fungi Archive through January 20, 2005

Lets say, for a typical clone setup (mushroom piece in jar with brf and h202/h20 (40% solution))how many syringes, or how much h202 should I use per 1/2pt jar? also, how much brf per jar should I add? I know I only gotta add a little piece of flesh, but what would be the best part to choose, cap, stem?

Thanks,
Tele

[nomoreusmc]

most people use the inside fleash from the stem

<u>How about this</u>?

Clean a blender out with alcohol.
Soak a whole mushroom in peroxide a few minutes.
Throw it in blender with some sterile water.
Liquify.
Suck up into your syringe.
Draw a few cc's peroxide into syringe.
Shake, and let it foam up.
Innoculate as usual.

I have done this twice with excellent
results. No glovebox or precautions.

Maybe I have been lucky.

hmm...

Sounds pretty good, actually easier than what I had planned on doing...In the tek I was reading, the plans weren't too specific. But, I guess it's really not that complicated. Thanks.

Tele

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

Throw it in blender with some sterile water<!-/quote-!><hr size=0></blockquote>

you may find that a widemouth pint or half-pint
jar will screw into your blender blade assembly
base nicely.

I did some Pesa cloning on x-mas day,and didn't use any Peroxide...
Used the inside of the stem,and blended it with distilled water.

This is how it looks after 3 days:


No contam.

Thats interesting --bypassing the whole Karo jar thing....I might have to try that...
Any one else have luck with tec..???

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

Soak a whole mushroom in peroxide a few minutes<!-/quote-!><hr size=0></blockquote>

You might want to omit the cap. You don’t want any spores in the liquid if you’re cloning.

ooo Good point!

Nice pics...Def. gonna have to try it...

Now when we say "the inside of the stem", are we talkin' splitting, then scoopin out the flesh on the inside? like, the middle gunk?

(Message edited by teletrue on December 28, 2004)

(Message edited by teletrue on December 28, 2004)

yeah, but be sure to split the cap w. ur hands, ripping it, not cutting it, and taking a piece from the clean middle

narrowmouth jars fit nicely onto my blender base
i took a medium sized stem, dropped it in h2o2
and it fizzed for about 3 minutes. I then took
the stem and dropped it into a narrowmouth pint
jar with about 1/2 a pint of tap water and 20cc
of h2o2. Liquified and then drawn back into the
syringe. I have innoculated a case of pints to
look for contams.

An interesting effect, with cubes, after blending
the water will slowly begin to turn blue.

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

You might want to omit the cap. You don’t want any spores in the liquid if you’re cloning.<!-/quote-!><hr size=0></blockquote>
Right on.

And the ripping, instead of the cutting, that signals new growth, right? like, scraping a casing after a flush?

I think the ripping is only done to expose the uncontaminated inside of the fruit, without touching it. Less chance of contams.

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

I think the ripping is only done to expose the uncontaminated inside of the fruit, without touching it. Less chance of contams.<!-/quote-!><hr size=0></blockquote>

Yes,that the whole idea...
And the upper stem is the youngest bit of the shroom...,compare it to a plant/tree

ahhh. Thank ya!

That's interesting, have you noticed a difference in performance between the upper and lower stem?

wait wait wait! does a shroom grow up from the bottom of the stem? or does it grow from the top?

Didn't try the lower stem yet...
The upper stem looks younger,but I do not have proof on that.
I think you have to draw a line on a young mushroom, to see what happens when it grows !


(Message edited by spacecake on December 29, 2004)

These little vials work great for liquid culture!

They can be labeled and easily stored in the fridge for months. They can be purchased thru medical supply outlets, and work great because they provide a self healing injection site, keeping the contents inside sterile.



This is standard liquid culture in smaller containers. A mix of water and Karo, honey or corn sugar is used to prepare the vials.

Inject 1 cc of spore solution into each vial thru the self healing injection site.
Alternatively, instead of spores, mycelial tissue can be substituted.

Within several days, strands of mycelial tissue will appear and begin to grow. Allow it to grow at least several days then it is ready to use. You can draw up syringe-fulls through the injection site. Try to get as much tissues as possible in the syringes, a nice thick mix will grow out very fast when used to inoculate jars, etc.

Each vial will make four 10cc syringes from 1cc of spore solution.

Strangeland

Did the vial survive the PC?

(nice find!)

(Message edited by suckerfree on January 02, 2005)

Yes, the vials with plastic lids can be pressure cooked.
They're made with heat resistant plastic,
so they can be autoclaved and shipped sterile.

Another option is to use the metal lid vials.
These are slightly more expensive,
but will last a lifetime.

[troutlips]

What is their capacity? How many ml?

I've found vials between 5-100 milliliters

1 ml = 1 cc (cubic centimeter)

10 ml 20 ml 30 ml
50 ml 100 ml

[sparky]

gota link?

http://www.allergylabs.com/productsearch.asp?produ ctbox=Empty%20Vials

http://www.antigenlab.com/

Thanks!

awesome. great find.

wont the mycelium need some air when growing inside the
vial or is the air left inside sufficiant?
peace ohm

Performed bioassay of eight month-old extract (n=2). There is no noticable loss of potency at this time point. Storage was at -4 deg. C, so the thick layer of precipitated crystals had to be redissolved using a double-boiler on low heat and vigorous shaking.

Next timepoint will be at one year plus.


Shaman

[candykid420]

Thank you

gotta love science -How easy would it be to remove a single dose without cooking up the whole crystal/liquid jug...?

When you throw those vials in the fridge, the mycelium's metabilism slows down almost to a stop, so the amount of air needed is negligible. They last months in those vials, so it must be sufficient.

[tobynutz]

wont the lids blow off in the pc? i have some with rubber lids that pop off that i would like to use, but i would be afraid of the lids shooting off in the pc

EXXXXXXCelent!! Shaman...Did you have an initial loss of potency compared to the number of dry grams you started with? And how much alcohol is the magic sitting in? 190 proof or less?

That is WAY cool. I think this will keep me from taking it all the way dry ever again, unless it is to be used immediately.

Here's some more ideas...

These screw on plastic lids below can be fit with a septa disc,
which is a self sealing silicone disc.

The disc goes inside the lid and screws
onto the top of the vial creating a seal.

The question is...can it go in the PC?
If so, these might work great for liquid culture.





Below are the standard aluminum crimp tops used on serum vials.
Available with rubber or silicone injection sites.


I haven't tried this type yet,
will silicone and rubber melt in the P.C?

Also there are Sepcaps which are special tops fit for water analysis.
They are designed to be used with a syringe.



This site has some interesting products.
http://www.nationalscientific.com/pr...to_shell1.html

After reading the posts here, thought I would drop this in.........slp/fmrc
A...............How To Make "Mushroom Liqueur"
Copyrighted Material taken from #04 "TEONANACATL", The International
Journal Of Psychoactive Mushrooms (TEO) May 2003.
Released to Public Domain by Author and Inventor, S.L. Peele,
Curator FMRC since 1972

This is an excellent way to capture the taste, essential oils, and
all other compounds in any mushroom. The "Mushroom Liqueur" has many
uses. It can be used to extract medicinal properties in cases of
Maitake and Shiitake and provide one with an end product that just
about meets pharmaceutical grade! It can also be used to capture that
special taste of your favorite mushroom like Morels or Chanterelles.
Just add the amount of "Mushroom Liqueur" right for your taste. Leave
dish on low heat and uncovered for just a short time. This will distil
off the alcohol, leaving everything else in the dish, just as it was
in the mushroom. It is unknown how long "Mushroom Liqueur" will hold
and keep in original state, all of the mushroom compounds. "Mushroom
Liqueur" held at room temperature for 5 and 1/2 years, still held fast
all mushroom compounds. This was a sample made from Psilocybe
cubensis. No change in "activeness" was noted. It is possible that
whatever the time limit would be, this could be increased by
refrigerating or freezing.

If you wish to prepare standards for Spectrophotometers, and use these
cells to identify species of mushrooms, use Reagent Grade Ethanol
instead of Rum or other whiskey.

Take 1 pint Bacardi Rum (375ml), you may select other brands or types
of liquor, but keep the alcohol content at least 40% (80 proof) and not more than 45% (90 proof). If a clear type is used, and mushrooms contain
Psilocybin/Psilocin, it may be bluish after the process, even if no
spores are present or other mushroom colors. If wild mushrooms, or
mushrooms bought at the store are used, this will make the end color
very dark in most cases. The mushroom spores will cause this. A
black tint from Panaeolus species, Purple-black from Psilocybe
species, Brown from Agaricus species, and so on. These will settle to the bottom with time.

Supplies Needed: One Pint Bacardi Rum. 600 grams fresh mushrooms
(fresh will always work better, but if dried mushrooms are used, use
60 grams). A large glass vessel that will hold the mushrooms plus the
pint of Bacardi Rum. This vessel must have a cap that will seal
tight. A strainer of some type.

Slice mushrooms up in 1/4 inch long pieces (breakup dried mushrooms).
Try not to make small pieces or powder up dried mushrooms. The Rum
will extract just fine without having small pieces. Having no small
particles will make the filtering easier. Place sliced mushrooms in
vessel, add Rum, seal with cap. Shake gently to insure mushrooms are
in contact with Rum. Let stand one day. Open, and with a wooden
spoon, or something similar, mash the mushrooms and stir them around.
Seal back cap. Do this for 3 more days. On the 5th day, pour out the
solution through a strainer. Squeeze out slices of mushrooms to get
what they are still holding. Place "Mushroom Liqueur" in a bottle
that has a good sealing cap. Make any notes, date, and other remarks on a label attached to the bottle. This way you can identify each
batch of "Mushroom Liqueur" you make. Also be aware that making
"Mushroom Liqueur" with mushrooms that contain controlled substances
such as Psilocybin/Psilocin, may be illegal in many areas, USA, even
in some areas where there are no laws on the possession of fresh said
type mushrooms. Also be aware that the 1 pint rum and 600 grams of
mushrooms used here will yield around a 12 dosage bottle, when
Psilocybe cubensis is used. Having "Mushroom Liqueur" that contains any controlled substances, such as Psilocybin or Psilocin, is illegal where said controlled substances are illegal. No laws are known to exist anywhere when
"Mushroom Liqueur" is made from edible or medicinal mushrooms. Only
alcohol laws that may concern minors may be applicable. This article
is done on an International Basis. Always check with local laws if
you are not sure about the legalities in your area, about anything.
It would be best to do
Page 53 #04 TEO May 2003 Copyrighted Material

this from a pay-phone. Many times the officer you speak with on the
phone may not know anything about mushroom laws. You could get
someone who would say "No, there are no laws on mushrooms", or "No,
there are no laws on that". If you ever get a reply like this, or one
that is in favor of what you are calling about, get the officer's name
and Badge Number, make note of the date and time the call was placed.
If any problems ever do come about, the police where you called will
have a taped conversation, easy to look up with the date and
time....and the officer's name and Badge Number. The term "Mushroom
Liqueur" is hereby viewed as a new mycological term and is Copyrighted
as such in #04 "TEONANACATL", The International Journal Of
Psychoactive Mushrooms (TEO), May 2003, Published by FMRC, POB 18105,
Pensacola, FL 32523. The author hereby places this article under
Public Domain when freely passed on in it's entirety from A - Z.
Stephen L. Peele, Curator FMRC - Z

Stephen, I have read this before. Thank you for the repost. You make the statement:

keep the alcohol content at least 40% (80 proof) and not more than 45% (90 proof).

Could you please expound about the upper limit. It has been proposed that 190 proof everclear is the best to use, and others have used 153 proof liquer (highest proof to be found in some areas) with good effect. Why 90 proof?

And by the way...this will likely be my 800th post! Not that that means anything....LOL

800 thats alot of cartoons Shed .LoL...I was wondering the same thing...I'm guessing that thru comparative trial extractions that the higher water content enabled more compounds to be unlocked.. Maybe the higher alcohol % targets the "active" ingredients more....I could be full of shit though.

Golly, probably only about 500 cartoons really. With the occasional repeat and some posts, like this one when I am tired and have a couple in the thread already don't get one. Theoretically someone could be ultra stealth by getting really good at this hobbie and then the best thing to do would be to do one or two large grows, make extractions out of several pounds, store the extract and then NOT grow for several years. Minimal legal problems that way.

Yeah ..but for me the growing part is half the fun ...Me like fun

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

-How easy would it be to remove a single dose without cooking up the whole crystal/liquid jug...?<!-/quote-!><hr size=0></blockquote>

I redissolved the precipitate to ensure accurate and even dosing. This is not absolutely necessary though, just ask Roo about the bottom of the bottle...lol

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

Did you have an initial loss of potency compared to the number of dry grams you started with? And how much alcohol is the magic sitting in? 190 proof or less? <!-/quote-!><hr size=0></blockquote>

The discussion about ethanol concentration and extraction efficiency is intriguing. I used Everclear for my extraction. I also did not use heat, essentially doing what slp describes above. I suspect about a 75-80% extraction efficiency in the first wash based purely on subjective assessment (note that this is lower than my previous assessment). This loss is tolerated because of the benefits of dosing the extract. I suspect water does indeed extract some of the proteins which can upset the stomach, making 190 proof the ideal solvent for minimizing the extraction of non-magical chemicals.

This ethanol extraction is not for everyone. If shrooms don't upset your stomach, eat freshies, or make tea, to have the most powerful experience. My personal favorite choice is making a tea from freshies. However, when you gotta get rid of a pound of dry shrooms quick-like, the alcohol extraction is handy.

Psilocybin is a water soluble compound. This is why making a tea works so well. Some compounds in mushrooms are water soluble, others are not. To get everything, there must be a ratio of both. The ultimate extraction solvent is 50% Methanol, and 50% Methylene Chloride. This gets everything. Nothing can do better. As Methylene Chloride makes this mixture toxic, the project must be reduced to crystals or tar to rid of this. It would probably be best if no one here was to use Methylene Chloride. This is the way I prepared the mushroom extraction samples for the National Cancer Institute......slp/fmrc

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

To get everything, there must be a ratio of both.<!-/quote-!><hr size=0></blockquote>

Makes sense to me -
As has been mentioned above, the water probably
extracts whatever makes my stomach do flips,
so it's 190 % for me

So then does anyone know what the maximum concentration u can have in an everclear extraction ,where the crystals do not precipitate out ..And u can just simply pour out the dose u want without remixing..?

[insight]

Today makes 22 days since innoculation. I have dunked the cakes overnight for 22 hours in tap water (as I've read tap water is fine for dunking). This evening, 4 were double-end cased, the other 4 set on top of the perlite (no peroxide). I noticed all have what looks like a pale blue color on them in areas. Is this mold or bruising? Is this common?

it's just bruising from handeling the cake... tis all good

Yep...So here's where those pics went! ...they were in a thread in Photo Gallery originally.

However, you may want to get some jar lids or pieces of foil under those cakes and off the Perlite, especially since you didn't treat the stuff with Peroxide.

Oh, and Freaked-Out's avatar is not a doctored photo! The video it was taken from is somewhere on prisonplanet.com I'll look for the link in a bit...funny shit!

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

Oh, and Freaked-Out's avatar is not a doctored photo<!-/quote-!><hr size=0></blockquote>

hah... so true.

At least our president doesnt take any shit

From my experience, about 1 gram per 10 ml of extract.

Potency will vary this - my super PR's follow the above guideline,
while the weaker B+ will go almost up to 2g / 10ml.

Your mileage may vary