petris

Cheshire_Kat · 11-28-06, 14:19

Took some pictures of my petri culture of spores. They are AMZ on MEA.

I want to move a sample to another dish. There are 2 spots of contamination on the plate. It's been in the fridge undisturbed since before break.
Here's my question. The mycellium isn't very rizomorphic. It's more of a cottony growth that sticks up from the agar. Would I be able drop a drop of peroxide ontot he dish and take a sample from the drop to transfer to another plate?

Cheshire_Kat · 11-29-06, 13:16

no suggestions?
Does this even look right?

lost_onabbey_rd · 11-29-06, 13:36

just use an exacto knife or something similar to take a sample from the edge opposite the contam..
you might wanna do some peroxide agar for the next transfer so that none of those contam spores germinate on the next set..
i'd let that grow out and transfer one more time to make sure it's clean.. then you should be good to go
lost

dial8 · 11-29-06, 14:06

Yeah, no need to drip h202 on the piece to be transferred.

You may have to transfer more than once.

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11-28-06, 14:19	#1 (permalink)
Cheshire_Kat Mycophage Join Date: Oct 2006 Posts: 103	petris Took some pictures of my petri culture of spores. They are AMZ on MEA. I want to move a sample to another dish. There are 2 spots of contamination on the plate. It's been in the fridge undisturbed since before break. Here's my question. The mycellium isn't very rizomorphic. It's more of a cottony growth that sticks up from the agar. Would I be able drop a drop of peroxide ontot he dish and take a sample from the drop to transfer to another plate? Attached Thumbnails

11-29-06, 13:36	#3 (permalink)
lost_onabbey_rd The Lost Join Date: Apr 1972 Posts: 1,359	just use an exacto knife or something similar to take a sample from the edge opposite the contam.. you might wanna do some peroxide agar for the next transfer so that none of those contam spores germinate on the next set.. i'd let that grow out and transfer one more time to make sure it's clean.. then you should be good to go lost __________________ Plant a seed, It will grow, So it's been, Sow the show To think outside the box, sometimes it is nessecary to step, outside the box

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11-29-06, 13:16	#2 (permalink)
Cheshire_Kat Mycophage Join Date: Oct 2006 Posts: 103	no suggestions? Does this even look right?

11-29-06, 14:06	#4 (permalink)
dial8 Un Undead ***er8tor Join Date: Jun 1976 Posts: 3,183	Yeah, no need to drip h202 on the piece to be transferred. You may have to transfer more than once.