My 1st Iso

[mushhut]

This is my first Iso. Strain is god seekers. Last time I grew this strain the fruits were all 1/4inch long and never got any bigger on all 4flushes. They were very potiant. I was upset with the size of the fruits so I decided to isolate the strain. I did one drop of spore solution to agar. From there I transfered the most rizo to another dish then again transfered to 2 more plates. I cut out a wedge from the final plate and droped into a rye jar. I took that one jar and did a small hpoo and straw tuperware container. I just tipped the shit brick out of the tuperware and dec it like a cake. When I took it out of the container I noticed there were 3pins on top. I hope I didnt mess up the pins. Heres a pic of the bottom of the shit brick.

If this lil brick spits off a good yeild I willl be making a master slant and a large mono tub with this isolate.

[junior1]

Best of luck to ya, Gonna be cool to see how your ISO plays out.

[mushhut]

Things are looking good for the iso grow so far. Pins are looking much differant then the last grow. Pics to come!

[GadgetGuy]

Where did you get god seekers? Do they always grow small like you were saying? I like the sound of "very potent."

[eatyualive]

Originally Posted by mushhut

This is my first Iso. Strain is god seekers. Last time I grew this strain the fruits were all 1/4inch long and never got any bigger on all 4flushes. They were very potiant. I was upset with the size of the fruits so I decided to isolate the strain. I did one drop of spore solution to agar. From there I transfered the most rizo to another dish then again transfered to 2 more plates. I cut out a wedge from the final plate and droped into a rye jar. I took that one jar and did a small hpoo and straw tuperware container. I just tipped the shit brick out of the tuperware and dec it like a cake. When I took it out of the container I noticed there were 3pins on top. I hope I didnt mess up the pins. Heres a pic of the bottom of the shit brick.

If this lil brick spits off a good yeild I willl be making a master slant and a large mono tub with this isolate.

were they fat and stubby? im thinking koh samui. try cloning a good fruit. then isolating. looks good!

[Frequency]

Very rhizomorphic brick you've got there Mush! Be sure to keep us posted on the grow! I've had some real shorties that have knocked my socks off before. Let's hope you get a forest!

[mushhut]

Cant say where I got the spores from. I can say that I got the spores before I found this site. Eats the shrooms were just short maybe only 1/4 inch tall never matured. Im not totaly satisfied with this flush but it looks a lot beter then the first time I grew these from ms. I am going to clone that cluster you see in the pics. I am leaving for a trip 2mow and wont be back till next week. Im going to leave these in the tub and hope they dont get over growen while Im gone.

[stimpy]

Looks agressive as hell

[mushhut]

Didnt get to clone the cluster do to leaving outa town. I had to harves all the fruits before I left. Here are the pics from last weeks harvest. There are pins coming around for round 2. I have no idea why there is a little flare in the caps. Im not very impressed but its a lot beter then the ms grow I had with this strain.

[feralronin]

Awesome to see a first cloning attempt go well for ya.

Its def one of the harder parts of this hobby IMO.

Way to go.

[stimpy]

was the original clone from a cluster?

[mushhut]

This wasnt a clone at all. I shot 1 drop of ms onto an agar plate and did several transfers to other agar plates untill I got a good looking plate. I took that agar plate and cut a wedge and tossed it to a rye gran jar. After the grain jar was colonized I spawned to hpoo and straw. Here is the giant I got from this small tub. I did clone this fruit to 4 rye jars, but oonly one turned out. Im going to do g2g today with my 1/2 pint of cloned rye. This shroom was over 110g fresh!!! I will post results of my first clone in this thread.

[designlogic]

Good work! That shroom is a monster
Good Luck with the cloning project.

[nra9785]

Indeed, Best of luck. Ill be watching this. Very intriguing

[Brakiska]

nice results
proves how worth this is

whats your workdplace?
flowhood, glovebox?

thankx for sharing

[RestartLater]

That is one big boomer :+}~~

I got a 1 oz. Texas the other day and felt good about it Mush.......LOL But damn man!! WTG !!

RestartLater

[mushhut]

Originally Posted by Brakiska

nice results
proves how worth this is

whats your workdplace?
flowhood, glovebox?

thankx for sharing

I poured all my plates and did all my transfers in a home made glove box. The box isn't anything special but works. I took the clone in a hurry. I did my cloning in open air. I split the shroom and took clean tweesers and pulled a peice from the inside and droped the peices in 5 different jars. I did my cloning in open air because of time constants, I'm very happey 1 jar made it. I do almost everything open air. I do not have a flow hood. If I do agar work or transfers I resort to a pos home made glove box.

[pihop]

Holy freaking hell those are some big shrooms!

[Sidestreet Getaway]

Nice fistful!

[mushhut]

Sorry I havent been updating. One of the 5 jars made it without contam. I transfered 1/2 of that jar to 2 quart jars and got green. After that I took 2 peices of grain from the other 1/2 of the good jar and transfered to 2 dishes. I got good growth and made a 3rd transfer to 5 more dishes 3 of 5 made it. Now Im at a stand-still (got too much going on). Heres some pics. I cant wait till I have time to play with this!

First pic is of the 1st dish transfer. You can see where I cut out the mycelium. the next are of the new transfers. The last pic is of a dish that has contam.

[P4ulada]

Nice plates! You´re always have big fruits

Congratz! Anxiety to see the fruits from the isolated myc!

[Indonesia]

your isolate looks very aggressive and rhizomorphic. looking awesome in the first picture of the brick. thats what foaf likes to see! was the monster mushroom the first to pin?

[copelandiaKidd]

Beautiful mushies!!!!! potency along the same lines as last time, or no taste yett?

[mushhut]

Originally Posted by Indonesia

your isolate looks very aggressive and rhizomorphic. looking awesome in the first picture of the brick. thats what foaf likes to see! was the monster mushroom the first to pin?

This is no longer an iso but a clone. This has been a project that is a year old now. I started with 1 drop of ms to 3 petri dishes made several transfers. fruited the best looking dish. Then cloned the 110g boomer to rye berries. Transfered rye to 2 dishes, grew them out then transfered to 5 more dishes. So the mycelium you are seeing in my last pics are of a 110g clone. I'm wanting to make a master slant so I can keep this clone forever.
All in all its taken me 1yr to get to this point. I could have worked faster but I'm a very busy person. Ill keep updating as things progress.

[mushhut]

Originally Posted by copelandiaKidd

Beautiful mushies!!!!! potency along the same lines as last time, or no taste yett?

Yep potency is deffentaly somthing to write home about. Very great, light body load and visuals that will blow your mind. We will see how the clone does in the next few months.

[waterchilde]

I've been reading, looking for the answer to a question that I have and I think you just did that. I want to know if I can take one colonized grain of rye and culture it on a petri dish to isolate the rizo, makes sense to me but I am fairly new at this and just want to know for sure... Thanx...

[sandman]

sure, you can do that waterchilde. Just gotta figure out how to get a piece of colonized grain onto the plate as sterile as possible...perhaps sterilized forceps or something.