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Old 10-03-09, 17:17   #1 (permalink)
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Hillbilly bulk grow in a 25 gallon aquarium

Strain: Hillbilly
Inoculant: Mycelia slurry
Spawn: Popcorn
Spawn container: Medium pre-sealable filter patch spawn bags from sporeworks.com
Spawn Ratio: 1:3 / popcorn:bulk
Bulk Substrate: 40:40:20 / coir:castings:Poly (by volume)
Substrate depth: 4in
Spawn Run Temp: 83F-84F
Pinning/cropping Temp: 75F-80F

Process:
A pint canning jar with silicone injection ports and a double Tyvek vent was filled 2/3 with hydrated popcorn. Three medium pre-sealable spawn bags from sporeworks.com were filled with 10 cups of hydrated popcorn and 1 cup of bulk substrate and sealed with an Impulse sealer. Substrate and popcorn were mixed thoroughly before filling the bags. The spawn bags and pint jar were sterilized at 15psi for 90 minutes

After cooling completely, the pint jar was inoculated with .5mL of Hillbilly slurry, shaken and incubated at 84F-85F. When approximately 70% colonized, the popcorn jar was shaken thoroughly. At 100% colonization the popcorn jar was shaken thoroughly. After the jar was again 100% colonized, it was shaken violently to prepare for mycelia extraction.

180mL of sterile water was injected into the popcorn pint jar. The jar was shaken violently for about 3 minutes to break up the mycelia and distribute it into the water. Using a 60mL syringe, as much water as possible was removed from the pint jar. Approximately 165mL of mycelia slurry was recovered from the pint jar. This process was repeated 3 times to produce approximately 490mL of slurry inoculant.

Each spawn bag was injected with about 160mL of slurry through a silicone injection port. The slurry was distributed across as much of the popcorn as possible and the bags were kneaded thoroughly after inoculation. The bags were then incubated at 84F-85F until colonized 50%. The bags were then kneaded and incubated until colonized 100%.

90 cups of bulk substrate (40:40:20 coir:castings:Poly - by volume) was prepared. All substrate components were hydrated to field capacity before mixing. The bulk substrate was loaded into 9 medium sporeworks.com pre-sealable spawn bags, Impulse sealed and sterilized at 15psi for 90 minutes.

The 100% colonized popcorn spawn bags were thoroughly kneaded to break up all mycelia chunks. After kneading the bags were incubated at 84F-85F for 24 hours to allow time for recovery and test for bacterial contamination.

Popcorn spawn and bulk substrate were mixed in the aquarium with a large spoon. Approximately 10% of the bulk substrate was held back and used as a top dressing, covering all exposed popcorn. The Aquarium was covered with aluminum foil (on top) and incubated at 83F-84F until the substrate was completely colonized.

The foil “lid” had 8 holes in it, each about the size of a quarter. No attempt was made to tape the foil down, it was just press fitted to allow some fresh air exchange. The same aluminum foil “lid” was used for fruiting..

Fruiting was initiated by putting the aquarium in diffuse natural light (no direct sunlight). Fresh air was provided by manual fanning 3 times per day. No automated air exchange was used. No misting was required.

Time frame was 31 days from inoculation to harvest of the first flush.

Pictures of the aquarium (top and side) before harvest:





I love the Hillbilly strain! Such a giver.

Bulk substrates are fun...
Attached Thumbnails
hillbilly-bulk-grow-25-gallon-aquarium-top_view.jpg   hillbilly-bulk-grow-25-gallon-aquarium-side_view.jpg  
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Old 10-03-09, 17:24   #2 (permalink)
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Very nice!!
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Last edited by Om shanti; 10-03-09 at 17:32. Reason: dumb me
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Old 10-03-09, 17:24   #3 (permalink)
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Nice man!!!
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Old 10-03-09, 17:28   #4 (permalink)
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Cool, I see mycelium consuming some shrooms.

I see that with pans mostly. Can't say that any of my cubes have ever done it.
Although I chop mine down a bit sooner than some.
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Old 10-03-09, 17:43   #5 (permalink)
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Overly humid conditions cause that "reversion" growth where the caps turn back into growing myc.

Mr. Aquarium should have been fanned a couple more times each day. Maybe slightly larger breather holes as well.
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Old 10-03-09, 17:45   #6 (permalink)
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That is a full aquarium,very nice BB
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Old 10-03-09, 17:47   #7 (permalink)
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That's awesome. So are we really looking at 90 cups of substrate in that thing? Or did you do something else with the rest of it?
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Old 10-03-09, 17:53   #8 (permalink)
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Nope, everything is in the aquarium. Check the pic. The substrate represents a little less than 1/3 of the aquarium's total volume (about 6-7 gallons of substrate).

6 gallons is 96 cups.

We are so stupid for not having standardized on the metric system. Looking that conversion up on Google was annoying. But how the hell ya gonna remember that a gallon is 16 cups? What kind of goof ball uses base 16 counting? Hexadecimal is for computers and gate based logic. Not people.

So friggin' stupid.
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Old 10-03-09, 19:31   #9 (permalink)
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BB, I aspire to growing a bulk like that...

So, you sterilize your sub instead of pasteurizing?

Also, When you say the foil lid was used in fruiting, do you mean you put the foil over the top of the aquarium so light would only come in from the sides?
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Old 10-03-09, 20:01   #10 (permalink)
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That bulk is worm castings and coconut coir and I sterilize it at 15psi for 90 minutes.

You would not want to sterilize manure or straw, but it can very safely be done with castings and coir.

The only light was ambient/indirect natural light coming in the sides of the aquarium. The whole top was covered with aluminum foil.
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Old 10-03-09, 20:03   #11 (permalink)
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What sterilizing just coir/verm? I have been trying to pasteurize but maintaining a 160 temp is proving to be not easy for me.
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Old 10-03-09, 20:22   #12 (permalink)
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Wow! Nice work. I keep hearing good things about slurry. How fast did the spawn bags colonize? A few days? Did the corn get prepared any differently to compensate for all the liquid that was gonna get pumped into it?
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Old 10-03-09, 20:28   #13 (permalink)
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The spawn bags take about 5-6 days to fully colonize.

A full cup of coir:castings:Poly (the bulk sub) is mixed into each popcorn bag before it is inoculated. That soaks up all the extra water and gets the myc to start producing the enzymes it will need to digest the bulk sub.

You should be able to sterilize coir:verm with no problem at all.
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Last edited by BuckarooBanzai; 10-04-09 at 09:59. Reason: subject/verb agreement
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Old 10-04-09, 00:26   #14 (permalink)
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Very nice BB, I would love to have mine look like that.
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Old 10-04-09, 00:30   #15 (permalink)
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I'd like to walk through that mushroom forest.

Very nice work Buckaroo!
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Old 10-04-09, 01:10   #16 (permalink)
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BB your work is inspirational to me. Very awesome and easy to follow.

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Old 10-04-09, 05:53   #17 (permalink)
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That is one of the most impressive pictures I have seen in a while, a dense forest of mountain folks! Virgin timber indeed!
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Old 10-04-09, 06:14   #18 (permalink)
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BB that looks badass
i hope i can do that good
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Old 10-04-09, 07:04   #19 (permalink)
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Wow. That's one of the most impressive bulk grows I ever seen. That's very big mushrooms you have there and a beautiful canopy. As mentioned, very inspirational. Thanks for sharing the pictures and info.

Why do you shake the popcorn jar one more time after it's 100%?
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Old 10-04-09, 09:13   #20 (permalink)
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Jeeeeeeeeeeeezzzzzzzz
Awesome werk BuckarooBanzai
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Old 10-04-09, 09:48   #21 (permalink)
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Why thank ya'll kindly. I am just applying the lessons I learned here at Mycotopia University. Any one of you could do this exact same project at home. Popcorn and bulk substrate are easy to prepare (or order from Mycrotopia).

The popcorn jar is turned completely into mycleia slurry by flooding it with sterile water and shaking it violently (multiple times).

Mycelia grows so fast on popcorn that it can overtake/overgrow bacteria. But overgrown bacteria isn't killed, just stunned. If the popcorn has any bacterial problems at all, the project will be ruined as said bacteria would get injected into the spawn bags.

The slurry jar is shaken an excessive number of times (and especially at 100%) to make sure it fully recovers. Popcorn with any bacterial problems will not recover. The bacteria will "bloom" after the shake and take over the jar very quickly.

MLB - yeah, I like the side pic better than the top pic. The top view is nice because the canopy is full, but you can't tell how friggin' long and lovely those stems are. Excellent harvest from this project. I am very happy with the results.

The only annoying part was starting a hole in the corner to harvest through! The stems were extremely close together. Harvest took almost two hours.
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Old 10-04-09, 11:30   #22 (permalink)
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Those are beautiful happy mushrooms. Way to grow Buckaroo
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Old 10-04-09, 14:04   #23 (permalink)
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Cool

Bravo!!! Excellent show Buck! I have a question for you, when you prepare your slurry syringes you mention that you make 3 "pulls" from the jar then what happens with that jar? Do you allow it to recover (if it will) or do you scrap it and make a new jar for your next run. Seems like this might provide you with a somewhat perpetual supply of slurry if the jar keeps going.
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Old 10-04-09, 14:23   #24 (permalink)
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Firstly, thanks!

Second, you can easily pull 10 60mL syringes of slurry from a popcorn pint. Three syringes is all the jar will hold each time. After you suck it dry, spew in a bunch more water and shake - more slurry comes out!

Check this thread:
http://forums.mycotopia.net/fungi-gr...ulant-tek.html (Making mycelia slurry syringes - bulk inoculant Tek)

This is a pic of the 10th 60mL syringe drawn from a popcorn pint:


Beaucoup myc still present!

All three spawn bags came from the inoculant of one popcorn pint.

The whole project started as a single popcorn pint turned into slurry. That slurry inoculated the three big popcorn bags, they inoculated the nine big substrate bags.

That is why shaking the pint at least 3 times is so important. You are both testing for contamination and building up lots of myc to make the slurry.

You can put a pint that has been "slurried" back into the incubator. If you pull enough water out of it, it will recover. But the popcorn gets kinda mushy. If you "slurry" that jar again, you get a lot more bits in the water.

Bits tend to clog the syringe when you draw out the slurry.
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Old 10-04-09, 14:39   #25 (permalink)
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nice! that side shot is awesome! reminds me of that old video from homestead that came with the kit they used to sell. lol..
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Old 10-04-09, 14:55   #26 (permalink)
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Dude, Eats, you are old.

I had that same thought when I was shrinking that pic to post...
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Old 10-26-09, 12:06   #27 (permalink)
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Just fantastic!

Congratz
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Old 10-26-09, 14:52   #28 (permalink)
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what is poly?

Banzai Institute for Higher Education stuff indeed.

how long have you been planing to use that aquarium? hehe...
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Old 10-26-09, 15:16   #29 (permalink)
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that side shot is friggin EPIC!!!!!!
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Old 10-26-09, 15:19   #30 (permalink)
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what is poly?

Banzai Institute for Higher Education stuff indeed.

how long have you been planing to use that aquarium? hehe...

Poly is polyfil, or pillow stuffing. It is the white fibrous material that is stuffed in pillows.
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Old 10-26-09, 16:09   #31 (permalink)
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as substrate?
never heard of it... just pasteurize with the coir/humus?
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Old 10-26-09, 16:40   #32 (permalink)
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Awesome Pic BB very nice informative grow log
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Old 10-26-09, 16:45   #33 (permalink)
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Anyone else notice the little guy stuck in the mycelium to the right of the lighter? Poor little guy
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Old 10-26-09, 20:11   #34 (permalink)
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Poly is polyfil, or pillow stuffing. It is the white fibrous material that is stuffed in pillows.
Not in this case. BB uses polyacrylamide crystals instead of vermiculite. He is the only grower I know of who uses them.
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Old 10-26-09, 21:06   #35 (permalink)
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Not in this case. BB uses polyacrylamide crystals instead of vermiculite. He is the only grower I know of who uses them.
some info on that BB?

EDIT: quick google

i was trying to grow mj with that 8 years ago, was fine until forgot to water it few days and it was completely dry. nice thing to see the roots since its transparent... i remember a thread with casing cakes from year ago.
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Old 10-26-09, 23:50   #36 (permalink)
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The poly is polyacrylamide gel, just like you picture with the plant roots.

I like it because it lets me add large amounts of extra water to a substrate.
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Old 10-26-09, 23:57   #37 (permalink)
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Ahh, ok. Sorry for the sidetrack Buck but what do you think about adding say 60mL of water to a colonized wheat jar, shaking, then drawing the water back out? Would it work similar to the slurry method but with less equipment?
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Old 10-27-09, 00:13   #38 (permalink)
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The problem with wheat or rye is the small grain size. The syringe tends to clog when you draw the slurry water out.
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Old 10-27-09, 00:21   #39 (permalink)
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What is causing the clogs, the grain itself? Makes sense... so no slurry maker would really be necessary for popcorn? You could just shake and draw?
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Old 10-27-09, 10:36   #40 (permalink)
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The big popcorn kernels tear the myc to shreds just from shaking and they don't break down.

The slurry has a tendency to clog just from the volume of myc. Add any little grain bits and it would just clog worse.

Also, all the space between the popcorn kernels means you can inject a lot more water into the jar.
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Old 10-27-09, 11:26   #41 (permalink)
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Thank you so much bucky!
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Old 10-27-09, 14:51   #42 (permalink)
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Not in this case. BB uses polyacrylamide crystals instead of vermiculite. He is the only grower I know of who uses them.
That comment bugged me. But I couldn't remember why.

I just did - everybody who grows with strawnet is using polyacrylamide. Poly is one of the ingredients in strawnet.

I am the only grower I know of who adds poly crystals as a substrate additive unto themselves.
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Old 10-27-09, 20:43   #43 (permalink)
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Old 10-27-09, 22:12   #44 (permalink)
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That is amazing!!!! Gives me alot of motivation toward bigger goals!
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Old 10-28-09, 06:59   #45 (permalink)
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That comment bugged me. But I couldn't remember why.

I just did - everybody who grows with strawnet is using polyacrylamide. Poly is one of the ingredients in strawnet.

I am the only grower I know of who adds poly crystals as a substrate additive unto themselves.
remember this one?
http://forums.mycotopia.net/fungi-gr...al-casing.html (DE Water Crystal Casing.)
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Old 10-28-09, 07:04   #46 (permalink)
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That comment bugged me. But I couldn't remember why.

I just did - everybody who grows with strawnet is using polyacrylamide. Poly is one of the ingredients in strawnet.

I am the only grower I know of who adds poly crystals as a substrate additive unto themselves.
Ah, I did not know that about strawnet.

You still use vermiculite for BRF cakes?
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Old 10-28-09, 08:58   #47 (permalink)
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Yep. Verm for my cakes. Poly makes crap cakes and is useless as a contam barrier.

It's value is in holding so much water. Makes it very easy to add lots of extra water to a substrate without drowning it. 'Tis why I don't dunk any more.

Never saw that thread Sagiboy, thanks for the link.
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Old 10-28-09, 09:39   #48 (permalink)
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I guess there really is no better alternative for vermiculite in BRF cakes. It is just such nasty stuff, but I am a germaphobe and OCD, lol.

Thanks for the knowledge as ever, BB.

Sidenote: I was walking out of a graduate class last night, and one of my classmates (an old hippie dude) asked me--quite out of the blue--if I had ever seen "The Adventures of Buckaroo Banzai." I answered, quite without thinking, "Yeah, he's a friend of mine."
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Old 10-28-09, 09:47   #49 (permalink)
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[MOD EDIT]
{may i share this mods?}

No!
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Old 10-28-09, 09:51   #50 (permalink)
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LOL! Good one, man. Lotsa gigglies on here today. I gotta quit taking antihistamines and drinking beer before 9AM...not today, I mean, but...ya know...tomorrow.

Best time in the world to start anything you don't intend to actually do: Tomorrow. ALWAYS put off until tomorrow those things you aren't going to do anyway.

Wonderful word, wonderful sentiments. And it always makes me think of the musical Annie and that wretchedly maudlin Charles Strouse song. "I love ya, tomorrow, yer always a day away!"

Ya, probably not a great idea to be hitting the bong this early, either.

Oh well - it's five o'clock somewhere...
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