PE Clone

[scanman]

I tried to clone my biggest PE fruit.I didnt really follow no tek to the T.What i did was first made some no PC LC.Then i took the shroom and wiped it down with 91% alchol,then i cut around the shroom and took 2 pieces out of the middle and put them in the LC i had ready.To me it looks great so far.I didnt put nothing in the jat to cut up the myc.Im not for sure what im going to do to break it up.Anyway here is a few pics of the clone.

[feralronin]

Looks good so far. Good luck scanman good vibes your way.

[Phidell]

Looks very nice.

[Hippie3]

wiping it with alky was a bad idea,
use povidone iodine instead, water-based
povidone-iodine .

[livewire420]

nice bro

best lids ever.


pe clone saved from brf to wbs.


won't be long til i make another lc + a back up wbs clone jar.

[livewire420]

when you plan on testing it out?

just shake it.

Originally Posted by scanman

I didnt put nothing in the jat to cut up the myc.Im not for sure what im going to do to break it up.

[Freaky]

Shaking works well to break it up. Also, if you draw your syringe in your glove box, you can do a suck/shoot method to help agitate it and break it up.

Blunt end needles work great too, if you want to cut your tips. Careful though, they will leak.

Looks great, and I always dipped in peroxide, then dumped in the jar. Iodine like Hippie mentioned is great also.

[scanman]

I injected 2 cases of half pints BRF jars today.I hope it wasnt cotamanated.Ill let everyone know in a few days.

[blackout]

I have never had any success cloning direct into LC. I have cloned well into jars of grain, got me thinking I should add a small bit of grain to a jar and clone, then use this as a master, shoot in LC or just water to wash the myc off and then suck back out. I heard myc will take to wood too, even non-wood lovers. So a mix of grain and wood might be best as the master, then let it dry out a bit and store in the fridge, I would bet it would survive longer than a regular LC, and be reusable.

Hippie is right about the alcohol. It could be done in a glove box and allowed to dry a bit, if there is enough LC then it should be diluted enough not to cause trouble. But if in a glove box I would just cut into the stem and take some core and not dip in anything.

[livewire420]

might be your problem with cloning

Originally Posted by blackout

take some core and not dip in anything.

always dip in at least a 3% peroxide

http://archives.mycotopia.net/discus.../5/147159.html
and yes you can do a clone to grain master jar kinda like fahtster. after the grain colonizes shoot some sterile water in on it. then shake/swirl. afterwards draw the water back up into another sterilized syringe. knock up some jars



hope them jars worked out for you scan

[lost_onabbey_rd]

if your gonna take a sample form the inner stem there is no need to dip in anything, as the core will be clean and sterile, provided you tear the stem, don't cut it, if you cut it your blade can push comtams on the outside to the clean inside part. tear it in half length wise like your pealing a banana.
lost

[blackout]

Originally Posted by livewire420

might be your problem with cloning

always dip in at least a 3% peroxide

Many say the peroxide slows down growth, and if from the centre it is sterile, no need to clean it. My attempts did not contaminate, just never took off at all. I did try peroxide before going to grains, and did find it slowed growth a little, my undipped stems too off right away.

Nice guide you linked to, lovely pics.

The other technique is "coring", which requires little sterile technique. Simply get a syringe with water or air in it. Get your shroom and wipe the outside, now pass the needle through to take out a core section from it, now inject this into your grains. I planned on doing this to a trip to holland. There are some different commercial strains in holland not available online. I could travel with a sterile syringe and a tiny pot of sterile grain. Core a section and toss the syringe away. I expect commercial growers spend a lot of time developing a good strain and it would be nice to get a clone of it. Coring with sclerotia is especially easy since it is dense and easier to get a core from.

[livewire420]

ya i know about that seen it on the shroomery in fact a mod done up his version here
http://forums.mycotopia.net/holding-...n-dummies.html (Clone Selection and Agar Propagation for Dummies)

[scanman]

the clone turned out to be good.all my jars are over 90% done.they was injected 6-17.in a few days ill be birthing them all.ive had great grows so far by cutting my cakes up and making a casing out of them.i think ill stick with that with these clone jars allso.

[eatyualive]

nice scanman. would your conclusion be that lc's rock?

i think they do...

[Freaky]

That mycellium looks good and healthy! Nice cloning!!!!

I for one can't wait for the fruiting

[scanman]

Originally Posted by eatyualive

nice scanman. would your conclusion be that lc's rock?

i think they do...

oh ya,LC rocks.i have another dozen BRF jars allmost done of PE LC.i injected them on 6-11.6 of them have been birthed 2 days ago,the rest will be anyday now.id guess the PE LC,and the PE CLONE will be birthed the same time.Id like to say thanks to everyone,especically livewire420for helping me out with the cloneing project.

[scanman]

since the last post ive done allot with PE LC,clone,and wbs.the first pic is of 3 casings,the first is lc brf cakes cut up and cased with verm-core,the second two are LC wbs mixed with verm-core nothing on top.The 2nd and third pics are LC brf cakes cut up and cased with verm-core.The 4th pic is of two casings,the first is lc cakes and the other is clone brf cakes cut up and cased.The 5th pic is of clone cakes cut up and cased.The 6th pic is 3 jars of wbs LC.The 7th pic is of 8 half-pint jars fully colonized of pe clone.The 8th pic is of 9 pint jars 90% colonized.The 9th pic is a JAR OF pe lc that was started a wek ago.there are 5 other jars just like it.Yes you can say im PE crazy

[scanman]

here are a few pics of my pe clone.it looks like there might be some biguns.

[Lookyhere]

Pictures 3 and 4 look like you're going to have some very nice pinsets. Pictures 1 and 2 look like there is a bigun in the making. I can't wait to see this in a couple of days. I can't believe I haven't seen this thread yet.

[livewire420]

looking good bro

[Sideshow]

I'm ready to see a good flush outta this....
Go scanman!

[Freaky]

Excellent clone Scanman!!!!!!! Definitely gonna have some big fruits in there!

[scanman]

Heres some pics of three different PE lc made from print.the first two are made from 3 half pint BRF cakes cut up then cased with verm-coir.the 3rd is PE lc put in WBS then mixed with verm-coir.the 4th pic is just a pic showing the pins growing off the wall of the bowl.

[Freaky]

That is a beautiful strain you have of PE Scanman! The fruits aren't gnarly at all like most of the PE's I've grown.



Are you gonna clone any from those clusters? You seem to have the hang of the cloning

[scanman]

i was thinking of cloning the one in the 2nd pic on the bottom right.that looks like a good one to clone.

[livewire420]

this looks like a nice cluster to clone from

[Freaky]

Yeah, that one hasn't even busted its veil yet. You could also see which prints the best, if at all and clone a printing fruit.

[scanman]

Originally Posted by Freaky

Yeah, that one hasn't even busted its veil yet. You could also see which prints the best, if at all and clone a printing fruit.

Thats brings me to a question.ive had two fruits print from my last pe grow.the only problem was they where small shrooms.i was thinking about cloning the next printable shroom i get no matter how small it is so i can see if i can get a bunch of prints.on that project i wouldt care about the size of the shrooms just that they opened up to print.Do you think that would work to get fruits that print?

[Godflesh]

Yo Scanman! Just an idea, but I think you would get a better end result if ya put a verm layer on that bowl! "the 4th pic is just a pic showing the pins growing off the wall of the bowl." Being seed, verm would help even out the next flush,IMO.

Nice clone!