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10-28-09, 12:26
| #1 (permalink) |
| Mycotopiate  Join Date: Nov 2007
Posts: 217
  | PESA bulk
Foaf has been working with some pesa isolates. it began from one drop of multispore on popcorn. one kernel was selected and put on agar then selections were made plate to plate for the most rhizomorphic growth. agar to master jars of rye berries and g2g up to 3 generations. the substrate for these monotubs are straw/coir/strawnet/verm with some horse poo and castings. steam sterilized in a 55 gal drum. The two monotubs have no casing and the double tub is cased with coir/verm that was sterilized in a pc in filterpatch bags. foaf makes 1 1/4 inch holes, 3 per side of tub and polyfills. the pictures were taken maybe the 4th or 5th day since the first primordia was noticed. the temp of the room the tubs are in is 71 degrees.
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10-28-09, 12:51
| #3 (permalink) |
| Void Of Boundaries  Join Date: Jul 2009
Posts: 578
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Beautiful work brother!!!!!!! Cannot wait to see the canopy. hehe. make sure to post a report and the weight of the flush! I just like the dirty little details i guess!!!!!
__________________ "LEARN THE RULES SO YOU CAN BREAK THEM PROPERLY" -The Dali lama "i don't care if it eats my cat, as long as the damn thing fruits"~ prism |
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10-29-09, 11:48
| #8 (permalink) |
| Mycotopiate Join Date: Nov 2007
Posts: 217
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Thank you all for the kind words. heres some details. 6 quarts of rye berry spawn inoculated the 50 and 64 quart monotubs. 8 quarts of rye berry spawn inoculated the dub tub. sometimes foaf mixes spawn and sub together, sometimes foaf does layer spawning, sometime a mix of the 2. the sub depth is anywhere from 3-6 inches depending on the bin used (taller tubs,more substrate depth). the 1st monotub(first 3 pictures) is about 5 inch sub and the 2nd monotub (2nd set of 3 pics) is 3.5 to 4 inch. The dub tub(3rd set of 3 pics) is 6 inches with maybe a 1/4 to 1/2 inch casing. the last pic is pesa p4d2 #1 clone spawn, the tubs are all p2a or p2b. foaf has fruited out the p4d2 isolate but has not fruited out the #1 clone yet. foaf is expanding the clone atm and is on g2. foaf used 2 of the #1 masters to get the ball rolling quicker and has 4 masters left in the fridge. the tubs haven't changed too much from yesterday as the room temps are at 70 now were a few degrees cooler during the night. the pins that were already there are slightly larger and some more pins formed. foaf will keep taking pictures of the 3 tubs pictured and will update when growth is more evident. heres a couple pics taken yesterday that should keep you guys tied over till more pinshots. these are examples of tubs that are still colonizing. the first picture is pure straw/rye spawn layered with a layer of straw on top and the 2nd picture is layered but spawn is the top layer. foaf likes the look of the 1st pictured tub better. the mycelium has more upwards momentum, which foaf thinks is a good thing. either way foaf makes sure to cut or rip out stray peices of straw that stick up before fruiting is initiated. both methods have there merits but foaf is leaning towards the layer of sub on top if you have a smooth sub(no straw sticking up an inch off the sub) and topspawning if your sub has high percentage of straw to make the sub flat. what does everyone else mix/lay out there spawn and subs and their top layer and why?  |
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11-02-09, 09:46
| #16 (permalink) |
| The green paladin  Join Date: May 2008
Posts: 406
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looking great .. look like you need to harvest the second tub ....   peace zozo
__________________ My Grow logs |
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11-02-09, 11:02
| #22 (permalink) |
| Mycotopiate Join Date: Nov 2007
Posts: 217
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Thank you everyone for visiting this thread. Eatyualive- Strawnet works great. All foaf did was moisten and crumble up and mixed into coir/straw/verm/hpoo. Foaf didn't use in too high of proportion bc didn't remove binder. The 3rd(dub)tub has not matured yet. The pics are up to date. Foaf has been running cooler temps hoping that the fruits will be more dense after foaf saw a buddy test run that ran the same pesa gifted by foaf in the 18 gal roughneck that yielded 8 zips dry first flush(ruined the casing and it contamed pre 2nd flush). pure straw that was compressed(stood on sub using a bin on top of a bin with garbage bad protecting sub) and cased with pure coir after colonization. very thick sub with 8 quarts rye berries layered spawn. the dude was running 68-70 degree fruting temps and the fruits were not hollow at all and were super dense. foaf did get a clone from the 8 zip tub but has not tested it yet. foaf just poured 40 agar plates and will be expanding the clone shortly. TheFreak- Sporeworks- foaf has only good things about them. never had a contamed syringe/print from this particular sponser. marmy- thankyou, foaf isn't doing anything new. foaf thinks eatyualives thread on monotubs and dubtubs is great read-everything you would want to know about tubs. best advice foaf can give is to get your bulk sub to the correct moisture content(can't stress enough)-not to wet, not too dry.best to be a little under perfect moisture than under.harder to remove moisture than add. when foaf squeezes bulk sub no drops. if a tub looks too dry foaf can add a casing or spray the sides. if a sub is too wet get too much condensation and sub is easy to contam. |
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11-02-09, 11:43
| #23 (permalink) | |
| ExoCannibalist  Join Date: Jan 2006
Posts: 5,064
| Quote:
how deep is the sub? i might go 5 quarts or more next few tubs. i pulled 12z dry off 5 quarts and about 3" sub depth. but im not sure my tubs would hold 8 quarts spawn plus the bulk subs placed in it. 2nd flush was 6, 3rd turned out to be close to 3. was great yield. also used some supercake in that flush.
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11-02-09, 12:04
| #24 (permalink) |
| riding the roller coaster  Join Date: Oct 2008
Posts: 836
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I liked how you were trying different layering of the spawn. I have seen the dump and mix method... works pretty good, but no even pinsets everytime... or at least from multi generational G2g... more even from 1st gen multispore...but not tested enough to make a statement to that fact.
__________________ OK maybe not that old |
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11-02-09, 17:09
| #28 (permalink) |
| Mycotopiate Join Date: Nov 2007
Posts: 217
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Eatyu- the compressed straw sub in the 18 gal roughneck was 9-10 inches plus the casing when foafs buddy got 8 zip first flush. the tub is a total of 20 inches tall and the fruits were hitting the top, using up all the space in the tub, full canopy flush. 6 qts layered with straw and compressed, then 2 quarts dumped on top, then cased in 3-4 days. 2 inch holes with postal tyvek was used for air exchange.
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11-02-09, 21:11
| #33 (permalink) |
| The Seeker  Join Date: Nov 2007
Posts: 285
| !!!Holy B'Jebus!!!! Mad Props Indo
__________________ "Why is it," he said, one time, at the subway entrance, "I feel I've known you so many years?" "Because I like you," she said, "and I don't want anything from you." — Ray Bradbury (Fahrenheit 451) |
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11-04-09, 09:15
| #37 (permalink) |
| Mycotopiate Join Date: Nov 2007
Posts: 217
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Foaf layers spawn so the mycelium has to run upwards. the myc seems to gain "pinning momentum" as it runs. sometimes foaf will throw in 4 jars with x amount of sub and just mix up and then layer with 2 jars, half a jar per layer. with spawn on top the tub can be cased sooner and colonizes quicker, (foaf always cases when the layer of spawn on top, forcing the mycelium to run) than if the sub is on top bc the spawn has to run up through it. when the spawn runs upwards and hits the top(whether its sub or casing) at mostly the same time you end up with super rhizo myc that leads to a massive pinset. either mixing or layering will work and can give massive pinsets, its just that when you get myc to run up it almost primes it for pins.my foaf will get pins with PESA in as little as 1 day from inducing pinning conditions. keeping subs in the dark until colonization is another way to prime the mycelium for pinning..and layering spawn under sub will help keep the myc from seeing the light until the myc hits he top of the sub.there is a window of a few days to take other projects from the same dark area before the layered spawn hits the top of the sub. when the spawn is layered, the spawn seems to take off faster than mixing bc the myc isn't handled as much as when you mix spawn and sub all together.if you can break up your grain jars enough, you wont have to touch the spawn at all, just lay it out and layer sub on top without touching. the good part about layering spawn on top is that if any of the top spawn were to contam, you can sprinkle healthy spawn of the same isolate on top of the contam and if its enough spawn it will hopefully isolate the contam and possibly keep the contam from spreading. with spawn layered on top if there is a contam in the middle of the sub, the layer of grain on top acts like a shield and can still get a flush. foaf has been seeing the best uncased results with at least one layer of spawn and a thin even layer of spawn on top. the bottom doesn't have to be layered, you can just mix. |
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11-04-09, 15:34
| #38 (permalink) |
| Slaptastician  Join Date: Feb 2005
Posts: 1,319
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^^^^ Good stuff. I've started putting a layer of substrate on top and I think it helps as well. Usually I just mix spawn in with the rest of the sub, but I can see how handling it more gently by layering would allow it to recover more quickly. How do you suppose keeping things in the dark helps pinning? |
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11-04-09, 19:15
| #39 (permalink) |
| Areoponic Chronic  Join Date: Nov 2006
Posts: 193
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ya but you still have to break it up quite a bit if you are using jars just to get it out. I usually break into individual kernals and mix it with sub I was just wondering if a top spawn layer helps with pin set at all also indonesia how long is foaf spawn run before pinning condition introduced with top spawn layer? and does foaf put casing layer on at spawn or after top layer is recovered?
__________________ Mycelium is yourcelium |
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11-05-09, 08:43
| #40 (permalink) |
| Mycotopiate Join Date: Nov 2007
Posts: 217
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sidestreet getaway- keeping tub in dark helps to give it a jumpstart when foaf wants pinning conditions begin(full colonization with uncased or after mycelium shows in casing layer evenly across the casing. even a half a millisecond of light can have a stimulating effect on cubensis, and don't want to stimulate until its ready to pin or you may have stray pins rather than an even pinset. part of foafs pin initiation strategy is light, the rest is intro of fresh air and temp reduction(if possible). TrickyNicky- My foaf uses jars and breaks up with a tire and shakes into individual peices..sometimes get a clump but no big deal. A top spawn layer can help with pinset by keeping straw laying flat and making sub lay flat and if you lay an even casing on top. spawn run is same as top sub layer, maybe a day sooner(can be as little as 3 days, depends on substrate temp). foaf usually waits for subs colonization before laying out a casing. foaf has been doing some trials recently laying out the casing at spawning over a topspawned tub to try to have a fully colonized casing layer by the time the substrate is at full colonization. foaf was a little worried that the casing may contam from laying it out over freshly broken up spawn, but so far so good. my foafs favorite spawn method is currently mix 4 jars with a 3-4 inch sub then- half jar spawn,thin layer sub(just enough to cover up spawn), half jar spawn, thin layer sub, then whole jar, and a lil thicker layer of sub on top. |
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Beautiful.. cant wait to see the results! G/L
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