Transkei on agar

pihop · 04-25-09, 09:05

Well I just transferred some transkei myc from two contammed dishes onto 8 new ones with H2O2.
I use quite a high conc of H2O2 this time to hopefully prevent most contams.
I used 1:20 17.5% H2O2 wich gives a conc. of ~0.8% H2O2 agar.

Let's pray that the dishes wont contam!

(I havn't used a glovebox or anything for the open air work)

Lazlo · 04-25-09, 12:23

There's a ton of bacteria brewing in those plates. BUT, there is also a lot of mushroom mycelium as well. Take a sewing needle or a scalpel (sterilized) and use it to shave a tiny piece of mycelium off of the top of a nice spot. Very little and transfer that to a new plate or 5. Use sanitized gloves and a glove box. That open air stuff is weak at best. Good luck.

pihop · 05-06-09, 13:28

None of the dishes has showm any sign of growth yet (no contams though). I probably used WAY to much H2O2.

Today I transferred a contammed dish to 7 salt agar dishes (good TEK lazlo

). Since my only contams this far are bacterial the salt will keep them off.

pihop · 05-09-09, 09:55

It seems the recovery time on agar is fairly long

I also transferred some myc to wet cardboard and that is already fluffing up nicely and taking over the cardboard.

pihop · 05-10-09, 10:17

Four dishes are starting to fluff up and recover.
No contams yet.
Will return with pics once you can see the little buggers growing.

mushhut · 05-10-09, 14:48

Were the 1st dishes you did from ms or did you use live tissue? Hope your transfers work out for you.

pihop · 05-10-09, 16:50

First dishes were MS.

pihop · 05-25-09, 17:00

The dishes have been growing at very varying rate. Two have almost taken over the agar whilst (proper term to use instead of while?) the rest are slacking behind. The ones that have colonized quickly also grows quite uneven.

spacecake · 05-25-09, 17:43

What are your next plans with those Transkei dishes ?

pihop · 05-25-09, 17:55

To nocc up some popcorn jars and transfer the best ones to new dishes.

spacecake · 05-25-09, 17:57

Okay,I'm just a big fan of this strain..so I'm very excited...

pihop · 05-25-09, 17:58

Quote:

Originally Posted by spacecake

Okay,I'm just a big fan of this strain..so I'm very excited...

Then I will have to do my best then :P
I can feel the pressure now that I have one of the best growers in here watching my thread

spacecake · 05-25-09, 18:05

....

I'm working an some Trankei too...
Yellow and red ones.
The yellow (original form Afrika..I think I got it from phlux) contaminated on me...
I will take a clone of the red ones (from a cake) when it's finished it's flush

Maybe I shall ask him again for another print,because I want to grow them side by side.

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04-25-09, 09:05	#1 (permalink)
pihop Necrophiliac Join Date: Jan 2009 Posts: 294	Transkei on agar Well I just transferred some transkei myc from two contammed dishes onto 8 new ones with H2O2. I use quite a high conc of H2O2 this time to hopefully prevent most contams. I used 1:20 17.5% H2O2 wich gives a conc. of ~0.8% H2O2 agar. Let's pray that the dishes wont contam! (I havn't used a glovebox or anything for the open air work) Attached Thumbnails __________________ Current grow: Orissa India

04-25-09, 12:23	#2 (permalink)
Lazlo old hand Join Date: Mar 1970 Posts: 7,591	There's a ton of bacteria brewing in those plates. BUT, there is also a lot of mushroom mycelium as well. Take a sewing needle or a scalpel (sterilized) and use it to shave a tiny piece of mycelium off of the top of a nice spot. Very little and transfer that to a new plate or 5. Use sanitized gloves and a glove box. That open air stuff is weak at best. Good luck. __________________ How can you have any pudding, if you don't eat your MEAT?

05-06-09, 13:28	#3 (permalink)
pihop Necrophiliac Join Date: Jan 2009 Posts: 294	None of the dishes has showm any sign of growth yet (no contams though). I probably used WAY to much H2O2. Today I transferred a contammed dish to 7 salt agar dishes (good TEK lazlo). Since my only contams this far are bacterial the salt will keep them off. __________________ Current grow: Orissa India

05-09-09, 09:55	#4 (permalink)
pihop Necrophiliac Join Date: Jan 2009 Posts: 294	It seems the recovery time on agar is fairly long I also transferred some myc to wet cardboard and that is already fluffing up nicely and taking over the cardboard. __________________ Current grow: Orissa India

05-10-09, 10:17	#5 (permalink)
pihop Necrophiliac Join Date: Jan 2009 Posts: 294	Four dishes are starting to fluff up and recover. No contams yet. Will return with pics once you can see the little buggers growing. __________________ Current grow: Orissa India

05-10-09, 14:48	#6 (permalink)
mushhut Mycotopiate Join Date: May 2008 Posts: 875	Were the 1st dishes you did from ms or did you use live tissue? Hope your transfers work out for you.

05-10-09, 16:50	#7 (permalink)
pihop Necrophiliac Join Date: Jan 2009 Posts: 294	First dishes were MS. __________________ Current grow: Orissa India

05-25-09, 17:00	#8 (permalink)
pihop Necrophiliac Join Date: Jan 2009 Posts: 294	The dishes have been growing at very varying rate. Two have almost taken over the agar whilst (proper term to use instead of while?) the rest are slacking behind. The ones that have colonized quickly also grows quite uneven. Attached Thumbnails __________________ Current grow: Orissa India

05-25-09, 17:43	#9 (permalink)
spacecake Universal Mod Join Date: May 1972 Posts: 4,734	What are your next plans with those Transkei dishes ? __________________ "As a child, i could walk on the ceiling. I'd butterfly up on the walls"

05-25-09, 17:55	#10 (permalink)
pihop Necrophiliac Join Date: Jan 2009 Posts: 294	To nocc up some popcorn jars and transfer the best ones to new dishes. __________________ Current grow: Orissa India

05-25-09, 17:57	#11 (permalink)
spacecake Universal Mod Join Date: May 1972 Posts: 4,734	Okay,I'm just a big fan of this strain..so I'm very excited... __________________ "As a child, i could walk on the ceiling. I'd butterfly up on the walls"

05-25-09, 18:05	#13 (permalink)
spacecake Universal Mod Join Date: May 1972 Posts: 4,734	.... I'm working an some Trankei too... Yellow and red ones. The yellow (original form Afrika..I think I got it from phlux) contaminated on me... I will take a clone of the red ones (from a cake) when it's finished it's flush Maybe I shall ask him again for another print,because I want to grow them side by side. __________________ "As a child, i could walk on the ceiling. I'd butterfly up on the walls"

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