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Old 07-20-09, 09:28   #51 (permalink)
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i guess after reading my post what i meant to say is, how much H2O2 to 1/2 pint cake, should i use per amount of bulk sub. i'm kinda unclear about this from the posts above, plus it's pans not cubies, but fully colonized.
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Old 07-20-09, 10:24   #52 (permalink)
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what volume of substrate are you thinking about using. the above volume? for pans id say slurry about one turkey tin volume substrate per 1 pf cake. this in a large tub may not completely fill the bottom. if your intentions are to have a tub to do so. then you may want to get a smaller tub. or you can use a tray. for pans ive always used thoses 4" deep cat litter trays. and i only usually make the pan subs 2" deep maybe with a small 1/4" casing layer on it. i think a turkey tin would fill one of those cat litter trays a few inches for the volume of substrate.

maybe just follow the 1 pint liquid to 1/2 pint mycelia for the slurry. you probably want a smaller volume of substrate with pans as the mycelia is brittle.

and the slurry itself may not even be as muddy at the cube mycelia. only know when you try this.

but if your balsy. try 2 turkey tins volume like the above ratio. although for first trial. id probably start off with a lower volume so you don't waste if it contams. then work your way up volumes to find out where that balance is with the one pf cake volume of mycelia. there has to be some kind of volume limit for the amount of water. we just have to figure it out.
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Old 07-20-09, 10:25   #53 (permalink)
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The cubes will still get nutrition from the substrate and grain is very very nutritious to cube, so maybe if you slurry it like that it will give all the necessary concentrated nutrition, and also make the inoculation points more similar to a high grainspawn ratio. I dont know lewlz

Maybe supercake can offset some of the loss of concentrated grain nutrients
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Old 07-20-09, 10:37   #54 (permalink)
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The cubes will still get nutrition from the substrate and grain is very very nutritious to cube, so maybe if you slurry it like that it will give all the necessary concentrated nutrition, and also make the inoculation points more similar to a high grainspawn ratio. I dont know lewlz

Maybe supercake can offset some of the loss of concentrated grain nutrients
yeah good idea. thats why i tossed the supercake in there.
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Old 07-20-09, 13:48   #55 (permalink)
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cant wait to see the ending result in this.
 
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Old 07-21-09, 06:23   #56 (permalink)
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Yesterday I slurried 2 x 0,35 l brown rice cakes into around 10-12 l of straw in a tray. Some growth is visible already.

I'll post the pics when I succeed .

I couldn't fit the blender and a jar into my glovebox so I just did it in open air, just clean. I mean - what's the difference whether you make a slurry or just spawn it if you're doing it in open air anyway?
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Old 07-21-09, 12:27   #57 (permalink)
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finally got pins today.
7-21 posted in 1st post.

below is amazon and ape. a few days in.
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Old 07-21-09, 12:27   #58 (permalink)
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Yesterday I slurried 2 x 0,35 l brown rice cakes into around 10-12 l of straw in a tray. Some growth is visible already.

I'll post the pics when I succeed .

I couldn't fit the blender and a jar into my glovebox so I just did it in open air, just clean. I mean - what's the difference whether you make a slurry or just spawn it if you're doing it in open air anyway?
i think it should be fine as long as your clean about it. next time, you can use clear 50 gallon or larger lawn bags. should give you plenty of work area.

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i guess after reading my post what i meant to say is, how much H2O2 to 1/2 pint cake, should i use per amount of bulk sub. i'm kinda unclear about this from the posts above, plus it's pans not cubies, but fully colonized.
no h202. distilled water only. h202 isn't necessary.
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Old 07-21-09, 12:57   #59 (permalink)
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that was fast lol i still see a bunch of strawnet
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Old 07-21-09, 13:16   #60 (permalink)
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that was fast lol i still see a bunch of strawnet

pic was uploaded to the first post. sorry.tex strain pins faster than most strains when it has the right conditions. usually if the tex strain isn't pinning very fast its most likely your moisture content is far too dry.
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Old 07-21-09, 18:30   #61 (permalink)
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How about tossing some sterilized water in a food processor with a pf cake or two and making some real mud for a smaller tub say maybe a 30 qt?
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Old 07-21-09, 19:33   #62 (permalink)
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How about tossing some sterilized water in a food processor with a pf cake or two and making some real mud for a smaller tub say maybe a 30 qt?
a chance you have small food particles stuck in it. with the blade assemblies and everything like that. you are assured that you have sterility. and that your not wasting your time.

if your going to try it. make sure you dish wash your food processor. then probably wipe down everything on the inside with some 90 percent isopropyl.
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Old 07-21-09, 19:35   #63 (permalink)
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My food processor has never ever had food in it
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Old 07-21-09, 20:01   #64 (permalink)
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My food processor has never ever had food in it

id say then that you might be in luck! try it out. but still clean it well. and might just want to keep that processor for this purpose only.
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Old 07-21-09, 20:20   #65 (permalink)
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Nice! I bought it for spawning purposes only. I don't have a dishwasher at this time, she's out of town at the moment
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Old 07-21-09, 21:19   #66 (permalink)
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Nice! I bought it for spawning purposes only. I don't have a dishwasher at this time, she's out of town at the moment

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Old 07-21-09, 22:51   #67 (permalink)
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Old 07-22-09, 11:24   #68 (permalink)
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Before using the food processor, I'd do an Iodophor soak/rinse of all the parts and I'd avoid isopropyl alcohol since it MIGHT etch and weaken the plastic. You can get Iodophor at homebrewing supply shops.

If the alcohol makes the plastic foggy or scratchy, it's eating it. Iodophor is just iodine and water and is neutralized w/ mild heat (plus works better than alcohol). I dip my trays in it before adding substrate and then hit the trays with a shot from my steam cleaner to neutralilze the iodine.
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Old 07-22-09, 11:28   #69 (permalink)
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And long time no see, Bobcat! Hope life's been treating you well...
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Old 07-22-09, 21:02   #70 (permalink)
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updated pins pics at 1st post.
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Old 07-22-09, 22:42   #71 (permalink)
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Old 07-23-09, 05:14   #72 (permalink)
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Hell yeah!!

Congrats, man!

update: 3 days into spawning and my tray looks truly promising, all fuzzed up throughoutly even though the temperatures haven't been perfect. I'll try to post some pics in a couple of days but from what I can observe the biggest advantage of it is that nearly whole substrate gets covered in the spawn. The growth seems to be more spread but a little lighter comparing to traditional spawning, nevertherless very promising method. Unfortunately I can't compare two identical trays. I'll make some more trays today probably with plain coir.
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Old 07-23-09, 09:40   #73 (permalink)
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hey eats, i have some pix to upload of the pans.....BUT.......I don't know how! could you help me out. the "slurry in a hurry" seems to be good. within 12 hrs i could see regrowth. but i don't know how to post it! i have a 24 hr, and a 48 hr pic, tonight i'll take a 72 hr pic too. PM if you like or put it in the thread......thanx
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Old 07-23-09, 13:15   #74 (permalink)
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hey eats, i have some pix to upload of the pans.....BUT.......I don't know how! could you help me out. the "slurry in a hurry" seems to be good. within 12 hrs i could see regrowth. but i don't know how to post it! i have a 24 hr, and a 48 hr pic, tonight i'll take a 72 hr pic too. PM if you like or put it in the thread......thanx
allright. click reply to post a reply. below the window you will see a button that says manage attachments. click that. then upload the pictures. then if you want to format it just let me know or ill fix it for you. then hit submit reply.


glad you guys are seeing good results. yeah at first i thought it was strange looking the way it was growing. kinda light. but then after day 6. it just fuzzed up and turned super rhizo! just wait till that 7th day and it makes a big change from day 6 to day 7. its quite impressive!

the pins are looking a little slim today. so maybe the yield isn't going to be so good. but still better than a pf cake!
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Old 07-24-09, 12:15   #75 (permalink)
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pan cyan slurry.....take 1

ok here are a few pix using eat's slurry in a hurry tek but with pan cyans. the sub is an unmeasured amount of aged HPoo, course verm, lime, calcium, and gypsum, past. in the oven at 170 for 4 hrs. i then slurried 1 PF 1/2 pint jar to 1 pint sterilized distilled H2O. (I believe this was too much water though, at least for pan myc.) then a 24 hr shot of the sub, wich showed recovery within 12 hrs, but i didn't take a shot of that. then a 48, and a 72 hr pic respectfully. i'll get more pix as it changes. there doesn't seem to be too much of a difference between day 2 and day 3, so i'll only send pix that have more change in growth.
Attached Thumbnails
liquid-spawn-slurry-spawn-slurry.jpg   liquid-spawn-slurry-spawn-just-poured.jpg   liquid-spawn-slurry-spawn-24-hrs.jpg   liquid-spawn-slurry-spawn-48-hrs.jpg   liquid-spawn-slurry-spawn-picture-012.jpg  
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Last edited by pvacant; 07-24-09 at 17:07. Reason: that was water H2O, not peroxide H2O2 oops!
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Old 07-24-09, 13:15   #76 (permalink)
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How about tossing some sterilized water in a food processor with a pf cake or two and making some real mud for a smaller tub say maybe a 30 qt?
bingo (lightbulb EXPLODES in caps chamber of secrets )
im doin an outdoor show with some asian edible from my bro in law.
got a pint of spawn to extend. thanks man. and thx king strawnet i forgot about ur new tek!
thanks a million! this tek is good for mosdt any variety of boomers! not just funny kinds

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Old 07-25-09, 18:07   #77 (permalink)
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nice pvacant. you adding a casing to that?

id say after day 2 to day 3 there wasn't much difference until closer to the end of the week for me.

here is some updates.

screwed up the strain. this is a 3rd generation 2nd flush clone off year old tasmanian material. so its really old. and stretched out. but it still worked!

either way, its fruiting now. had some things come up so the tubs had to be moved and the light was much further away. thats why everything is at an angle lol...
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Old 07-27-09, 12:37   #78 (permalink)
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yeah man i added a casing to it this morning (monday) before work. i however got caught up in a P.torch trip and lost this weekend to oblivion...... well i'm back on track. i'll post some pix when they are ready....but for what it's worth, i belive this tek is OK, and just fine for pan cyans. the myc was a little thin, but recovered quickly, and continued to grow, without any contams thus far.... sorry that i'm a degenerate and couldn't post any other pix.....but MAN was that P.torch awesome !!!!!!! i'll get back as soon as it grows through the casing layer (1/8 - 1/4'' of peat, verm, sand, lime, and oyster shell) until then
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Old 07-27-09, 22:49   #79 (permalink)
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so for 1 pf cake. and being a 3rd generation 2nd flush clone off more than year old material. im impressed!
Attached Thumbnails
liquid-spawn-slurry-spawn-tasslurrypins3.jpg   liquid-spawn-slurry-spawn-tasslurrypins4.jpg   liquid-spawn-slurry-spawn-tasslurrypins5.jpg   liquid-spawn-slurry-spawn-tasslurrypins6.jpg   liquid-spawn-slurry-spawn-tasslurrypins7.jpg  
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Old 07-28-09, 05:24   #80 (permalink)
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Old 07-28-09, 12:32   #81 (permalink)
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those are curly fruits!!!!
why is that? do they resemble the clone, or was it environmental, or the tek? what do you think? none the less, beautiful!!!
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Old 07-28-09, 15:52   #82 (permalink)
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those are curly fruits!!!!
why is that? do they resemble the clone, or was it environmental, or the tek? what do you think? none the less, beautiful!!!

yes took them out of the light and out of the fan. this strain will do that at times. usually if co2 builds up. but some of the side holes of polyfil were taken out during fruiting. but the bins had to be kept in low light low airflow area due to life happening. light source kept switching up as well.

some of the fruits are 15" tall. so far from eye judgment. the flush is about 4z. filled the entire dehydrator.
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Old 07-28-09, 16:02   #83 (permalink)
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Eats those tubs still look pretty brown! Did I miss something and you cased them or do you expose them to fruiting conditions before the top is 100% colonized? I have a mini 3qt monotub that I exposed to fruiting conditions when the top layer of sub was around 60-70% colonized and it is pinning quite well while still colonizing the top layer. I think the top will be colonized before I harvest. It was a little experiment of mine. What are your thoughts on this? on your oven pasteurization method by the way, I don't think I will ever go back to a fucking pillowcase again!
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Old 07-28-09, 16:08   #84 (permalink)
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Eats those tubs still look pretty brown! Did I miss something and you cased them or do you expose them to fruiting conditions before the top is 100% colonized? I have a mini 3qt monotub that I exposed to fruiting conditions when the top layer of sub was around 60-70% colonized and it is pinning quite well while still colonizing the top layer. I think the top will be colonized before I harvest. It was a little experiment of mine. What are your thoughts on this? on your oven pasteurization method by the way, I don't think I will ever go back to a fucking pillowcase again!

which tubs? the pics above of the amazon tub colonizing were not birthed until it was 100% colonized. im not sure which pictures your referring to. they were all birthed once colonization was complete. nothing was cased. just substrate.


cool. yeah i don't so much like to expose the substrate to light and fruiting conditions until its 100% colonized. i suppose its personal preference. some ppl have good results exposing early. ive just never had good results trying it.

also slurry in a hurry tek was the nickname for tv's how to colonize jars in 3 days. slurry tek. slurry spawn tek. whatever you wanna call it. liquid mud spawning tek lmao...
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Old 07-28-09, 16:14   #85 (permalink)
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Ok i get it, I missed the part where I was supposed to go back and look at the updated pics in the first post. Im not always on top of my game lol. Im trying like hell to only check my tub every 4 days or so to keep light off it.....My first monotub though, it's killing me lol.
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Old 07-28-09, 17:22   #86 (permalink)
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Ok i get it, I missed the part where I was supposed to go back and look at the updated pics in the first post. Im not always on top of my game lol. Im trying like hell to only check my tub every 4 days or so to keep light off it.....My first monotub though, it's killing me lol.

sorry about the confusion. once the 2nd page started it would no longer allow me to merge new posts to upload the pictures. might just upload em to post one so ppl don't have to scroll the entire thread.
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Old 07-28-09, 17:25   #87 (permalink)
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sorry about the confusion. once the 2nd page started it would no longer allow me to merge new posts to upload the pictures. might just upload em to post one so ppl don't have to scroll the entire thread.
Good plan, I'm just a little dense sometimes lol.
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Old 07-30-09, 16:13   #88 (permalink)
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im thinking like this. any newb who has pf jars. can do bulk now instead of just pf jars. seems to be grain is one of the hardest things to grasp when your new at working with the fungi. anyone can mix up some subs with water. put it in a turkey tin. then cook it at 170 for 2.5 hours. then just slurry up the substrate in a tub. and mostly anyone can make pf jars. this is really a tek that i think is great for beginners.

i can slurry a tub and not have to complicate shit with grain jars or even really being clean about it. really its just semi clean. its like skipping a step. and in the instance of my last 2 months of contams. it actually helped get things going waiting on slow spawn to colonize.

i always use pf cakes anyway. i do like 10 masters from one clone syringe via 9er tek. then take 1 of those and slurry it into anywhere from 10 to 100 quart jars of grain. but usually im only doing 10 jars at a time. so really its a waste of that pf cake if your slurrying it. and if you just scrape it with a fork via g2g. your waiting more than a week for your grain jars.

most of the time im tossing out half the pf cake. lately ive been lowering the water content of the slurry so its more muddy in the pf jars. this works well. but can slow growth a little bit in the jars. which isn't bad if the average colonization time for the quart jars is 3 days. also those pf masters are kept in the fridge up to a year. and it takes up less space than quart masters. so anytime i need to slurry quarts or a substrate. i can go right to it. no down time at all. within 3 days have quarts or just slurry that pf cake into a tub now if your lazy and don't like waiting the extra 3 days for grain to colonize. or if your lazy and don't even want to prep grain jars. to me, prepping grain jars is like the most time consuming aspect of this hobby. and you don't have wait to prep the grain jars.

now a days you can do grain without a pressure cooker. using antibacterial grain soaks and then steam the jars for an hour and a half in a pot. the growth doesn't look as vigorous to me, but it still works.

its made pressure cookers obsolete for the beginner home cultivator. now that might be a different story for the bulk cultivator. this is just a simple way of making a pf cake to bulk in a decent time frame. i was looking at it like one pf cake might get me a few grams of yield. while this tub might get me a little more lol..

the idea was that lets say one has a high trich count in the air. and everything is contaming beyond belief as far as grain goes. but, you have a few pf cakes laying around. then you can maximize those pf cakes with what little you have if you have doubts about your grain.

trich was killing me the last 2 months. i tossed out 145 quarts of wbs. ive not done anything different for years and i have upwards of like 6 total jars per year i toss out to contams on the normal flux of things. making 10 quarts of grain a week.

for some reason the clone work and clean work i did was fiine but i tried everything. i tried so many different spawning methods. nothing worked. all wbs grain contamed. nothing else contamed. nothing in any tubs or clones. just the grain. once i switched to popcorn it was 100 percent no contams. but popcorn is expensive.

so i just tested this waiting on spawn to colonize. really just a fun little experiment. meanwhile, dry yield is only 72 grams dry. 2.5 z. which is not bad for 1 pf cake! which is usually my worst yield on a bulk tub that is too dry or some other factor causing issues.

not as good as i wanted the results to be. but this is just a test run. the material being used was rather old. so that accounts for something.

and im tweaking these new tubs. i can care for this next trial tub more bc life isn't in the way. the last one kinda looked like sphagetti....lol..

as far as bulk goes. ill stick with grain jars for the bulk tubs to maximize yield. but in a desperate situation i might use this as a backup plan as i just did. hopefully some fresh genetics might help increase the yield. as far as i can see. its comparable to about 2 quarts of spawn in a bulk tub. but its only 1 pf cake.


might be an interesting test to put a casing layer on this amazon tub. but im just too lazy to pc a casing for an hour.
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Old 07-30-09, 18:37   #89 (permalink)
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hey now that is impressive i gotta say. Great experimentationization eats.

Maybe try to compare the yield, to a couple quarts of slurry from grain? That would be interesting as well
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Old 07-30-09, 20:18   #90 (permalink)
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hey now that is impressive i gotta say. Great experimentationization eats.

Maybe try to compare the yield, to a couple quarts of slurry from grain? That would be interesting as well
im worried with wbs grain at least that some of the grain won't colonize all the way through and the inside exposure of the grain might contam. but it might work. never know till you try.
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Old 07-31-09, 12:38   #91 (permalink)
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this also seems to be working well for pan cyans. just cased a shoebox, 2 days ago, from one half pint pf cake (with hpoo), slurried, and it's been growing a little thin, but growing without contams. it completely grew into the casing in 2 days. so i would say the mycilium is strong and healthy. not effected too much by the slurrying IMHO. will post pix when it fruits.
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Old 07-31-09, 12:41   #92 (permalink)
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this also seems to be working well for pan cyans. just cased a shoebox, 2 days ago, from one half pint pf cake (with hpoo), slurried, and it's been growing a little thin, but growing without contams. it completely grew into the casing in 2 days. so i would say the mycilium is strong and healthy. not effected too much by the slurrying IMHO. will post pix when it fruits.

awesome!

the amazon tub i have is not pinning yet. also i had a prior tub of it not fruit at all. i think i need to add a casing. but damn im way too lazy to cook it....
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Old 07-31-09, 17:49   #93 (permalink)
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im worried with wbs grain at least that some of the grain won't colonize all the way through and the inside exposure of the grain might contam. but it might work. never know till you try.
Well it didn't contam for me (I used rye, or brown rice or whatever), it was going pretty well until it stalled so I kinda changed my methods on this slurry-tray that I made.
But that's another story - I think I fucked up the straw, I chopped it so well that the whole substrate got too stiff and heavy and wouldn't colonize properly, but that's my fault.

But grain shouldn't be a problem. If your making a smaller tub, high slurry-spawn ratio should be a killer option to go with. It will colonize really fast.

All just clean, in open air and so on. It's just like spawning but with a little twist.
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Old 08-03-09, 12:54   #94 (permalink)
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Thumbs up

ok eats, or anyone else who cares,

i got mature fruits on sunday (08/02), off of the pan cyan slurry in a hurry tek (i think it was 2-3 days in the fruiting chamber). nothing great, about 1/4 of it flushed... BUT, this morning, i couldn't even count the amount of pins, (and Hyph knots) comming up......i have a pic, but i'm having hella computer problems, and it's taking about 5 minutes to do ANYTHING!, so if i can get this fixed i'll upload the pix. however the goal was to see if this tek will work with pans, and the answer is YES. i would however add, that because the myc is rather thin, compared to cubies, the water ratio should be ......perhaps (IMO)......1/2 pint BRF - 1/2 pint H2O, or a 1:1 ratio of slurry. none the less Eats, you created a very easy way to extend pan myc, and obviously cubie myc, without grains! My vote is a YES! thanx
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Old 08-03-09, 15:02   #95 (permalink)
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ok eats, or anyone else who cares,

i got mature fruits on sunday (08/02), off of the pan cyan slurry in a hurry tek (i think it was 2-3 days in the fruiting chamber). nothing great, about 1/4 of it flushed... BUT, this morning, i couldn't even count the amount of pins, (and Hyph knots) comming up......i have a pic, but i'm having hella computer problems, and it's taking about 5 minutes to do ANYTHING!, so if i can get this fixed i'll upload the pix. however the goal was to see if this tek will work with pans, and the answer is YES. i would however add, that because the myc is rather thin, compared to cubies, the water ratio should be ......perhaps (IMO)......1/2 pint BRF - 1/2 pint H2O, or a 1:1 ratio of slurry. none the less Eats, you created a very easy way to extend pan myc, and obviously cubie myc, without grains! My vote is a YES! thanx
yea id agree with your ratio. also id even say with cubes you could lower it too. nice job man thanks for testing it!
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Old 08-03-09, 19:22   #96 (permalink)
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great tek indeed! but i dunno.. i find it easier to prep rye than to prep PF jars.. all that rice grinding, and getting the moisture content right can sometimes be tricky..
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Old 08-04-09, 08:06   #97 (permalink)
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great tek indeed! but i dunno.. i find it easier to prep rye than to prep PF jars.. all that rice grinding, and getting the moisture content right can sometimes be tricky..
I just buy brown rice flour at the health food store, mix w/ verm and water, squeeze to test the sound and see how much water drops out, add more verm or water to make it sound and feel just right (that's how I adjust moisture content), jar it up, PC it, and go. I'm not so concerned about having the highest quality rice flour since it's only a step along the way, but for fruiting off BRF jars directly, I would consider grinding my own rice.

I use an electric mixer to speed it all up even more, so I can make a dozen BRF half-pints in 20 minutes plus an hour in the PC. If doing a multispore grow, I get a dozen colonized jars two weeks later, and up to 120 colonized quarts 4 days after that (or 240 quarts in 7-8 days -theoretically-). 120 quarts ready to spawn only 18 days after starting from spores ain't bad IMO...
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Old 08-06-09, 20:33   #98 (permalink)
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well not the best flush. but still 2nd flush

the amazon tub was stalling out so a casing was added. it happened on another flush with regular spawn as well. probably an old print.
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Old 08-07-09, 09:27   #99 (permalink)
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2nd flush is coming along. appears the yield is going to be bigger than 1st flush.

these are some monsters. off one pf cake!

now the pics might be deceiving. the fruits are hollow stemmed. not as dense as usual.
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Old 08-09-09, 18:40   #100 (permalink)
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2nd flush 81 grams dry.
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