fungi Archive through January 28, 2005

Thanks for the tips yall, and once more if anybody's got tips on which pan strains are vigorous...

I guess we'll just have to wait and see what happens when my foaf gets off his lazy tucus and does something with it. He's kind of paranoid, but I'll try for pictures if they happen.

[bob]

Looks nice something I might try. I thought you didn't do cakes?

i did two different strains, Texas and Koh Samui Super strain...

All on the same day, both WBS rez effects, same substrate depth, same casing depth, same moisture, cuz it was from the same bowl of verm...both casings are straight verm

the texans are going beautifully, I cant even see any MYC colonizing the casing, just mushrooms pushing up from under the verm

but the Koh Samui Supers are INSANELY overlaid, it looks like a flatcake, with shrooms growing from the overlaid casing

Both casings are pinning nicely, and im getting a good amount of shrooms, but misting the KSSS seems to leave a puddle, instead of being absorbed.
Does this strain mean that it vigorously colonizes anything? I really like the KSSS, and its gotta be up there in my book, very good stuff....

(Message edited by busst on January 26, 2005)

[kevinfergo]

i just pc'd a few jars (brf/verm) i filled the jars all the way to the threads not packing it in and topped off with dry verm. After pc'ing there is a gap at the top of the jar. the substrate shrunk or got packed somehow. could i put a coffee filter on top to keep out contams and if so does a filter allow enough AE???

Used coffee grinds. These are the first two cakes and probably the last two cakes I'll ever do. I just wanted to see if something other than BRF would work using the basic pf tek. Those were only boiled, never pc'd. I would consider the gypsum necessary. Calcium is a necessary ingredient for fruitbody development and coffee isn't as high in calcium as grains or compost.

Yea, I've got a thousand of those. They cover everything but hands, feet, and front of face. Nice thick tyvek too.

Cake-a-day ritual for the habitual coffee drinkers, a never ending procession of invitros! Or if you work anywhere that serves a lot of coffee or know someone who does, big score of free food to mix half & half with verm & noc up. Just say it's "to keep ants away from your tomato garden"

I prefer baking in oven at 170F for a few hrs ...recently started using 1 gallon ziplocks which can be directly spawned and mixed with minimal contam risk....Just make sure you have accurate oven thermometer....Ziplocks could proly be used with Rodgers steaming cauldron tec too....

The yellow circles are what I eat - 10 mg each - one and you might feel funny (a little rubbery) but for any kind of a "fucked up" dose you'd have to eat three. Chances of feeling like Gumbi are high, chances of passing out within an hour are higher. I've been told to never ever ever drink on them. See info here http://cyclobenzaprine.drugs.com/

Those look cool to me except for maybe that little
greenish splotch in the first picture, but I can't
make it out; look pretty standard to me,
especially all that purple dust, I think the
splotch is just bluing. Are they as firm as they
look? Tear open a steam and check the center - if
it looks bluish-white then I'd say you have
winners, if it's black you have my condolences.

(Message edited by taoistshredder on January 26, 2005)

[pinwisher]

I put casting wet to field capacity in a tupperware bowl, crack the cover and nuke it for 5 minutes. I let it cool a bit, nuke again 5 minutes, and cover when it stops, I just let them cool overnight in the microwave.

I then take them and mix with pasturized straw, spawn, mix well, cover and away you go...

maybe 50/50 straw/castings. It's been a while, I'll have to doublecheck

(Message edited by pinwisher on January 26, 2005)

[petri]

Insight, a small mouth mason jar will fit your blender base. Add distilled water to the jar, put the blender base on it loosely, wrap in foil and pc/sterilize. This with the RR iodine tear technique, should give you great results.

[insight]

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

Insight, a small mouth mason jar will fit your blender base. Add distilled water to the jar, put the blender base on it loosely, wrap in foil and pc/sterilize.<!-/quote-!><hr size=0></blockquote>

This is the Tek I did before, however, part of the culture became contaminated as I wasnt able to sterilize the blender. Are you saying to take the culture, add to water, blend, the PC? Wont the excessive heat kill the culture?

[insight]

I read that wrong... put culture and water in jar, add base and sterilize (with blender base)on jar? How long and at what PSI? I dont want to melt the base

[bob]

Dont use a coffee filter use postal tyvek like the priority mail envelopse are made of

Thanks to you guys i just made 48 onzes of flour, i use to use whole rice because the rice fluor they sell here is white rice and the BRF i found in a health store wasn't flour it was like taking a whole long grains and cutting it in the middel with big chunks and by the way it will be my first time with pure "BRF". I think it will colonize faster because the nutrients are all over the vermiculite, because it is esayer to walk then jump!!!!!!!!!!!I Think!!!!!!!!!
Best wishes ............//////////////////////

[Lazlo]

I got some of those from Hawk when he first got those puppies and i'll agree, they are nice. I always fruit mine with a coir casing so it really gets overlaid bigtime. But they always notted up and when off. I just watered around the edge of the pan to keep it hydrated. Like you said, it will puddle up on the overlay and i think it does harm to any other pins trying to form. KSSS kick ass!!!

yeah mang, ksss is the jam, i did cakes with them, was crazy!

(Message edited by busst on January 26, 2005)

[Lazlo]

LOL! A brick! that's got me beat. Ghetto FABULOUS!!!

[insight]

I have a similar problem going on with some B+. All verm casing, mycilium running rapid on top...

[Lazlo]

You can add karo culture to the jar and then put the blender base on the jar loosely. Wrap the whole thing in foil and pc for 25 minutes @ 15psi for sterilization. Then put in gloove box and so on.

(Message edited by looper on January 26, 2005)

[Lazlo]

If you have to use filters, double them up. They will get plenty of gas exchange.

[Lazlo]

Since you guys are on the subject, how good do oyster shrooms taste? I might have had them and not even known it. I'm really curious about them, they are a beautiful mushroom and look very tasty. Could you give me an example of a dish they would be served with or a way to eat them. Are they a gourmet mushroom?

I normally don't work with tyvec. Has anyone noticed a difference in po tyvec and house wrap tyvec?

Rodger
One reason to have two layers of tyvec would be moisture wicking. If you have two layers with air space between the two layers its harder for the top layer to get wet.
The top layer would act as a contam barrier and the bottom layer would act as a water barrier.
Nothing going in and nothing coming out.

I remember a post at the Shroomery a while back. It was just a diagram but it showed three layers of tyvec on a liquid culture lid. The theory was that you could shake the hell out of it and the top layer would still not got wet.

Like I said, Ive never used tyvec so I don't know how it acts.
Does water just flow right through it?

[kevinfergo]

thx for the help

hhaaha ,....naw cut em all open no black in the middle they smell fine so if there not black on the inside TS there good to go, the only black i could find was on some of the caps i thought it was just spore prints,... spores dont rott on the shroom do they

[Lazlo]

I have a friend that has a South American print that I would like to get going in karo culture. My ? is, the print has been lying around for a while and i think it could have been handled incorrectly. Could i saaaaaay, put some spores in a solution of Betadine to help cleanse or something else. Then possibly transfer to the liquid cuture? Any help would be greatly appreciated!

The #65 "THE MUSHROOM CULTURE," The Journal Of Mushroom Cultivation (TMC)
January 2005 Issue is now up for free download. Just go to www.mushroomsfmrc.com and click "TMC Journals."
Below is a partial list of articles, some of which I hope you find informative.
Dr. Guzman gives a talk
Magic mushroom case judge tells prosecutor: "Chill Out"
Cordyceps capitata
Amanita Mycelium
Pleurotus ostreatus strains
"Where Have All the Frogs Gone?"
"Microbial Fermentation"
"Grain Contaminated With Aflatoxin Kills 50."
"Sequencing of the Genome of P. ramorum
"Immunoassays "
'Waviness'
"Naturally Occurring organohalogens"
"Glucatel"
"Mold Website"
"Sites About Mushrooms"
"Sudden Oak Death"
"TCA And Wine"
"The Fungus Discula destructiva"
"Carpet Monsters and Killer Spores"
"Extinction of Plants and Animals"
"Fungal Disease"
"The Artillery Fungus"
Will Mushrooms Replace Antibiotics In Animal Feed?
New Mushroom Growing Home
"Maitake Veggie Soup"
Functional properties of Grifola frondosa, Maitake
Japanese Researchers Use Mushrooms for Rubber
'Death Cap' Mushroom Leaves One Dead
Color Photograph Stropharia rugoso-annulata
Spore Print sample Clitocybe nuda
"Mushroom Terms" A FREE Download
Cultivation of Oyster Mushrooms
How Mushroom Spores Transport To New Areas On Earth
Thank you, now go and enjoy............slp/fmrc

There is a product FMRC makes available "MA". It is Gentimicin Sulfate in liquid form. Spores soaked in this for a day or two should be bacteria free. Go ahead and lay some out on agar. If contaminants show up, pour hot agar again over the project. The mycelium will bust out on top of the new layer, leaving the junk behind. Snag and transer the new growth to a clean dish. Rabbit might have some good ideas too, as he is becoming an expert on retrieving clean strains......grin.
This information applies to mushroom spores in general, and not any specific type. slp/fmrc

[Lazlo]

Thanks alot man. So if i get some contams on agar simply pour another, hot layer of agar right on top? The heat won't damage the mycellium?

[llama]

Thanks Stephen!
By the way, oyster mycelia from the print you sent are running fast on my agar. Thanks again for that too.

[llama]

Anyone know the ratio of fruiting to non-fruiting strains for oysters?

That's a good little mushroom grasshopper...slp/fmrc

No, the mycelium will be fine. Other crap might die though, but that's a good thing....slp/fmrc

[nomoreusmc]

I don't know If I can qualify those results rodger, you can grow shrooms an anything!!! Just kidding!!

would it be possible to go ahead and noc the karo with the dirty spores, and then clean up the culture later with some H202 in the syringe?
The only reason I ask is cuz I think I read something like that in the archives once upon a time...

[sparky]

not exactly, if I may...

Put the water in a small mouth jar and screw the blender base on it. Cover the blender base with foil and sterilize it in the PC.

When it cools, <then> put in the sample of the stem and blend it into a solution, dont PC the culture......

}}

i would never have thought that a blender could be cooked in the pc - i guess you've done it before, right? of course, you would have to pc a lid also, and you can only do one jar at a time that way. sounds fool proof though. I wonder how well the glass shards work.

Does this straw pasteuization technique really work?
...the one where you soak it for a day or so in lime water, then drain, all at room temps. anyone have exp. with it?

[1dumbmyco]

Great idea there Rodger and thanks for a quote from the epitome of music from Eric Burden. Damn I still love that song. Once moved I will beg some coffee grounds from my neighbor and see what I can do with this innovation.

Could a % of grains be substituted for the lack of calcium if needed?

[sparky]

ya dont put the blender picher in the PC if thats what ya mean.

I just do one jar of water in with the jars of grain for a cloning session, it works great.

theres a huge suicide rate amoung teens getting addicted in Canada, but i think its because the doctors were dumb fucks giving them like unlimited quantity for a broken wrist,

i know Taoist you were sayin that t3s are what you get for wisdom teeth, but i know around here you can tell your doctor you want somthing stronger ,.. and theyll give you the Oxicocets or oxicontin no questions asked..

hey TheFungiOne whats the scoop on ativan i got a bunch of them ,.. wwill they give any buzz

[shobimono]

Besides being a substitute for flour, I think the more important result is that this may be a boon for people who do not have access to a pressure cooker.

Rodger stated he only boiled the jars, no pc, no fractional nonsense, just boiling. I would guess this works because the coffee ground/verm is less dense than BRF (flour+water="glue") is. The center of a coffee ground/verm cake probably gets hotter more quickly than a BRF cake would.

I think this would also mean that this probably wouldn't work with larger jars unless you pc'ed them. Also if you added more things like grains, or any flour, you would probably have to pc it.

[shobimono]

Stephen where does the FMRC make the MA available? I couldn't find it on the website.

[Lazlo]

It doesn't make any difference at all wether you pc karo culture or just plain water in the jar. If you plan on storring the blended mushroom solution you need nutrients, Karo or any other choice. If you aren't going to store it then simply use H2O alone.

[Lazlo]

Thanks very much Stephen, much appreciative!!!

(Message edited by looper on January 27, 2005)

<blockquote><hr size=0><!-quote-!><font size=1>quote:</font>

would it be possible to go ahead and noc the karo with the dirty spores, and then clean up the culture later with some H202 in the syringe?<!-/quote-!><hr size=0></blockquote>
You need agar for that work.

I can vouch for stephen's hot agar tek. It really does work, and works very well. The hot agar completely envelops the healthy mycelium and the contaminants. The mushroom mycelium crawls out from under the agar, leaving the contaminants behind.

[1dumbmyco]

They taste great alone or as an addition to other dishes. Not quite a morel, but very delicious.