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    Old 08-04-08, 15:11   #1 (permalink)
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    Panaeolus.. dunk or no dunk

    Some of us now how fragile the Pan mycelium is right...?!

    Well..,when someone ask the question.."should I dunk my Pan casing after a first flush",then most of the time we say ..no just water it real good ,with a spray bottle or something like that...

    Now,I have found out ,that a dunk really give's a better pinset then just spraying !
    I have only tried it a couple of times with a multispore casing,but I'm aboud to do an experiment with three new casings.
    I'm gonne use clones this time and I will make 3 different casings...

    The first casing will be one that wil not get a dunk ,but just be sprayed with a bottle.
    The second wil be dunked right Before the first flush.
    And the third will be dunked right AFTER the first flush

    It's an ongoing project ,so it will take some time,and you have to check this tread from time to time to see how it's going ,if you're interested.

    I know a couple of you guys already dunk Pans casings and I have seen good results,but I have seen no real comparisons posted so far...

    The spawn jars are still in the incubator,but I will show pictures of every stage of this project,if I can...

    Here's the only example I have so far of a failed casing,that I dunked (after the first flush),because I had nothing to lose...
    Before the dunk it had almost no pinset,..see what happened !

    Attached Thumbnails
    panaeolus-dunk-no-dunk-sandoze-multispore-2nd-flush-dunk-001.jpg  panaeolus-dunk-no-dunk-sandoze-multispore-2nd-flush-dunk-002.jpg  
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    Last edited by spacecake : 08-23-08 at 19:29.
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    Old 08-04-08, 20:32   #2 (permalink)
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    Should be a nice experiment, I'm looking forward to your conclusions.

    I have some Pan Cyan spawn going not myself. It's amazing how fast it expands.

    Good Luck!
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    Old 08-04-08, 21:00   #3 (permalink)
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    AH-HA!

    Now that's what I'm talking about, Spacecake!

    That is what I've been missing...
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    Old 08-05-08, 17:22   #4 (permalink)
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    ...lol

    That's why I posted this tread just a little bit to early...!
    Just for you BB !
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    Old 08-05-08, 17:55   #5 (permalink)
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    THANK YOU !!!!

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    Old 08-06-08, 15:04   #6 (permalink)
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    Here's an update on those developing pins...!
    Harvested them, and dunked again for a couple of hours.
    (picture 1 & 2)

    The colonizing jars,with the clones for this project are half way...
    And I hope they will be ready next Monday or so !
    Looks good so far !
    (pictures 3)





    Attached Thumbnails
    panaeolus-dunk-no-dunk-2nf-flusf-dunk-005int.jpg  panaeolus-dunk-no-dunk-2nf-flusf-dunk-010int.jpg  panaeolus-dunk-no-dunk-clone-brf-grass-int.jpg  
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    Old 08-06-08, 16:36   #7 (permalink)
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    Old 08-06-08, 17:03   #8 (permalink)
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    awesome i was waiting for someone to try this out..

    "archive material"
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    Old 08-06-08, 17:16   #9 (permalink)
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    Yes,I hope it would be a good example guys...!

    I have one question tho...
    I want to try a different casing layer...,I have a pre-mixed bag of Peat/coir and a little compost.
    Looks very good and wanted to add some vermiculiet aswell....
    Shall I try it..?
    Or just use the regular stuff for this time,so I won't screw up ?
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    Old 08-06-08, 17:20   #10 (permalink)
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    yeah why not? can't hurt....

    pans grow invitro with filter patches.

    i don't see why it wouldn't fruit..
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    Old 08-06-08, 17:24   #11 (permalink)
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    Probably will,the ratio's look good too.
    It doesn't hold magnesium,so that a good thing aswell.
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    Old 08-06-08, 17:40   #12 (permalink)
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    Maybe add a little lime (or something else alkaline). Peat can be a little acidic.
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    Old 08-06-08, 17:50   #13 (permalink)
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    I always add cuttlebone to my Pan grows..,works too,but a litlle expensive.
    Most of the time I get trich if I don't use it..especialy after the first flush.
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    Old 08-11-08, 12:11   #14 (permalink)
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    So..today,Monday I prepared the substrate...

    I'm using some horse poo that I found in the woods.
    The poo is a little fresh and had a ph of 4.3
    So.., I took a big pan ,filed it with water.. added two spoons of Calcium and the poo...
    Cooked on low for 30 minutes and measured the Ph,..it was still to high (6.4).
    So I cooked for another 30 minutes without adding more calcium,and now the Ph was 7.4 !

    (it's always very important to give the calcium some time to do his thing !) http://forums.mycotopia.net/fungi-gr...t-calcium.html (Ph adjustment with calcium)

    Drained the water and safed it for some other experiments (picture 1)
    After that I added the same amoud of vermiculiet to the horsepoo,and mixed it up very well (picture 2).
    Stored the mixture in jars and pressure cooked for 70 minutes (picture 3).

    Now it's waiting for the spawn to be ready...
    The jars are almost 100% colonized ,but I don't think the inside is..,so I leave them in the incubater for one or two more days !

    Well,that's it for today...let you all know when the jars are done..
    Attached Thumbnails
    panaeolus-dunk-no-dunk-clone-substrate-1-int.jpg  panaeolus-dunk-no-dunk-clone-substrate-2-int.jpg  panaeolus-dunk-no-dunk-clone-substrate-3-int.jpg  
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    Old 08-11-08, 12:16   #15 (permalink)
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    how do you test you ph?
    i know you can by them cheap meters at lowes you just stick in the dirt. is that accurate enough or do you use something else?

    edit: feel like an ass should've have read the link.
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    Old 08-11-08, 12:27   #16 (permalink)
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    Old 08-11-08, 18:57   #17 (permalink)
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    That poo is nice and fluffy looking!

    Good luck, dude! You know I love your pans...
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    Old 08-11-08, 19:03   #18 (permalink)
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    Thanks Buck...

    Never in my life ,I would have guesed I would take pictures
    of fluffy poo as a hobby....
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    Old 08-11-08, 19:07   #19 (permalink)
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    I'm definitely pulling up a seat and anxious to see the results. I think experimenting is the best way to learn.. You got my interest. Good Luck Spacecake. imo we need more threads like this.
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    Old 08-11-08, 19:16   #20 (permalink)
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    Quote:
    Originally Posted by spacecake View Post
    Thanks Buck...

    Never in my life ,I would have guesed I would take pictures
    of fluffy poo as a hobby....
    And get compliments on your poo!
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    Old 08-12-08, 13:24   #21 (permalink)
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    Okay..,little update...

    The jars are fully colonized (picture 1)
    (the uncolonized stuff you see at the bottom is just plain verm.)

    So,..I crumbled up every cake in seperate plastic bags...
    Added two jars of casing material to each cake/bag and mixed it up very good !
    I have know three bags for three trays ready... (picture 2)

    Finally I filled the tray up and they are now stored in the incubator for two or three days. (picture3)

    In the meanwhile,I made a mixture for the casing layer,with 50% Vermiculiet,and 50% of pre-mixed materials containing Peat/Coir and compost.
    It felt very loose after mixing it all up,and I think it will work very well..atleast I hope so ! (last picture)
    It's also been sterilized ,and I will add it as soon as the trays are fully colonized.

    I'm very excited at the moment and I hope it will turn out well !
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    panaeolus-dunk-no-dunk-three-fully-colonized-jars-int.jpg  panaeolus-dunk-no-dunk-casing-mixture-bags-int.jpg  panaeolus-dunk-no-dunk-three-casing-traysint.jpg  panaeolus-dunk-no-dunk-casing-top-layer-substrateint.jpg  
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    Old 08-12-08, 17:54   #22 (permalink)
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    Neat thread Space..!
    Your really gett'n good with those Pans mang..
    What's your FC setup like..
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    Old 08-12-08, 18:01   #23 (permalink)
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    Thanks,Golly...!
    Last year I made a new terrarium..,maybe you remember...
    http://forums.mycotopia.net/grow-cha...terrarium.html (New terrarium)
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    Old 08-12-08, 23:46   #24 (permalink)
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    beautiful pans space
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    Old 08-13-08, 09:20   #25 (permalink)
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    One day later...,all three casings are already 100% recovered !

    So I have aplied the top casing layer (0.5 cm thick) and returned them to the incubator...
    For who wants to know,the incubator temperature is 27 oC.
    ..Sorry for the blurry picture,but I was very afraid of contams,if I took to much time to take a good picture...

    Going great so far...!

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    Old 08-15-08, 14:04   #26 (permalink)
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    Here's a little update...

    I took the trays out of the incubator and placed them in the terrarium.
    They will get indirect natural light and fresh air for another two hours,..and after that I'm gonne take the first tray out,and dunk it for aboud two to three hours...
    I will not dunk the tray at this time,because I want to give every tray the same amound of light,and it will be dark soon.

    I have to be carefull dunking,because the mycelium is not as strong as after a first flush,it will break apart easily...
    Maybe I should have wait a little more longer before taking them out of the incubator,but I don't have alot of time (leaving tommorow).
    Here are some pictures...

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    panaeolus-dunk-no-dunk-1st-day-clone-terrarium-002int.jpg  panaeolus-dunk-no-dunk-1st-day-clone-terrarium-004int.jpg  panaeolus-dunk-no-dunk-1st-day-clone-terrarium-006int.jpg  
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    Old 08-15-08, 15:15   #27 (permalink)
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    excellent!
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    Old 08-15-08, 19:43   #28 (permalink)
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    Oeps almost forgot the dunk...!
    Was tripping heavy..lol

    I dunked it for a total of 4 hours...
    I weighted the undunked tray and the dunked one.

    The dunked tray had gained 100 grams of weight !
    It's still looks very solid and good !

    exciting....
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    Old 08-15-08, 20:43   #29 (permalink)
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    Totally cool, man!

    I am anxious to see what the dunked tray will do.

    Do you incubate your trays with the plastic wrap on them tight?
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    Old 08-15-08, 21:46   #30 (permalink)
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    Yep I do....
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    Old 08-23-08, 13:16   #31 (permalink)