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| | #1 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | Hillbilly bulk grow in a 25 gallon aquarium
Strain: Hillbilly Inoculant: Mycelia slurry Spawn: Popcorn Spawn container: Medium pre-sealable filter patch spawn bags from sporeworks.com Spawn Ratio: 1:3 / popcorn:bulk Bulk Substrate: 40:40:20 / coir:castings:Poly (by volume) Substrate depth: 4in Spawn Run Temp: 83F-84F Pinning/cropping Temp: 75F-80F Process: A pint canning jar with silicone injection ports and a double Tyvek vent was filled 2/3 with hydrated popcorn. Three medium pre-sealable spawn bags from sporeworks.com were filled with 10 cups of hydrated popcorn and 1 cup of bulk substrate and sealed with an Impulse sealer. Substrate and popcorn were mixed thoroughly before filling the bags. The spawn bags and pint jar were sterilized at 15psi for 90 minutes After cooling completely, the pint jar was inoculated with .5mL of Hillbilly slurry, shaken and incubated at 84F-85F. When approximately 70% colonized, the popcorn jar was shaken thoroughly. At 100% colonization the popcorn jar was shaken thoroughly. After the jar was again 100% colonized, it was shaken violently to prepare for mycelia extraction. 180mL of sterile water was injected into the popcorn pint jar. The jar was shaken violently for about 3 minutes to break up the mycelia and distribute it into the water. Using a 60mL syringe, as much water as possible was removed from the pint jar. Approximately 165mL of mycelia slurry was recovered from the pint jar. This process was repeated 3 times to produce approximately 490mL of slurry inoculant. Each spawn bag was injected with about 160mL of slurry through a silicone injection port. The slurry was distributed across as much of the popcorn as possible and the bags were kneaded thoroughly after inoculation. The bags were then incubated at 84F-85F until colonized 50%. The bags were then kneaded and incubated until colonized 100%. 90 cups of bulk substrate (40:40:20 coir:castings:Poly - by volume) was prepared. All substrate components were hydrated to field capacity before mixing. The bulk substrate was loaded into 9 medium sporeworks.com pre-sealable spawn bags, Impulse sealed and sterilized at 15psi for 90 minutes. The 100% colonized popcorn spawn bags were thoroughly kneaded to break up all mycelia chunks. After kneading the bags were incubated at 84F-85F for 24 hours to allow time for recovery and test for bacterial contamination. Popcorn spawn and bulk substrate were mixed in the aquarium with a large spoon. Approximately 10% of the bulk substrate was held back and used as a top dressing, covering all exposed popcorn. The Aquarium was covered with aluminum foil (on top) and incubated at 83F-84F until the substrate was completely colonized. The foil “lid” had 8 holes in it, each about the size of a quarter. No attempt was made to tape the foil down, it was just press fitted to allow some fresh air exchange. The same aluminum foil “lid” was used for fruiting.. Fruiting was initiated by putting the aquarium in diffuse natural light (no direct sunlight). Fresh air was provided by manual fanning 3 times per day. No automated air exchange was used. No misting was required. Time frame was 31 days from inoculation to harvest of the first flush. Pictures of the aquarium (top and side) before harvest: ![]() ![]() I love the Hillbilly strain! Such a giver. Bulk substrates are fun...
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #4 (permalink) |
| howling mad Join Date: Jan 2007
Posts: 4,044
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Cool, I see mycelium consuming some shrooms. I see that with pans mostly. Can't say that any of my cubes have ever done it. Although I chop mine down a bit sooner than some.
__________________ WOODY: Hey, Mr. Peterson, there's a cold one waiting for you. NORM: I know. If she calls, I'm not here.[ (Cheers) |
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| | #5 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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Overly humid conditions cause that "reversion" growth where the caps turn back into growing myc. Mr. Aquarium should have been fanned a couple more times each day. Maybe slightly larger breather holes as well.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #8 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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Nope, everything is in the aquarium. Check the pic. The substrate represents a little less than 1/3 of the aquarium's total volume (about 6-7 gallons of substrate). 6 gallons is 96 cups. We are so stupid for not having standardized on the metric system. Looking that conversion up on Google was annoying. But how the hell ya gonna remember that a gallon is 16 cups? What kind of goof ball uses base 16 counting? Hexadecimal is for computers and gate based logic. Not people. So friggin' stupid.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #9 (permalink) |
| Mycophiliac Join Date: Jul 2009
Posts: 159
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BB, I aspire to growing a bulk like that... ![]() So, you sterilize your sub instead of pasteurizing? Also, When you say the foil lid was used in fruiting, do you mean you put the foil over the top of the aquarium so light would only come in from the sides? |
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| | #10 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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That bulk is worm castings and coconut coir and I sterilize it at 15psi for 90 minutes. You would not want to sterilize manure or straw, but it can very safely be done with castings and coir. The only light was ambient/indirect natural light coming in the sides of the aquarium. The whole top was covered with aluminum foil.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #13 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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The spawn bags take about 5-6 days to fully colonize. A full cup of coir:castings:Poly (the bulk sub) is mixed into each popcorn bag before it is inoculated. That soaks up all the extra water and gets the myc to start producing the enzymes it will need to digest the bulk sub. You should be able to sterilize coir:verm with no problem at all.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) Last edited by BuckarooBanzai; 10-04-09 at 09:59. Reason: subject/verb agreement |
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| | #19 (permalink) |
| Mycophiliac Join Date: Oct 2009
Posts: 10
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Wow. That's one of the most impressive bulk grows I ever seen. That's very big mushrooms you have there and a beautiful canopy. As mentioned, very inspirational. Thanks for sharing the pictures and info. ![]() Why do you shake the popcorn jar one more time after it's 100%? |
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| | #21 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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Why thank ya'll kindly. I am just applying the lessons I learned here at Mycotopia University. Any one of you could do this exact same project at home. Popcorn and bulk substrate are easy to prepare (or order from Mycrotopia). The popcorn jar is turned completely into mycleia slurry by flooding it with sterile water and shaking it violently (multiple times). Mycelia grows so fast on popcorn that it can overtake/overgrow bacteria. But overgrown bacteria isn't killed, just stunned. If the popcorn has any bacterial problems at all, the project will be ruined as said bacteria would get injected into the spawn bags. The slurry jar is shaken an excessive number of times (and especially at 100%) to make sure it fully recovers. Popcorn with any bacterial problems will not recover. The bacteria will "bloom" after the shake and take over the jar very quickly. MLB - yeah, I like the side pic better than the top pic. The top view is nice because the canopy is full, but you can't tell how friggin' long and lovely those stems are. Excellent harvest from this project. I am very happy with the results. The only annoying part was starting a hole in the corner to harvest through! The stems were extremely close together. Harvest took almost two hours.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #22 (permalink) |
| A Mirror Image Join Date: Sep 1972
Posts: 4,232
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Those are beautiful happy mushrooms. Way to grow Buckaroo
__________________ Waylit's Exotic Roundup - a collection of sexy threads |
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| | #23 (permalink) |
| suodɐǝʍ ƃuıןıdʞɔoʇs Join Date: Aug 2008
Posts: 632
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Bravo!!! Excellent show Buck! I have a question for you, when you prepare your slurry syringes you mention that you make 3 "pulls" from the jar then what happens with that jar? Do you allow it to recover (if it will) or do you scrap it and make a new jar for your next run. Seems like this might provide you with a somewhat perpetual supply of slurry if the jar keeps going.
__________________ Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. -tool |
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| | #24 (permalink) |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
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Firstly, thanks! Second, you can easily pull 10 60mL syringes of slurry from a popcorn pint. Three syringes is all the jar will hold each time. After you suck it dry, spew in a bunch more water and shake - more slurry comes out! Check this thread: http://forums.mycotopia.net/fungi-gr...ulant-tek.html (Making mycelia slurry syringes - bulk inoculant Tek) This is a pic of the 10th 60mL syringe drawn from a popcorn pint: ![]() Beaucoup myc still present! All three spawn bags came from the inoculant of one popcorn pint. The whole project started as a single popcorn pint turned into slurry. That slurry inoculated the three big popcorn bags, they inoculated the nine big substrate bags. That is why shaking the pint at least 3 times is so important. You are both testing for contamination and building up lots of myc to make the slurry. You can put a pint that has been "slurried" back into the incubator. If you pull enough water out of it, it will recover. But the popcorn gets kinda mushy. If you "slurry" that jar again, you get a lot more bits in the water. Bits tend to clog the syringe when you draw out the slurry.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #26 (permalink) |
| Prone to ranting... Join Date: Oct 2005
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Dude, Eats, you are old. I had that same thought when I was shrinking that pic to post...
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #30 (permalink) | |
| Satan's Helper Join Date: Jun 2008
Posts: 1,349
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | Quote:
Poly is polyfil, or pillow stuffing. It is the white fibrous material that is stuffed in pillows.
__________________ "It was the straying that found the path direct" - Austin Osman Spare | |
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| | #35 (permalink) | |
| -= Magic Passion =- Join Date: Aug 2008
Posts: 791
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | Quote:
EDIT: quick google i was trying to grow mj with that 8 years ago, was fine until forgot to water it few days and it was completely dry. nice thing to see the roots since its transparent... i remember a thread with casing cakes from year ago.
__________________ "when you don`t know where you going any road will take you there..." | |
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| | #36 (permalink) |
| Prone to ranting... Join Date: Oct 2005
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The poly is polyacrylamide gel, just like you picture with the plant roots. I like it because it lets me add large amounts of extra water to a substrate.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) Last edited by BuckarooBanzai; 10-27-09 at 11:33. |
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| | #37 (permalink) |
| Satan's Helper Join Date: Jun 2008
Posts: 1,349
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Ahh, ok. Sorry for the sidetrack Buck but what do you think about adding say 60mL of water to a colonized wheat jar, shaking, then drawing the water back out? Would it work similar to the slurry method but with less equipment?
__________________ "It was the straying that found the path direct" - Austin Osman Spare |
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| | #38 (permalink) |
| Prone to ranting... Join Date: Oct 2005
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The problem with wheat or rye is the small grain size. The syringe tends to clog when you draw the slurry water out.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #39 (permalink) |
| Satan's Helper Join Date: Jun 2008
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What is causing the clogs, the grain itself? Makes sense... so no slurry maker would really be necessary for popcorn? You could just shake and draw?
__________________ "It was the straying that found the path direct" - Austin Osman Spare |
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| | #40 (permalink) |
| Prone to ranting... Join Date: Oct 2005
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The big popcorn kernels tear the myc to shreds just from shaking and they don't break down. The slurry has a tendency to clog just from the volume of myc. Add any little grain bits and it would just clog worse. Also, all the space between the popcorn kernels means you can inject a lot more water into the jar.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #42 (permalink) | |
| Prone to ranting... Join Date: Oct 2005
Posts: 6,050
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | Quote:
I just did - everybody who grows with strawnet is using polyacrylamide. Poly is one of the ingredients in strawnet. I am the only grower I know of who adds poly crystals as a substrate additive unto themselves.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) | |
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| | #45 (permalink) | |
| -= Magic Passion =- Join Date: Aug 2008
Posts: 791
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | Quote:
http://forums.mycotopia.net/fungi-gr...al-casing.html (DE Water Crystal Casing.)
__________________ "when you don`t know where you going any road will take you there..." Last edited by sagiboy; 10-28-09 at 07:07. Reason: damn im old... | |
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| | #46 (permalink) | |
| Addicted to Invitro Join Date: Mar 2006
Posts: 1,862
![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | Quote:
You still use vermiculite for BRF cakes? | |
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| | #47 (permalink) |
| Prone to ranting... Join Date: Oct 2005
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Yep. Verm for my cakes. Poly makes crap cakes and is useless as a contam barrier. It's value is in holding so much water. Makes it very easy to add lots of extra water to a substrate without drowning it. 'Tis why I don't dunk any more. Never saw that thread Sagiboy, thanks for the link.
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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| | #48 (permalink) |
| Addicted to Invitro Join Date: Mar 2006
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I guess there really is no better alternative for vermiculite in BRF cakes. It is just such nasty stuff, but I am a germaphobe and OCD, lol. Thanks for the knowledge as ever, BB. Sidenote: I was walking out of a graduate class last night, and one of my classmates (an old hippie dude) asked me--quite out of the blue--if I had ever seen "The Adventures of Buckaroo Banzai." I answered, quite without thinking, "Yeah, he's a friend of mine." |
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| | #50 (permalink) |
| Prone to ranting... Join Date: Oct 2005
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LOL! Good one, man. Lotsa gigglies on here today. I gotta quit taking antihistamines and drinking beer before 9AM...not today, I mean, but...ya know...tomorrow. Best time in the world to start anything you don't intend to actually do: Tomorrow. ALWAYS put off until tomorrow those things you aren't going to do anyway. Wonderful word, wonderful sentiments. And it always makes me think of the musical Annie and that wretchedly maudlin Charles Strouse song. "I love ya, tomorrow, yer always a day away!" Ya, probably not a great idea to be hitting the bong this early, either. Oh well - it's five o'clock somewhere...
__________________ Banzai Institute for Higher Education (a collection of growing Teks & threads) |
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