Microscopy

[Alan Rockefeller]

Psilocybe stuntzii, Seattle, WA, Blimeygrimey collection:

Psilocybe stuntzii, Seattle, WA, Blimeygrimey collection:

Psilocybe species (P. subaeruginosa?), Seattle, WA, Blimeygrimey collection:

Psilocybe cyanescens, Seattle, WA, Blimeygrimey collection:

Gymnopilus luteofolius, Seattle, WA, Blimeygrimey collection:

Gymnopilus sp. gill edge, GGP, CA

Psilocybe subaeruginascens, GGP, CA


Psilocybe subaeruginascens, GGP, CA

Hypholoma sublateratum, Paul Stamets collection

Hypholoma sublateratum, Paul Stamets collection

Gymnopilus luteofolius, Alan Rockefeller flower pot collection, CA

Gymnopilus luteofolius, Alan Rockefeller flower pot collection, CA

Psilocybe zapotecorum, Colima, Mexico, Cactu collection

Psilocybe zapotecorum, Colima, Mexico, Cactu collection

Bluing Pluteus species, Curecat collection, CA

Bluing Pluteus species, Curecat collection, CA

Psilocybe cubensis, Waxingren lawn clippings collection

Psilocybe cubensis, Waxingren lawn clippings collection

Panaeolus subbalteatus, subbedhunter420 collection

Panaeolus subbalteatus, subbedhunter420 collection

Panaeolus subbalteatus, subbedhunter420 collection

Psilocybe ovoideocystidiata - Belmont - Shroomydan collection

Psilocybe ovoideocystidiata - Belmont - Shroomydan collection

Psilocybe ovoideocystidiata - Bethany - Shroomydan collection

Psilocybe ovoideocystidiata - Bethany - Shroomydan collection

[mycobri]

wow. nice work quite a variety too

[little white rabbit]

great pictures

[mjshroomer]

Just wanted to say that that is a great collection of microscopy images of some of the psilocybian mushrooms and related species you just posted here.

Quite impressive.

A great Pictorial of archival materials.

Kudos

mjshroomer

[the_chosen_one]

thank you Mr Rockefeller!
>>>ARCHIVE MATERIAL<<<

[Alan Rockefeller]

Psilocybe cyanescens, Golden Gate Park



Psilocybe cyanescens, Golden Gate Park



Psilocybe cyanofriscosa, San Francisco



Psilocybe cyanofriscosa, San Francisco



Psilocybe mexicana Jalisco, Prankster239 collection



Psilocybe mexicana Jalisco, Prankster239 collection



Panaeolus cyanescens, Prankster239 collection



Panaeolus cyanescens, Prankster239 collection

[Alan Rockefeller]

Psilocybe azurescens, Sporeworks collection



Psilocybe azurescens, Sporeworks collection



Psilocybe villarrealiae, Cactu collection



Psilocybe villarrealiae, Cactu collection

[camMyco]

AllenR, Thanks for taking the time to share that with us. I just got a usb camera for my scope and hope to be posting some pictures soon.

[mycot]

Thanks Allen.
You make lookin at spores so damn interesting.
Love the detail. What magnification you got going on the detailed pics.
I thought only electron microscopy could do shit like that.

[Lazlo]

Quite a bit of bacteria in this one. Thanks for sharing Allen.



Archive Material

[Alan Rockefeller]

Panaeolus cyanescens, Australia, _OttO_ collection





Psilocybe subaeruginosa, Australia, _OttO_ collection





Psilocybe cubensis albino, Australia, RR rescue, _OttO_ collection





Hydnum repandum with methylene blue stain, Whole Foods collection





Psilocybe semilanceata, 1000x:



Psilocybe semilanceata, 1600x: This is the highest magnification I can do with my current setup, the scope is set to 1600x and the camera is zoomed in to 4x, making the total magnification 6400x.







Pluteus salicinus spores, Indiana, 1000x, Godflesh collection



Psilocybe subaeruginascens, auweia 01/26/08 collection

Spores, 1000x







Cheilocystidia, 1000x






Blue/Green stained cheilocystidia can be seen in the next four pics:









Pleurocystidia, 1000x














Psilocybe cyanescens, Fakhface collection

Spores, 1000x



Spores, 1000x, three spores in the middle have their germ pores facing up.



Spores, 1000x, end view



Spores, 2800x

[auweia]

Thanks for posting that here Alan

this section > Psilocybe subaeruginascens, auweia 01/26/08 collection

is from the very same patch as documented in MJ's SEM thread. At the time, there were only two specimens and I had no idea if those two were the only ones as this was a new find. It should be the same species as the one I found in Richmond two years ago, one of them being in the SEM that I sent to MJ

So what I mean is that your microscopy should come close to one of those two SEM. I notice that the spores look like lemons, as opposed to cyans and friscosas, which are more circular and very similar to each other

Psilocybe subaeruginascens, auweia 01/26/08 collection

http://forums.mycotopia.net/wild-mus...ofriscosa.html (SEM of Ps. cyanescens and Ps. cyanofriscosa)

I can show you the actual mushroom this came from



That was back in January. About a month later, the rest of the patch came up. This is what they really look like



as far as anybody knows, this is only been seen 4 times around the San Francisco Bay Area, with the first being in 2004, in GGP

[kocos]

Gorgeous

[Alan Rockefeller]

Psilocybe villarrealiae spores, 1000x, Prankster239 collection






Psilocybe caerulescens spores, 1000x, Prankster239 collection








The biggest difference I see in the spores between the two species is the color. It is noticeable both looking at the print and through the the microscope. The P. villarrealiae spores are a reddish brown while the P. caerulescens spores are closer to a charcoal gray, just barely if at all brown. After looking at the P. villarrealiae spores in the microscope for awhile, the P. caerulescens looked like chunks of charcoal by comparison.

[the_chosen_one]

beautiful!

mr rockefeller we need to cut you loose on some albino hybrid spores

[warriorsoul]

Nice thread Alan!
Do you have any baeos to compare to my collection?

[Alan Rockefeller]

> mr rockefeller we need to cut you loose on some albino hybrid spores

Ok if you mail them to Illinois I will look at them.

> Nice thread Alan!
> Do you have any baeos to compare to my collection?

Unfortunately I do not.

[Alan Rockefeller]

Psilocybe ovoideocystidiata (Belmont) - Shroomydan collection

Scanning electron microscopy by Scout24

1800x:


3000x:


10000x


Gymnopilus luteocarneus, Santa Cruz Fungus Fair 2009

Spores moderately roughened, inequilateral, dextrinoid, no germ pore, 4.5 â 5 Ã 7 â 8 micrometers.



The spores turned from golden yellow to brick red after being in Melzer's reagent for an hour.



Gymnopilus lutescens

Spores roughened, 7 - 8 x 5 micrometers. These spores are rougher than the spores from G. luteocarneus.





Spores mildly dextrinoid



Gymnopilus flavidellus

Spores 6 â 7 (8) x 4 â 4.5 um, minutely roughened



Spores immediately dextrinoid



Tuber oregonense (oregon truffle), Ice House Shaman collection

Notes: Spores measure 26 - 52 x 24 - 28 micrometers (not counting the ornamentation of course).

The spore sacs that have just two spores have much larger spores than the sacs that have 3 or 4 spores. The spores are evenly distributed throughout the inside of the truffle.

[Fresh Brewed]

Those scanning electron micro pictures are awesome! thanks!

[Alan Rockefeller]

Those scanning electron micro pictures are awesome! thanks!

I agree, but I don't have access to one anymore.

If anyone has access to an electron microscope and can take pictures of random samples that I send, please PM me!

[Phungivore]

wow is all i can say. major kudos

[Shroomette]

Those are amazing Alan. Were any of those taking from live cultures? or just spore prints.

[Om shanti]

Amazing thread that deserves a BUMP. Very interesting, and beautiful photos!

[the_chosen_one]

indeed.

last call.