an observation about trich

~Hippie3 · 11-11-07, 07:28

i've noticed that, on close examination,
almost all my incidents of trich involve
a hair that has fallen on the casing surface.
so i did an experiment-
i placed some wet coir in a bucket
and placed a few hairs on it.
sure enough, the normally very contam-resistant coir soon developed a patch of trich near one hair
and some cobweb mold near another.
so might i suggest that
folks should wear hair nets or shower caps, etc.
when working with their projects
and keep an eye out for any loose hairs.

mrpanda · 11-11-07, 07:32

mmmm maybe thats why my contams are low. I don't have much hair and what I do have is locked in gel most of the time.

camMyco · 11-11-07, 07:32

Thanks, Hippie! I've always followed the belief it was from skin cells (learned from you) so hair would be a great source of Trichoderma.

~Hippie3 · 11-11-07, 08:34

whiskers, eye-lashes, arm hair, nose, etc.
all bad.
cover as much skin/body area as you can
[clean clothing, gloves, head cover, face mask, etc.].

syvel · 11-11-07, 09:17

Interesting.

spacecake · 11-11-07, 10:22

A shower before working with your mushies helps alot too !

LotRev · 11-11-07, 10:46

That is not good news, as my dog sheds to no end.

My own hair is hard enough to keep up with.

Every project done around this area with this dog around
has gone the way of trich.

~Hippie3 · 11-11-07, 10:53

cats too

bugs · 11-11-07, 10:58

Makes sense. Hair's hanging out in the breeze, and catches all the stuff flying around.

Foster · 11-11-07, 11:06

Good to know hip

. Maybe i will order that tyvek body suit. Foster

camMyco · 11-11-07, 16:55

I guess ...Derma...should be the first clue.

abbr. · 11-11-07, 21:24

This past summer a friend and I
battled with trich, he has cats and dogs
at his house, and at my house,
Im constantly finding my hairs on
my pillows, hats, shirts, hmmm
Must be getting thin up there

But stray hairs could def. have
been the source.
We could not narrow down
exactly where the trich was coming from.
Tried to figure out if it was the
straw, the h-poo, the casing materials,
our pasteurizing!
Racked our brains for days!
Hopefully this will make all the difference.

MrMycelium · 11-11-07, 21:28

My hair is almost down to my ass, I am doomed. lol

greenweanie · 11-12-07, 14:42

thanks for the observation hippie. i should have noticed that right away

my old house - no pets. the gf's house, dog and soon to be a couple of kittens. guess its time i make a larger glovebox for doing larger projects than basic bread tins

twoguysupnorth · 11-12-07, 15:23

thanks for the info. well there is no way around a glove box here i bet. a few pets and a dusty old house. i havnt even tried yet, but i was seriously concerned about the dust already. any tips? because there is no way around it.

NEPHROSIS · 11-12-07, 15:33

Good to know, glad I'm bald hehe.

scientia · 11-12-07, 15:38

I have two cats and I really don't have any problems with contams. The house isn't very clean either, and was probably built in the 1800's. It's all about being clean when you need to.

I use a glove box to do ALL innoculations, agar work, printing, everything. I also don't let my cats into the room where I keep my fruiting chambers, and still ozium it down before spawning anything. Also, a little bit of bleach in everything helps too.

In short, don't get rid of your animals/shave yourself. Those tyvek suits ARE surprisingly affordable though

.

hour · 11-12-07, 15:58

Quote:

Originally Posted by NEPHROSIS

Good to know, glad I'm bald hehe.

Yeah - atleast we have one reason to be happy about balding.

bear · 11-14-07, 06:53

Yeah.. good observation - it seems to agree with the general issues around here.

myco-curious · 11-14-07, 13:40

When inoculating, those are a lot of the same precautions I take. After setting up the environment and while allowing the dense fog of oust to thoroughly settle in the room undisturbed... I do the follow regime.

Turn off the HVAC unit for the house, throw a freshly laundered pair of scrubs into the dryer/tumbler and head off to take a shower. I shave, brush my teeth, use mouth-wash and get dressed with the still warm scrubs. I then pull my hair back into a tight-fitting cap, put on latex gloves as well as a set of new tyvek wrist sleeves. I then put on a surgical** mask (which is not the same as a dust mask) and a pair of safety glasses. I definitively look like a fool at this point...but I'm ready to enter my now (reasonably) sterilized inoculation environment.

---
That being said, once I throw a colonized tray into the fruiting chamber I've been thoroughly neglectful of any form of sterilization procedure - hell the damn thing sits right next to an old deep-freezer that's died on me several months prior, but have only had one case of trichoderma show up so far and that was out of this grow...and I would be damned if the transport into the chamber wasn't something like a hair or whatnot.

** dust masks are designed to protect you from inhaling rather large particles from the environment, surgical masks are designed to stop your exhaled breath from entering the environment and potentially contaminating a patient. They're not interchangeable...

twoguysupnorth · 11-14-07, 17:53

yeah there is one room in the house where pets arent allowed. i dont think i would stick with it if i had to wear surgical masks and stuff(just out of my realm for things i want to do) a little work isnt bad though. ps, and i am only bald by choice about half the time,lol. ill take the extra measures but if it really turns out to be so hard, i'll leave it to the proffesionals and read about it.

OG Royal Grower · 11-14-07, 18:16

whats the best way of printing when trich is present? any precautions different from typical prints

~Hippie3 · 11-14-07, 18:20

Quote:

Originally Posted by OG Royal Grower

whats the best way of printing when trich is present? any precautions different from typical prints

i don't print anywhere near contams,
my lab hasn't had a contam in it in years.
2 different rooms/places,
don't grow in the lab.

OG Royal Grower · 11-14-07, 18:23

what about choosing a strain because of its ability to grow regardless of contamination? Is printing not an option? is cloning possible still?

~Hippie3 · 11-14-07, 18:30

no such thing as a contam-proof strain
many things are 'possible'
yet not advisable.
i would not clone from any contam'd tray either.
the best option when you know your print is likely contam'd
involves transferring to agar, multiple times,
to isolate good clean mycelia for lc to spawn , etc.

vinz · 11-14-07, 20:50

nice one hip

il try hair on agar one day if i have time hehe
or someone else beat me to it..

mikeg · 11-14-07, 20:54

i have a walk in closet committed solely for lab purposes since i have four cats. is this fine? i was thinking of getting an ionizer too. i have only went through a bout of contams this week and its a black mold not trich. but i do see cat hairs in my popcorn jars all the time and they colonize fine. have even seen some hairs on casings but i just gently pick it out

heritage ranch · 11-09-09, 22:44

Quote:

Originally Posted by Hippie3

i've noticed that, on close examination,
almost all my incidents of trich involve
a hair that has fallen on the casing surface.
so i did an experiment-
i placed some wet coir in a bucket
and placed a few hairs on it.
sure enough, the normally very contam-resistant coir soon developed a patch of trich near one hair
and some cobweb mold near another.
so might i suggest that
folks should wear hair nets or shower caps, etc.
when working with their projects
and keep an eye out for any loose hairs.

interesting observation

i recently went to school to become an EMT and in the chapter of our text book that went over medical terms i cam across this

combining form
trich/o

meaning
hair

coincidence? i think not

i dint make the conection till i read this thread, thanks for the heads up

bmad · 11-10-09, 00:34

wow heritage that post is two years old.. no one can say you are not doin your research..

Thanks for bumpin it though this is something I didnt know

heritage ranch · 11-10-09, 02:07

plenty of good stuff to read thru here, gona keep me busy for some time

GadgetGuy · 11-10-09, 02:23

Quote:

Originally Posted by hour

Yeah - atleast we have one reason to be happy about balding.

Bald men get more head

August West · 11-10-09, 02:35

i'm gonna start calling my
tricho cereus', hair candles

and now, back to the topic.

11-10-09, 06:53

hip have you got a chance to put ur uv light to it?

zodd · 11-10-09, 07:11

ill keep this in mind thanks

~Hippie3 · 11-10-09, 09:52

trich ?
no,
so far i'm just using the uv wand to clean up foil prints,
and my flowhood work area.

eatyualive · 11-10-09, 11:17

uh oh looks like a trich war is brewing.

copelandiaKidd · 11-10-09, 11:32

wowza. that is a great observation!!!!!!!! I will run some tests myself!!!!!!

zodd · 11-10-09, 12:20

might be worth testing if pasteurised hair is just as prone.
just a thought

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11-11-07, 07:28	#1 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,765	an observation about trich i've noticed that, on close examination, almost all my incidents of trich involve a hair that has fallen on the casing surface. so i did an experiment- i placed some wet coir in a bucket and placed a few hairs on it. sure enough, the normally very contam-resistant coir soon developed a patch of trich near one hair and some cobweb mold near another. so might i suggest that folks should wear hair nets or shower caps, etc. when working with their projects and keep an eye out for any loose hairs.

11-11-07, 07:32	#2 (permalink)
mrpanda It is big and I am clever Join Date: Jan 1970 Posts: 893	mmmm maybe thats why my contams are low. I don't have much hair and what I do have is locked in gel most of the time. __________________ Weeeeeeeee. That is all.

11-11-07, 07:32	#3 (permalink)
camMyco Modified Honey Join Date: Feb 2007 Posts: 2,141	Thanks, Hippie! I've always followed the belief it was from skin cells (learned from you) so hair would be a great source of Trichoderma. __________________ “ART IS THE ONLY WAY TO RUN AWAY WITHOUT LEAVING HOME.” — TWYLA THARP

11-11-07, 08:34	#4 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,765	whiskers, eye-lashes, arm hair, nose, etc. all bad. cover as much skin/body area as you can [clean clothing, gloves, head cover, face mask, etc.]. Last edited by Hippie3; 11-14-07 at 07:31.

11-11-07, 09:17	#5 (permalink)
syvel Mycophage Join Date: May 2007 Posts: 199	Interesting.

11-11-07, 10:22	#6 (permalink)
spacecake Universal Mod Join Date: May 1972 Posts: 4,810	A shower before working with your mushies helps alot too ! __________________ "As a child, i could walk on the ceiling. I'd butterfly up on the walls"

11-11-07, 10:46	#7 (permalink)
LotRev Technopagan Join Date: Apr 2006 Posts: 1,128	That is not good news, as my dog sheds to no end. My own hair is hard enough to keep up with. Every project done around this area with this dog around has gone the way of trich. __________________ If you want to keep things moving, you should free the elements. Some people call that chaos.

11-11-07, 10:53	#8 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,765	cats too

11-11-07, 10:58	#9 (permalink)
bugs GO LEMMINGS GO! Join Date: Apr 2006 Posts: 1,884	Makes sense. Hair's hanging out in the breeze, and catches all the stuff flying around. __________________ Bagseed is like a box o' chocolates. Ya never know what you're gonna get.

11-11-07, 11:06	#10 (permalink)
Foster intotheoutside Join Date: Jun 2006 Posts: 1,854	Good to know hip. Maybe i will order that tyvek body suit. Foster

11-11-07, 16:55	#11 (permalink)
camMyco Modified Honey Join Date: Feb 2007 Posts: 2,141	I guess ...Derma...should be the first clue. __________________ “ART IS THE ONLY WAY TO RUN AWAY WITHOUT LEAVING HOME.” — TWYLA THARP

11-11-07, 21:24	#12 (permalink)
abbr. Mycotopiate Join Date: Jun 2007 Posts: 268	This past summer a friend and I battled with trich, he has cats and dogs at his house, and at my house, Im constantly finding my hairs on my pillows, hats, shirts, hmmm Must be getting thin up there But stray hairs could def. have been the source. We could not narrow down exactly where the trich was coming from. Tried to figure out if it was the straw, the h-poo, the casing materials, our pasteurizing! Racked our brains for days! Hopefully this will make all the difference. __________________ abbr.

11-11-07, 21:28	#13 (permalink)
MrMycelium Bud duster Join Date: Aug 2007 Posts: 975	My hair is almost down to my ass, I am doomed. lol __________________ Last night I lay in bed looking up at the stars in the sky and I thought to myself, where the heck is the ceiling.

11-12-07, 14:42	#14 (permalink)
greenweanie Post Whore Extraordinaire Join Date: Dec 2006 Posts: 469	thanks for the observation hippie. i should have noticed that right away my old house - no pets. the gf's house, dog and soon to be a couple of kittens. guess its time i make a larger glovebox for doing larger projects than basic bread tins

11-12-07, 15:23	#15 (permalink)
twoguysupnorth Mycotopiate Join Date: Aug 2007 Posts: 1,597	thanks for the info. well there is no way around a glove box here i bet. a few pets and a dusty old house. i havnt even tried yet, but i was seriously concerned about the dust already. any tips? because there is no way around it.

11-12-07, 15:33	#16 (permalink)
NEPHROSIS Mycotopiate Join Date: Apr 2007 Posts: 233	Good to know, glad I'm bald hehe.

11-12-07, 15:38	#17 (permalink)
scientia Mycotopiate Join Date: Jul 2007 Posts: 375	I have two cats and I really don't have any problems with contams. The house isn't very clean either, and was probably built in the 1800's. It's all about being clean when you need to. I use a glove box to do ALL innoculations, agar work, printing, everything. I also don't let my cats into the room where I keep my fruiting chambers, and still ozium it down before spawning anything. Also, a little bit of bleach in everything helps too. In short, don't get rid of your animals/shave yourself. Those tyvek suits ARE surprisingly affordable though . __________________ -knowledge is power

11-14-07, 06:53	#19 (permalink)
bear Silly Animal Join Date: Aug 2007 Posts: 989	Yeah.. good observation - it seems to agree with the general issues around here.

11-14-07, 13:40	#20 (permalink)
myco-curious Mike O. Kuerias Join Date: Feb 2007 Posts: 68	When inoculating, those are a lot of the same precautions I take. After setting up the environment and while allowing the dense fog of oust to thoroughly settle in the room undisturbed... I do the follow regime. Turn off the HVAC unit for the house, throw a freshly laundered pair of scrubs into the dryer/tumbler and head off to take a shower. I shave, brush my teeth, use mouth-wash and get dressed with the still warm scrubs. I then pull my hair back into a tight-fitting cap, put on latex gloves as well as a set of new tyvek wrist sleeves. I then put on a surgical mask (which is not the same as a dust mask) and a pair of safety glasses. I definitively look like a fool at this point...but I'm ready to enter my now (reasonably) sterilized inoculation environment. --- That being said, once I throw a colonized tray into the fruiting chamber I've been thoroughly neglectful of any form of sterilization procedure - hell the damn thing sits right next to an old deep-freezer that's died on me several months prior, but have only had one case of trichoderma show up so far and that was out of this grow...and I would be damned if the transport into the chamber wasn't something like a hair or whatnot. dust masks are designed to protect you from inhaling rather large particles from the environment, surgical masks are designed to stop your exhaled breath from entering the environment and potentially contaminating a patient. They're not interchangeable... __________________ :shrug: Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected...

11-14-07, 17:53	#21 (permalink)
twoguysupnorth Mycotopiate Join Date: Aug 2007 Posts: 1,597	yeah there is one room in the house where pets arent allowed. i dont think i would stick with it if i had to wear surgical masks and stuff(just out of my realm for things i want to do) a little work isnt bad though. ps, and i am only bald by choice about half the time,lol. ill take the extra measures but if it really turns out to be so hard, i'll leave it to the proffesionals and read about it.

11-14-07, 18:16	#22 (permalink)
OG Royal Grower Philosopher Join Date: Feb 2007 Posts: 109	whats the best way of printing when trich is present? any precautions different from typical prints

11-14-07, 18:23	#24 (permalink)
OG Royal Grower Philosopher Join Date: Feb 2007 Posts: 109	what about choosing a strain because of its ability to grow regardless of contamination? Is printing not an option? is cloning possible still?

11-14-07, 18:30	#25 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,765	no such thing as a contam-proof strain many things are 'possible' yet not advisable. i would not clone from any contam'd tray either. the best option when you know your print is likely contam'd involves transferring to agar, multiple times, to isolate good clean mycelia for lc to spawn , etc.

11-14-07, 20:50	#26 (permalink)
vinz mycotopiate Join Date: Nov 2007 Posts: 1,216	nice one hip il try hair on agar one day if i have time hehe or someone else beat me to it.. __________________ R.I.P. Hippie3. You will always be remembered.

11-14-07, 20:54	#27 (permalink)
mikeg home scientist Join Date: Jul 2007 Posts: 74	i have a walk in closet committed solely for lab purposes since i have four cats. is this fine? i was thinking of getting an ionizer too. i have only went through a bout of contams this week and its a black mold not trich. but i do see cat hairs in my popcorn jars all the time and they colonize fine. have even seen some hairs on casings but i just gently pick it out

11-10-09, 00:34	#29 (permalink)
bmad Mycophiliac Join Date: Mar 2008 Posts: 352	wow heritage that post is two years old.. no one can say you are not doin your research.. Thanks for bumpin it though this is something I didnt know __________________ emotionally crippled narcissist

11-10-09, 02:07	#30 (permalink)
heritage ranch Mycotopiate Join Date: Oct 2009 Posts: 350	plenty of good stuff to read thru here, gona keep me busy for some time

11-10-09, 02:35	#32 (permalink)
August West stumbling Join Date: Feb 2008 Posts: 750	i'm gonna start calling my tricho cereus', hair candles and now, back to the topic. __________________ “How I hate those who are dedicated to producing conformity.” - William S. Burroughs Last edited by August West; 11-10-09 at 02:36. Reason: mas

11-10-09, 06:53	#33 (permalink)
~lw420 Guest Posts: n/a	hip have you got a chance to put ur uv light to it?

11-10-09, 07:11	#34 (permalink)
zodd prone to random outburst? Join Date: Jan 2009 Posts: 1,043	ill keep this in mind thanks

11-10-09, 09:52	#35 (permalink)
~Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 42,765	trich ? no, so far i'm just using the uv wand to clean up foil prints, and my flowhood work area.

11-10-09, 11:17	#36 (permalink)
eatyualive ExoCannibalist Join Date: Jan 2006 Posts: 5,374	uh oh looks like a trich war is brewing. __________________ Click for Eat's Reference

11-10-09, 11:32	#37 (permalink)
copelandiaKidd Void Of Boundaries Join Date: Jul 2009 Posts: 859	wowza. that is a great observation!!!!!!!! I will run some tests myself!!!!!! __________________ "LEARN THE RULES SO YOU CAN BREAK THEM PROPERLY" -The Dali lama "i don't care if it eats my cat, as long as the damn thing fruits"~ prism

11-10-09, 12:20	#38 (permalink)
zodd prone to random outburst? Join Date: Jan 2009 Posts: 1,043	might be worth testing if pasteurised hair is just as prone. just a thought