culturing yeast

Hippie3 · 09-14-09, 00:45

say i drop the $$$ for some yeast that can tolerate 19-20% ethanol content,
can it be grown out to use later or do i gotta buy each time ?
if it can be grown-
how ?

Phx · 09-14-09, 00:55

Thats a great question Hip. I usually just trash my left over yeast. I just bought some chanpagne yeast which the local brew shop said it would reach 18-20. I don't know what the differance is between my yeast and the distillers yeast though.

kcmoxtractor · 09-14-09, 01:06

http://en.wikipedia.org/wiki/Yeast#Growth_and_nutrition

Apparently on PDA and in a "liquid broth" which I'm pretty sure would be a LC.

kattertjie · 09-14-09, 04:22

From kcmoxtractor's yeast link:
In industrial brewing, to ensure purity of strain, a 'clean' sample of the yeast is stored refrigerated in a laboratory. After a certain number of fermentation cycles, a full scale propagation is produced from this laboratory sample. Typically, it is grown up in about three or four stages using sterile brewing wort and oxygen.

Also check out: http://en.wikipedia.org/wiki/Pasteur_effect

I never even thought of propagating from one batch of yeast. Then again, I have never bought a super special expensive yeast. Sounds like you can make a LC using malt extract... only need to keep it oxygenated so it doesn't ferment (at least during the growing stages). Pretty cool idea hip!

Hippie3 · 09-14-09, 08:53

lc it is then,
thx

essentrik1 · 09-15-09, 20:36

http://www.thebrewingnetwork.com has some great resources on yeast use

The Sunday Session in chronological order:
Yeast w/ Chris White of White Labs
Yeast with Dave Logsdon
Yeast w/ the White's #2
Yeast with White Labs Yeast Co.
Non-Traditional Yeast

Those are the first couple and the ones I remember as being good.
http://thebrewingnetwork.com/shows/The-Sunday-Session/search/yeast
for more

abaca · 09-17-09, 10:44

There is a good book on the subject call 'Yeast Culture' I believe. It will tell you everything you need to know, pictures and step by step procedure; a great reference to have. IMHO if you can clone a mushroom you can culture yeast, much of the same procedure. This is for long term storage, if you plan on using the yeast within 2 weeks just save the yeast from the bottom of the fermenter. Steralize a mason jar & any other equipment that will touch the yeast, transfer yeast into the jar, seal it loosley and keep it in the fridge.

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09-14-09, 00:45	#1 (permalink)
Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 43,266	culturing yeast say i drop the $$$ for some yeast that can tolerate 19-20% ethanol content, can it be grown out to use later or do i gotta buy each time ? if it can be grown- how ? __________________ visit dial-a-hippie .

09-14-09, 00:55	#2 (permalink)
Phx Mycotopiate Join Date: Jul 2008 Posts: 405	Thats a great question Hip. I usually just trash my left over yeast. I just bought some chanpagne yeast which the local brew shop said it would reach 18-20. I don't know what the differance is between my yeast and the distillers yeast though. __________________ Phx

09-14-09, 01:06	#3 (permalink)
kcmoxtractor Satan's Helper Join Date: Jun 2008 Posts: 1,349	http://en.wikipedia.org/wiki/Yeast#Growth_and_nutrition Apparently on PDA and in a "liquid broth" which I'm pretty sure would be a LC. __________________ "It was the straying that found the path direct" - Austin Osman Spare

09-14-09, 08:53	#5 (permalink)
Hippie3 DUNG DEALER Join Date: Feb 2001 Posts: 43,266	lc it is then, thx __________________ visit dial-a-hippie .

09-14-09, 04:22	#4 (permalink)
kattertjie Mycophiliac Join Date: Jun 2009 Posts: 39	From kcmoxtractor's yeast link: In industrial brewing, to ensure purity of strain, a 'clean' sample of the yeast is stored refrigerated in a laboratory. After a certain number of fermentation cycles, a full scale propagation is produced from this laboratory sample. Typically, it is grown up in about three or four stages using sterile brewing wort and oxygen. Also check out: http://en.wikipedia.org/wiki/Pasteur_effect I never even thought of propagating from one batch of yeast. Then again, I have never bought a super special expensive yeast. Sounds like you can make a LC using malt extract... only need to keep it oxygenated so it doesn't ferment (at least during the growing stages). Pretty cool idea hip!

09-15-09, 20:36	#6 (permalink)
essentrik1 Mycophiliac Join Date: Jan 2009 Posts: 54	http://www.thebrewingnetwork.com has some great resources on yeast use The Sunday Session in chronological order: Yeast w/ Chris White of White Labs Yeast with Dave Logsdon Yeast w/ the White's #2 Yeast with White Labs Yeast Co. Non-Traditional Yeast Those are the first couple and the ones I remember as being good. http://thebrewingnetwork.com/shows/The-Sunday-Session/search/yeast for more

09-17-09, 10:44	#7 (permalink)
abaca jerk of all trades Join Date: Nov 2005 Posts: 335	There is a good book on the subject call 'Yeast Culture' I believe. It will tell you everything you need to know, pictures and step by step procedure; a great reference to have. IMHO if you can clone a mushroom you can culture yeast, much of the same procedure. This is for long term storage, if you plan on using the yeast within 2 weeks just save the yeast from the bottom of the fermenter. Steralize a mason jar & any other equipment that will touch the yeast, transfer yeast into the jar, seal it loosley and keep it in the fridge. __________________

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