving me enough weight to recommend to anyone else yet. The casings I spawned this mix to colonized fast but I must have fucked up somewhere, proly casing and moisture upkeep, because I have yet to get a single pin on any and I have three in the chamber. I know my cool mist got clogged and casings dried, hell I haven't even had luck with my normal casings in a long time. The first couple of casings I did were straight grain and I had luck, but that must have been beginner's luck. Maybe I should take a trip to see the shroom gods, it's been awhile, maybe they are pissed. Anyway here are some pics.
the first is the same cake different jar, the second I stirred the casing and misted because it got dry, the third seemed moist still so I let it be, and the fourth is the one in the thread pan casing that I have removed most of the casing from and intend to recase.

[nomoreusmc]

I think you need to make a little mare space in there for them, like goldfish won't outgrow there pond

[shitep0ke]

This is my first post but I've been doing a lot of reading in the archives and in the forums and have read quite a few teks and have finally decided to give this a shot. What really interested me in the archives was the Hominy project. I read through the whole thread and really liked the idea of a cheap and easy to prepare substrate. My main question is can you use Hominy to successfully grow invitro? I know grains are generally used for casing and some mentioned varied success using Hominy to spawn. In the Hominy Project thread it seemed like the majority of the interest was to find an easy to prepare and use substrate for new growers, if this could be combined with the apparent ease of invitro growing it seems as tho it would be an ideal setup.... not necessarily for the best flushes, nicest fruits or even fastest colonizations but mainly as a really easy and nearly fool proof way to grow. I have also been gathering info about the varied success of sterilization using the microwave. I know it is shaky at best but it seemed that a lot of the failures were due to an excessive drying of the substrate. If this were the case, and Hominy could be used to grow invitro, it would also fit that Hominy would be the ideal substrate for anyone without a PC/stove since it might hold up better in the microwave due to it having a higher water content than other substrates.

I know that 99% of beginners fail because they deviate from established teks, but in my case I don't think any conventional tek will fit my situation (mainly the lack of PC/stove). My plan is to grow invitro (verm layer on top and bottom) on hominy using the microwave to steralize and I want to use the brazilian strain for its fast colonizing which will hopefully aid in fighting off contams (will Brazilian grow invitro? If not will Z's?).
My whole plan basically hinges on the idea of using hominy as an invitro substrate so if thats not possible I guess this a moot post for the most part. However if it is possible, what do you all think of using hominy as a micro-steralization substrate? I think I have read through most of the archives about micro-teks but that is the main area where I am lacking a lot of info, do any of you do it? Have you? Is it naive of me to think I can pull it off?

thanks
-s

[pskovinsky]

Z's are all about invitro, but most any cubie strain works well for it.

Hominy on the other hand, does not, i got one jar to fruit invitro through abuse, but in general they won't do it.

A casing is VERY easy though, heres what i did:

Get a WIDE jar, like 6 inches wide kind of wide, and at least 6 tall as well, check the doller store.

Crumble (gently!) the grain (hominy, in this case) and try to get it as level as possible on the bottem of the jar, then take your damp verm (dampen it till you can give it a good squeeze and only get a little drip or two of water out them bottem), and spread maybe 1/4-3/8ths of an inch of that over the top of the grain.

Layer some duct tape around the bottem of the jar so that the grain isn't exposed to light.

Now put the lid back on the jar, and put it in a warmish spot with light (65-75 degrees. 70-75 is best), at LEAST twice a day (preferibly three times) take the lid off and use it to fan the old air out of the jar, 10 seconds of fanning is enough.

In a week or two(or three, or four, sometimes), it should start popping little pins up, if it doesn't keep this up.
Should the verm start to dry out a bit, lightly mist it with water, LIGHTLY! Don't mist if you can see little white round thingies (hyphal knots) or tiny tiny mushrooms (pins), as it can kill them.
Because the lid stays on, you don't lose nearly as much water to evaporation as you do with a normal open casing, so you probably won't have to mist till the first flush is getting largish, but your mileage may vary.


Thats what i did my first grow, which happened to be with hominy. I didn't get a nice even flush like the pics you'll see, but i got a bunch of mushrooms from it.

[Hippie3]

i think one could get hominy to do ok invitro if one went with the rez-effect concept.
i'd intermix the verm [coir even better] 50/50 with hominy
then put the filter/barrier of verm above like a cake.
then steam it an hour, maybe even two.
'wave sterilization is tricky,
you might get a few thru ok
but also expect to lose several.
steam is better.

[bipolar]

If you don't have a stove, get youself a single or double burner electric hot plate. That with a big enough pot will give you a good steam source. I've been steaming all my BRF 1/2 pints up til now. I've finally got a pc.

Peace

[inexorable]

Hey! Really need to invest in a fuckin digi-cam. Anyhow, just wanted to say that I noticed shrooms growing on a fuly colonized 'cake' of birdseed. I was going to use this birdseed-only jar as grain spawn, but it got misplaced till now, and the shrooms are pretty big inside the jar!! Since it's fully colonized, I suppose I could pick them, and keep growing invitro (but because grain contaminates soo easily I'll just pick em and use it for spawn). No huge discovery--just didn't know if anyone had invitro grain cakes before. Hippie3 Invitro Tek = adaptable.

[Hippie3]

it's a bit rare but not unheard of.