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Old 09-21-09, 12:18   #1 (permalink)
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Talking Hillbilly bulk sub photo essay - spawn through harvest LOTS OF IMAGES

I wanted to photographically document what a bulk substrate looks like from spawning through harvest. I encourage anybody with a decent camera to do the same. You will learn a lot looking at high res pictures of the developing mycelia. It is also lots of fun to play the “guess where the pins will form” game…

Strain: Hillbilly
Inoculant: Spore Syringe
Spawn: Popcorn
Spawn Ratio: 1:3 of popcorn:bulk
Bulk Substrate: 40:40:20 / coir:castings:Poly (by volume)
Substrate depth: 4in
Top Dressing: thin layer of substrate to cover exposed popcorn
Spawn Run Temp: 83F-84F
Pinning Temp: 83F-84F
Relative Humidity: 80%-90% (continuously)
Pins noted 11 days from spawning

Spawn Run Time Line:
Day 0 – Popcorn spawned to bulk substrate
Day 1 – First picture after 24hrs incubation
Day 6 - Fruiting Conditions initiated
Day 11 – Pins noted, macro shots taken
Day 12 – Good pin set noted, macro shots taken
Day 14 - Harvest

Time Line:
Day 1 – 2 pint popcorn jar inoculated with .5mL spore water
Day 5 – Popcorn jar 70% - violent shake
Day 9 – Popcorn jar 100% - violent shake
Day 10 – Popcorn jar converted to slurry, popcorn spawn bag inoculated with slurry
Day 14- Popcorn bag kneaded
Day 18- Popcorn bag kneaded
Day 19 – Popcorn spawned to bulk substrate
Day 20 – First picture
Day 24 – Fruiting Conditions initiated
Day 29 – Pins noted, macro shots taken
Day 30 – Good pin set noted, macro shots taken
Day 33 - Harvest


Process:
A pint canning jar with silicone injection ports and a double Tyvek vent was filled 2/3 with hydrated popcorn. A medium pre-sealable spawn bag from sporeworks.com was filled with 10 cups of hydrated popcorn and 1 cup of bulk substrate and sealed with an Impulse sealer. Substrate and popcorn were mixed thoroughly before filling the bag. The spawn bag and pint jar were sterilized at 15psi for 90 minutes

After cooling completely, the pint jar was inoculated with .5mL of Hillbilly spore water, shaken and incubated at 84F-85F. When approximately 70% colonized, the popcorn jar was shaken thoroughly. At 100% colonization the popcorn jar was shaken thoroughly. After the jar was again 100% colonized, it was shaken violently to prepare for mycelia extraction.

180mL of sterile water was injected into the popcorn pint jar. The jar was shaken violently for 5 minutes to break up the mycelia and distribute it into the water. Using a 60mL syringe, as much water was removed from the pint jar as possible. Approximately 165mL of mycelia slurry was recovered from the pint jar.

All mycelia slurry was used to inoculate the spawn bag through a silicone injection port. The slurry was distributed across as much of the popcorn as possible and the bag was kneaded thoroughly after inoculation. The bag was then incubated at 84F-85F until colonized 50%. The bag was then kneaded and incubated until colonized 100%.

30 cups of bulk substrate (40:40:20 coir:castings:Poly - by volume) was prepared. All substrate components were hydrated to field capacity and then mixed. The bulk substrate was loaded into 3 medium sporeworks.com pre-sealable spawn bags, sealed and sterilized at 15psi for 90 minutes.

The 100% colonized popcorn spawn bag was thoroughly kneaded to break up all mycelia chunks. After kneading the spawn bag was incubated at 84F-85F for 24 hours to allow it time to recover.

Popcorn spawn and bulk substrate were mixed in a large plastic tub. Approximately 10% of the bulk substrate was held back and used as a top layer on the tub, covering all exposed popcorn. The tub was very loosely covered with aluminum foil and incubated at 82F-83F until 80% colonized.

At 80% colonization, the aluminum foil was removed and the tub was placed in terrarium with a 12/12 florescent light source and 80%-90% RH. The terrarium was provided approximately 6 FAE per day via an aquarium air pump.

Pictures of the substrate were taken every day at approximately the same time, from 24 hours after initial spawning until pins were observed and well developed.



Notes:
The popcorn pint was shaken an excessive number of times and allowed to recover to test for bacterial contamination. Any contamination in the popcorn pint would have doomed the entire project to failure. If the jar had failed to recover quickly from any of the shakes, it would have been discarded immediately.

The idea to inject water into popcorn jars, shake and the remove mycelia slurry comes from Fahtster. I thank him from the bottom of my heart for introducing me to the idea. I find this Tek to be infinitely more valuable than liquid culture for producing large volumes of guaranteed sterile inoculant. Thank you Faht, wherever you are!!!

The “poly” in my bulk substrate is polyacrylamide. Also known as water crystals. I do NOT want to get into an argument about poly being toxic, etc. If poly does not appeal to you, vermiculte can be used as a replacement.

Incubating bulk substrates at 82F-83F is a risky proposition. If your environment is dirty or your techniques are poor, the chances of loosing a bulk sub to contamination are dramatically increased above 80F. A large bulk substrate incubated at 83F ambient will easily reach 86F-87F internally. Metabolites will precipitate on the substrate surface and they need to be removed via syringe or absorbent material. Excessive metabolite buildup will inhibit pinning.

I do not recommend that YOU incubate in a warm/wet 83F environment until you have all your environment variables dialed in solid. Seriously – contaminants grow very, very quickly at 83F.

But…I do not practice what I preach because I’m not the type of person I’m preaching to…
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Last edited by BuckarooBanzai; 09-23-09 at 16:28.
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Old 09-21-09, 12:34   #2 (permalink)
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Day 1. 24 hours of incubation. Mycelia just peeking through in a couple spots.


Day 2. 48 hours of incubation.


Day 3. 72 hours of incubation.


Day 4. 96 hours of incubation.



Day 5. Growth is excellent. Some metabolites (myc piss - the yellow liquid) is removed with the corner of a paper towel.



Day 6. Growth is proceeding rapidly. Pinning conditions introduced today.


Day 7.


Day 8.
Attached Thumbnails
hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-1day-24hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-2day-48hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-3day-72hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-4day-96hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-5day-120hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-6day-144hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-7day-168hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-8day-192hours.jpg  
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Old 09-21-09, 12:42   #3 (permalink)
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Day 9 - knots that will become pins are visible! Can you find them?


Day 10 - Many more knots and even a few primordia!


Day 10- Upper left quadrant macro - definite primordia and pins.


Day 10 - Lower left quadrant macro - definite primordia and pins




Day 10 - Upper right quadrant macro



Day 10 - Lower right quadrant macro
Attached Thumbnails
hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-9day-216hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-10day-240hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-10days-upperleft.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-10days-lowerleft.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-10days-upperright.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-10days-lowerright.jpg  
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Old 09-21-09, 12:48   #4 (permalink)
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Okay, here we are at day 11. Pins a-plenty at this point. I think this tray should have incubated an additional 24 hours before fruiting conditions were initiated...

Day 11 - whole tray



Day 11 - Center quadrant macro


Day 12 - Upper left quadrant macro



Day 13 - Lower left quadrant macro


Day 13 - Upper right quadrant macro


Day 13 - Lower right quadrant macro
Attached Thumbnails
hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-11days-264hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-11days-center.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-11days-upperleft.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-11days-lowerleft.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-11days-upperright.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-11days-lowerright.jpg  
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Old 09-21-09, 12:54   #5 (permalink)
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Day 12, whole tub:


Day 12 upper left quadrant:


Day 12 lower left quadrant:


Day 12 upper right quadrant. Please disregard the crushed primordia. Somebody idiot the light on 'em while taking pictures...


Day 12 lower right quadrant:
Attached Thumbnails
hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-12days-288hours.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-12days-upperleft.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-12days-lowerleft.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-12days-upperright.jpg   hillbilly-bulk-sub-photo-essay-spawn-through-harvest-lots-images-12days-lowerright.jpg  
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Old 09-21-09, 12:55   #6 (permalink)
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So that's my fun little game. How many pins could you find developing after seeing what the same area looked like 24 hours later?

Seeing detail in pictures is sometimes easier than seeing it with your eyes...

I have much higher resolution shots I could upload, but I think there is enough detail visible in those.

You thoughts, Hobson?
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Old 09-21-09, 13:29   #7 (permalink)
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Nice job. I love when somebody has daily updates. It's almost as if I'm growing them myself (without the happy ending, anyway.)
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Old 09-21-09, 15:24   #8 (permalink)
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Quote:
Bulk substrate : 40:40:20 / coir:castings:Poly (by volume)
What is "Poly"?

Poly-what? not poly-fill, right?

Polywogs?
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Old 09-21-09, 15:28   #9 (permalink)
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Polyacrylamide. Water crystals. The binder in straw net. Dangerous and deadly toxins...

Verm does almost as well in the role of water reservoir for the sub.
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Old 09-21-09, 15:38   #10 (permalink)
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For people who want to read more about poly crystals there's very good info and pictures in this thread:

http://forums.mycotopia.net/misc-mag...tml#post292135 (BB Tek - A complete walk through; spores to LC to jars to bulk substrate)

Scoot down to this post to see pictures of the crystals hydrating:

http://forums.mycotopia.net/misc-mag...tml#post292135 (BB Tek - A complete walk through; spores to LC to jars to bulk substrate)
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Old 09-21-09, 16:01   #11 (permalink)
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Awesome thread BB! I'd rep ya but I gotta share the love some more.

In the next couple weeks with your updates this will be even more amazing
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Old 09-21-09, 17:22   #12 (permalink)
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damn those are some plump pins!
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Old 09-21-09, 21:06   #13 (permalink)
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Well cletus i tell ya what them ol boys from east tx keep stealin mah chickens gonna get one of them, whatchama call russian rifles keep em off my lawn light em up from yonder good 150 yards
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Old 09-21-09, 22:03   #14 (permalink)
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I lovvveee ittt!!!#@$##%~

BB u rock! <3 <3 <3 I've been hearing some great things about Hillbilly and this thread certainly illuminates it's potential.
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Old 09-21-09, 22:44   #15 (permalink)
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Nice job there BB , Hope my Hillbilly's turn out like that. Just spawned mine to bulk a few days ago.
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Old 09-21-09, 22:47   #16 (permalink)
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Nice work BB. Beautiful documentation!
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Old 09-22-09, 12:17   #17 (permalink)
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Day 13 since spawning:





Even from multispore, the Hillbilly strain is a giver.
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Old 09-22-09, 13:47   #18 (permalink)
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looking good...I hope my hillbillys turn out that dense of a pin set...another week or so and ill kno..yay...great photos btw
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Old 09-22-09, 14:10   #19 (permalink)
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From my previous experience, this is a very good pin set for Hillbilly from multispore.

I do already see the characteristic size difference in fruits. HB does not like to throw a level canopy of similarly sized 'shrooms!
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Old 09-22-09, 18:38   #20 (permalink)
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Looking good Buck!

I know what you're saying about the even canopy but with that pin set....your
gonna have a canopy never the less
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Old 09-22-09, 23:27   #21 (permalink)
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Beautiful pics, excellent write up.
I see a lot of effort went into documenting this grow.
Thanks for sharing.
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Old 09-23-09, 00:45   #22 (permalink)
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Wouldnt mind giving this a shot myself, thanks for the inspiration mang! respect! and awesome documentation!
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Old 09-23-09, 13:34   #23 (permalink)
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Man thats a good set up and its seems to work great for that type of growing.
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Old 09-23-09, 15:53   #24 (permalink)
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Day 14 - harvest:



I'm happy.
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Old 09-23-09, 17:37   #25 (permalink)
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That is a full tub of HBs--very beautiful. Thanks for posting.
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Old 09-23-09, 18:34   #26 (permalink)
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Damn right! and Im happy for ya,


Dunk and round two?
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Old 09-23-09, 18:57   #27 (permalink)
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It almost looks like there is a whole new pinset coming in as those are ready to be harvested. Sweet tub man!
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Old 09-23-09, 20:31   #28 (permalink)
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I don't actually dunk. One of the advantages of that old devil polyacrylamide...

I flood the surface to rinse it and then dump the excess moisture out of the tub. Then back into the FC for round 2. Then, onto the compost heap this sub shall go...

Gadget - all those pins you see are aborts. None will mature. Virtually all of them were removed when the tub was harvested.
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Old 09-25-09, 17:50   #29 (permalink)
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Yeah, I do similar with trays. Except I let em soak for 8-15 hours depending on how pores/tight they are colonized if ya know what Im saying.
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Old 09-25-09, 18:12   #30 (permalink)
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Very nice! I'm looking forward to seeing a second flush from that tray!
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Old 09-25-09, 20:43   #31 (permalink)
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Awesome harvest and photos

Do, I see vert on the fruit ?
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Old 09-25-09, 20:59   #32 (permalink)
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Oh yeah, beaucoup vert. Almost unavoidable when you fruit so warm without a sterile fruiting environment. You will notice it worst on the oldest/largest fruits - they have had time to really "steep" in it.

Bacteria love warm and moist conditions. Fortunately, verticillium fights off other bacterial contams without drastically affecting harvest weight...
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Old 09-26-09, 12:47   #33 (permalink)
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Do you always put your subs. into fruiting conditions when they aren't 100% colonized like that? I tried this with my latest two tubs and I'm gonna see if it helps the pinset or overall yield in the end.
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Old 09-26-09, 14:19   #34 (permalink)
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Quote:
Originally Posted by BuckarooBanzai View Post
Incubating bulk substrates at 82F-83F is a risky proposition.

So why risk it, what's the rush?
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Old 09-28-09, 22:28   #35 (permalink)
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Quote:
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Do you always put your subs. into fruiting conditions when they aren't 100% colonized like that? I tried this with my latest two tubs and I'm gonna see if it helps the pinset or overall yield in the end.
I don't recommend it. Better to wait until a sub is 100% colonized before fruiting.


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So why risk it, what's the rush?
This was an experiment...I like experiments...
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Old 10-02-09, 23:10   #36 (permalink)
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Firstly, yeah - I let these go too long and they splooged spores all over everything. Second - I'm not at all sure why they sprouted so much mycelia on the cap this time. Probably 'cause they went so long before being harvested!

Second flush:




I think I will let this casing go for a third flush in the terrarium environment. I'm interested to see what it will do.

I was quite surprised by the size of the second flush. It actually weighed more than the first flush. I haven't seen performance this good from some of my isolates (this was a multispore project). I think Teo smiled at this grow.

This is a good example of why you should ALWAYS keep a refrigerated grain master of any project. You never know how well a strain will perform until after a grow is finished.

And the bio-assay division of The Banzai Institute for Higher Education (The Banzai Institute for Higher Education: BuckarooBanzai's Tek Index) reported they were also very, very happy with this project. The come up is incredibly smooth with remarkably little of the "nervous energy" generally associated with mushrooms.

I friggin' love the Hillbilly strain.
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Old 10-03-09, 06:06   #37 (permalink)
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Those shrooms are massive from your second flush. HBs are an underrrated strain, to be sure.
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Old 10-12-09, 12:37   #38 (permalink)
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3rd flush:



Should have harvested this about a day before, but was busy doing other things. Very surprised by the size of this flush.

Good stuff, though. Definitively demonstrated to me that cubensis growth speeds are strongly affected by ambient temps.
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Old 10-12-09, 12:41   #39 (permalink)
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No complaints there.

Spores seem to be light for such mature boomers.
Thats a good thing.
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Old 10-12-09, 13:37   #40 (permalink)
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Very nice. Gotta love the lack of nervous energy, and I love the cup shape of those mature caps. Go BB!
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Old 10-14-09, 09:07   #41 (permalink)
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good read. Very nice work bb.

Like how ya shake master jars many times to make sure they are good to use..

Fine work indeed
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Old 10-14-09, 11:03   #42 (permalink)
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Great thread! Very interesting, good pics too. Looks like something SWIM might be into. Nice work man
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